Transcriptomic and metabolomic analysis of changes in grain weight potential induced by water stress in wheat

doi:10.1016/j.jia.2023.12.015

Journal of Integrative Agriculture

2024, Vol. 23

Issue (11): 3706-3722 DOI: 10.1016/j.jia.2023.12.015

Special Issue: 小麦耕作栽培Wheat Physiology · Biochemistry · Cultivation · Tillage

Crop Science

Advanced Online Publication | Current Issue | Archive | Adv Search

Transcriptomic and metabolomic analysis of changes in grain weight potential induced by water stress in wheat

Yanmei Gao^{1, 3}, Maoya Jing¹, Meng Zhang¹, Zhen Zhang², Yuqing Liu², Zhimin Wang^2#, Yinghua Zhang^2#

¹School of Life Science, Shanxi Normal University, Taiyuan 030031, China

²College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China

³Ministerial