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Journal of Integrative Agriculture  2026, Vol. 25 Issue (9): 3725-3735    DOI: 10.1016/j.jia.2025.11.013
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The Clygp (yellow-green plant) encodes a signal recognition particle 54 kDa protein modulating chloroplast development and photosynthesis in watermelon

Shixiang Duan1, 2*, Yaomiao Guo1, 2*, Lin Deng1, 2, Qishuai Kang1, 2, Changbao Shen1, 2, Xiaohang Xue1, 2, Junling Dou1, 2, Dongming Liu1, 2, Sen Yang1, 2, Xingping Zhang4, Yun Deng4, Huayu Zhu1, 2#, Yongdong Sun3#, Luming Yang1, 2#

1 College of Horticulture, Henan Agricultural University, Zhengzhou 450046, China

2 Research Center of Cucurbit Germplasm Enhancement and Utilization of Henan Province, Zhengzhou 450046, China

3 School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China

4 Peking University Institute of Advanced Agricultural Sciences, Weifang 261325, China

 Highlights 
A novel chlorophyll-deficient yellow-green mutant PKH352 was identified from an EMS-induced watermelon mutant library.
Mutation in ClG42_04g0106300 gene, encoding a signal recognition particle 54 kDa protein, leads to the yellow-green phenotype and reduced photosynthetic efficiency in watermelon.
The ClG42_04g0106300 gene plays an essential regulatory role in chloroplast biogenesis and chlorophyll metabolism in watermelon.
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摘要  

光合作用作为高等植物营养物质合成的主要来源,提高光合效率有助于显著提高作物的产量和果实品质。叶色突变体作为研究高等植物叶绿体发育和光合作用机制的理想材料,在大田作物中被广泛研究,但其在西瓜中的研究与应用十分有限。本研究从西瓜EMS诱变突变体库中筛选获得一个黄绿表型突变体PKH352,其叶绿素含量和最大光化学效率显著降低。遗传分析表明该突变体的黄绿性状受隐性单基因调控,命名为ClygpCitrullus lanatus yellow-green plant)。通过在440株的F2群体中进行MutMap和连锁分析,我们鉴定出ClG42_04g0106300基因上的单核苷酸多态性(SNP)突变为PKH352黄绿表型变异的原因,该基因编码一个叶绿体信号识别颗粒54kD亚基蛋白。利用CRISPR/Cas9基因编辑体系验证,敲除ClG42_04g0106300基因导致西瓜出现黄绿表型,进一步验证了该基因的功能。此外,转录组分析显示,ClG42_04g0106300基因突变显著影响叶绿体发育和光合作用中相关基因的表达,充分表明其在这些生物学通路中起重要作用。综上所述,本研究为解析西瓜叶绿体发育和光合效率的分子机制提供了新的基因资源,为培育高光合效率西瓜品种奠定了理论基础。



Abstract  

Photosynthesis serves as the primary source of nutrients synthesized in higher plants, and improving photosynthetic efficiency can significantly increase crop yield and fruit quality.  Leaf color mutants represent ideal materials for studying chloroplast development and photosynthesis mechanisms and have been widely characterized in field crops.  However, relevant research on watermelon leaf color mutants remains scarce.  In this study, we isolated a yellow-green phenotype mutant, PKH352, from an EMS-mutagenized watermelon mutant library.  The chlorophyll content and maximal photochemical efficiency in PKH352 were significantly decreased.  Genetic analysis showed that the mutated trait was controlled by a single nuclear gene, which was named Clygp (Citrullus lanatus yellow-green plant).  Through MutMap and linkage analysis in an F2 population of 440 plants, we identified a single nucleotide polymorphism (SNP) mutation within ClG42_04g0106300, which encoded a signal recognition particle 54 kDa protein, as the causal variant for the yellow-green phenotype.  Further validation using a CRISPR/Cas9-mediated system confirmed that knockout of ClG42_04g0106300 results in the yellow-green phenotype in watermelon.  In addition, comparative transcriptomic analysis revealed that mutations in ClG42_04g0106300 greatly affected the expression of key genes associated with chloroplast development and photosynthesis, providing strong evidence that this gene plays a critical role in these biological pathways.  Taken together, these findings provide insights into the molecular mechanisms underlying chloroplast development and photosynthetic efficiency, offering a theoretical basis for breeding watermelon varieties with high photosynthetic efficiency.

Keywords:  watermelon       yellow-green phenotype        photosynthesis        fine-mapping        gene editing  
Received: 19 April 2025   Accepted: 29 August 2025 Online: 13 November 2025  
Fund: 

This research was supported by the National Natural Science Foundation of China (32172602, 32472739), the Excellent Youth Foundation of Henan Scientific Committee, China (242300421030), and the Science and Technology Project of the Tibetan Plateau Seed Breeding Technology Innovation Center, China (LSQSCNYQ2025006). 


About author:  Shixiang Duan, E-mail: sxiangduan@163.com; Yaomiao Guo, E-mail: yaomiaoguo@163.com; #Correspondence Huayu Zhu, E-mail: hyzhu@henau.edu.cn; Yongdong Sun, E-mail: sunyd2001@163.com; Luming Yang, E-mail: lumingyang@henau.edu.cn * These authors contributed equally to this study.

Cite this article: 

Shixiang Duan, Yaomiao Guo, Lin Deng, Qishuai Kang, Changbao Shen, Xiaohang Xue, Junling Dou, Dongming Liu, Sen Yang, Xingping Zhang, Yun Deng, Huayu Zhu, Yongdong Sun, Luming Yang. 2026. The Clygp (yellow-green plant) encodes a signal recognition particle 54 kDa protein modulating chloroplast development and photosynthesis in watermelon. Journal of Integrative Agriculture, 25(9): 3725-3735.

Bischoff A, Ortelt J, Dünschede B, Zegarra V, Bedrunka P, Bange G, Schünemann D. 2024. The role of chloroplast SRP54 domains and its C-terminal tail region in post-and cotranslational protein transport in vivoJournal of Experimental Botany75, 5734–5749.

Cackett L, Luginbuehl L H, Schreier T B, LopezJuez E, Hibberd J M. 2021. Chloroplast development in green plant tissues: The interplay between light, hormone, and transcriptional regulation. New Phytologist233, 2000–2016.

Cardona T, Shao S, Nixon P. 2018. Enhancing photosynthesis in plants: The light reactions. Essays in Biochemistry62, 85–94.

Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y H, Xia R. 2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant13, 1194–1202.

Chen F, Dong G J, Wu L M, Wang F, Yang X Z, Ma X H, Wang H L, Wu J H, Zhang Y L, Wang H Z, Qian Q, Yu Y C. 2016. A nucleus-encoded chloroplast protein YL1 is involved in chloroplast development and efficient biogenesis of chloroplast ATP synthase in rice. Scientific Reports6, 32295.

Croce R, Carmo-Silva E, Cho Y B, Ermakova M, Harbinson J, Lawson T, McCormick A J, Niyogi K K, Ort D R, Patel-Tupper D, Pesaresi P, Raines C, Weber A P M, Zhu X. 2024. Perspectives on improving photosynthesis to increase crop yield. Plant Cell36, 3944–3973.

Cutolo E A, Guardini Z, Dall’Osto L, Bassi R. 2023. A paler shade of green: Engineering cellular chlorophyll content to enhance photosynthesis in crowded environments. New Phytologist239, 1567–1583.

Deng Y, Liu S C, Zhang Y L, Tan J S, Li X P, Chu X, Xu B H, Tian Y, Sun Y D, Li B S, Xu Y B, Deng X W, He H, Zhang X. 2022. A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding. Molecular Plant15, 1268–1284.

Dou J L, Yang H H, Sun D L, Yang S, Sun S R, Zhao S J, Lu X Q, Zhu H Y, Liu D M, Ma C S, Liu W G, Yang L M. 2022. The branchless gene Clbl in watermelon encoding a TERMINAL FLOWER 1 protein regulates the number of lateral branches. Theoretical and Applied Genetics135, 65–79.

Duan S X, Guo Y M, Wang Y P, Umer M J, Liu D, Yang S, Niu H H, Sun S R, Yang L M, Dou J L, Zhu H Y. 2023. HD-Zip transcription factor is responsible for no-lobed leaf in watermelon (Citrullus lanatus L.). Phyton-International Journal of Experimental Botany18, 1311–1328.

Fekih R, Takagi H, Tamiru M, Abe A, Natsume S, Yaegashi H, Sharma S, Sharma S, Kanzaki H, Matsumura H, Saitoh H, Mitsuoka C, Utsushi H, Uemura A, Kanzaki E, Kosugi S, Yoshida K, Cano L, Kamoun S, Terauchi R. 2013. MutMap+: Genetic mapping and mutant identification without crossing in rice. PLoS ONE8, e68529.

Gebremeskel H, Umer M J, Zhu H J, Li B B, Zhao S G, Yuan P L, Lu X Q, Nan H, Liu W G. 2023. Genetic mapping and molecular characterization of the delayed green gene dg in watermelon (Citrullus lanatus). Frontiers in Plant Science14, 1152644.

He G, Zhang R, Jiang S H, Wang H H, Ming F. 2023. The MYB transcription factor RcMYB1 plays a central role in rose anthocyanin biosynthesis. Horticulture Research10, uhad080.

Hristou A, Gerlach I, Stolle D S, Neumann J, Bischoff A, Dünschede B, Nowaczyk M M, Zoschke R, Schünemann D. 2019. Ribosome-associated chloroplast SRP54 enables efficient cotranslational membrane insertion of key photosynthetic proteins. Plant Cell31, 2734–2750.

Hu L L, Zhang H Q, Xie C, Wang J, Zhang J Y, Wang H, Weng Y Q, Chen P, Li Y H. 2020. A mutation in CsHD encoding a histidine and aspartic acid domain-containing protein leads to yellow young leaf-1 (yyl-1) in cucumber (Cucumis sativus L.). Plant Science293, 110407.

Jeong J, Baek K, Kirst H, Melis A, Jin E. 2017. Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtiiBiochimica et Biophysica Acta-Bioenergetics1858, 45–55.

Ji S L, Grimm B, Wang P. 2023. Chloroplast SRP43 and SRP54 independently promote thermostability and membrane binding of light-dependent protochlorophyllide oxidoreductases. Plant Journal115, 1583–1598.

Ji S L, Siegel A, Shan S O, Grimm B, Wang P. 2021. Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock. Nature Plants7, 1420–1432.

Kirst H, Melis A. 2014. The chloroplast signal recognition particle (CpSRP) pathway as a tool to minimize chlorophyll antenna size and maximize photosynthetic productivity. Biotechnology Advances32, 66–72.

Lan Y H, Song Y, Zhao F, Cao Y, Luo D L, Qiao D R, Cao Y, Xu H. 2022. Phylogenetic, structural and functional evolution of the LHC gene family in plant species. International Journal of Molecular Sciences24, 488.

Lei Y, Li B L, Wang X M, Wei J Y, Wang P Y, Zhao J, Yu F F, Qi Y F. 2023. Chloroplast SRP54 and FtsH protease coordinate thylakoid membrane-associated proteostasis in Arabidopsis. Plant Physiology192, 2318–2335.

Levin G, Schuster G. 2023. LHC-like proteins: The guardians of photosynthesis. International Journal of Molecular Sciences24, 2503.

Li C R, Du X J, Liu C M. 2025. Enhancing crop yields to ensure food security by optimizing photosynthesis. Journal of Genetics and Genomics52, 1082–1095

Li Q L, Wang X M, Lei Y, Wang Y L, Li B L, Liu X Y, An L J, Yu F, Qi Y F. 2022. Chloroplast envelope ATPase PGA1/AtFtsH12 is required for chloroplast protein accumulation and cytosol-chloroplast protein homeostasis in Arabidopsis. Journal of Biological Chemistry298, 102489.

Li W R, Zhang Y C, Mazumder M A R, Pan R H, Akhter D. 2022. Research progresses on rice leaf color mutants. Crop Design1, 100015.

Lichtenthaler H K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. In: Methods in Enzymology. Academic Press, USA. pp. 350–382.

Liu D M, Liang J F, Liu Q Q, Chen Y X, Duan S X, Sun D L, Zhu H Y, Dou J L, Niu H H, Yang S, Sun S R, Hu J B, Yang L L. 2025. The pseudo-type response regulator gene Clsc regulates rind stripe coloration in watermelon. Journal of Integrative Agriculture24, 147–160.

Liu D M, Yang H H, Yuan Y X., Zhu H Y, Zhang M J, Wei X C, Sun D L, Wang X J, Yang S, Yang L M. 2020. Comparative transcriptome analysis provides insights into yellow rind formation and preliminary mapping of the Clyr (yellow rind) gene in watermelon. Frontiers in Plant Science11, 192.

Lu H J, Xiao Y Y, Liu Y X, Zhang J C, Zhao Y Y. 2024. Integrative transcriptomics and proteomics analysis of a cotton mutant yl1 with a chlorophyll-reduced leaf. Plants-Basel13, 1789.

Ma Y Y, Shi J C, Wang D J, Liang X, Wei F, Gong C M, Qiu L J, Zhou H C, Folta K M, Wen Y Q, Feng J Y. 2023. A point mutation in the gene encoding magnesium chelatase I subunit influences strawberry leaf color and metabolism. Plant Physiology192, 2737–2755.

Naito Y, Hino K, Bono H, Ui-Tei K. 2015. CRISPR direct: Software for designing CRISPR/Cas guide RNA with reduced off-target sites. Bioinformatics31, 1120–1123.

Neff M M, Turk E, Kalishman M. 2002. Web-based primer design for single nucleotide polymorphism analysis. Trends in Genetics18, 613–615.

Niu H H, Li P F, Zhang M J, Meng H, Wang H, Yan W K, Liu D M, Dou J L, Yang S, Zhu H Y, Yang L M, He S. 2024. Genome-wide identification of ClSPL gene family and functional characterization of ClSPL9 in watermelon. Scientia Horticulturae337, 113539.

Pan J W, Song J, Sharif R, Xu X W, Li S T, Chen X H. 2024. A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype. Journal of Integrative Agriculture23, 849–862.

Rocha J J, Jayaram S A, Stevens T J, Muschalik N, Shah R D, Emran S, Robles C, Freeman M, Munro S. 2023. Functional unknomics: Systematic screening of conserved genes of unknown function. Plos Biology21, e3002222.

Schünemann D. 2004. Structure and function of the chloroplast signal recognition particle. Current Genetics44, 295–304.

Tang D D, Chen M J, Huang X H, Zhang G C, Zeng L, Zhang G S, Wu S J, Wang Y W. 2023. SRplot: A free online platform for data visualization and graphing. PLoS ONE18, e0294236.

Walter B, Pieta T, Schünemann D. 2015. Arabidopsis thaliana mutants lacking cpFtsY or cpSRP54 exhibit different defects in photosystem II repair. Frontiers in Plant Science6, 250.

Wang F, Duan S M, Tong L, Wang R N, Tao Y S. 2018. Fine mapping and candidate gene analysis of leaf color mutant in maize. Journal of Plant Genetic Resources6, 1205–1209.

Wang L F, Sun J T, Wang C Y, Shangguan Z P. 2018. Leaf photosynthetic function duration during yield formation of large-spike wheat in rainfed cropping systems. PeerJ6, e5532.

Wang Y, An Z, Li R, Yang X, Huang Y, Shao R, Ye Y. 2019. The nutritional status and fluorescence characteristics of maize cultivars with different chlorophyll content and yields. Photosynthetica57, 295–302.

Yang J Y, Chen Y T, Sun X W, Zhang X Y, Wang S Y, Lyu Y P, Hu Y J, Wang X X. 2025. Curly leaf 1, a CHD domain-containing protein, regulates leaf development by H3K27me3 modification in rice. The Crop Journal2, 360–371.

Yang S, Wang X J, Yan W K, Zhang Y, Song P Y, Guo Y M, Xie K X, Hu J B, Hou J, Wu Y F, Zhu H Y, Sun S R, Yang L M. 2023. Melon yellow-green plant (Cmygp) encodes a Golden2-like transcription factor regulating chlorophyll synthesis and chloroplast development. Theoretical and Applied Genetics136, 66.

Yang S, Wang Y L, Zhu H Y, Zhang M J, Wang D K, Xie K X, Fan P F, Dou J L, Liu D M, Liu B, Chen C H, Yan Y, Zhao L J, Yang L M. 2022. A novel HD-Zip I/C2H2-ZFP/WD-repeat complex regulates the size of spine base in cucumber. The New Phytologist233, 2643–2658.

Yang W Z, Yuan Y H, Yang P J, Li S Z, Ma S, Liu X Q, Zhou X J, Chen R M. 2023. ZmGluTR1 is involved in chlorophyll biosynthesis and is essential for maize development. Journal of Plant Physiology290, 154115.

Yue L Q, Zhong M, Kang D J, Qin H Y, Li H C, Chai X, Kang Y Y, Yang X. 2024. Genome-wide identification and characterization of flavonol synthase (FLS) gene family in Brassica vegetables and their roles in response to biotic and abiotic stresses. Scientia Horticulturae, 331, 113168.

Zhang C, Zhang J X, Tang Y J, Liu K W, Liu Y, Tang J Q, Zhang T, Yu H X. 2021. DEEP GREEN PANICLE1 suppresses GOLDEN2-LIKE activity to reduce chlorophyll synthesis in rice glumes. Plant Physiology185, 469–477.

Zhang R X, Zhang N N, Wang Y X, Abid K, Shuai M A, Bai X, Zeng Q, Pan Q M, BaoHua L, Zhang L G. 2023. Blue light induces leaf color change by modulating carotenoid metabolites in orange-head chinese cabbage (Brassica rapa L. ssp. pekinensis). Journal of Integrative Agriculture22, 3296–3311.

Zhang T Q, Xiao W W, Wang Z W, Zhang J C, Shen W Q, Tu R R, Wu R H, Zhou K, Sang X C, Ling Y H, He G H, Zhang T. 2025. YGL9 mediates LHC assembly by regulating LHCPs transport and chlorophyll synthesis in rice. Plant Journal121, e17256.

Zhang T T, Dong X Y, Yuan X, Hong Y Y, Zhang L L, Zhang X, Chen S X. 2022. Identification and characterization of CsSRP43, a major gene controlling leaf yellowing in cucumber. Horticulture Research9, uhac212.

Zhao M H, Li X, Zhang X X, Zhang H, Zhao X Y. 2020. Mutation mechanism of leaf color in plants: A review. Forests11, 851.

Zhu H Y, Zhang M J, Sun S R, Yang S, Li J X, Li H, Yang H H, Zhang K G, Hu J B, Liu D M, Yang L M. 2019. A single nucleotide deletion in an ABC transporter gene leads to a dwarf phenotype in watermelon. Frontiers in Plant Science10, 1399.

Zhu Y C, Yuan G P, Wang Y F, An G L, Li W H, Liu J P, Sun D X. 2022. Mapping and functional verification of leaf yellowing genes in watermelon during whole growth period. Frontiers in Plant Science13, 1049114.

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Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon (Citrullus lanatus)
[J]. >Journal of Integrative Agriculture, 2020, 19(2): 528-540.
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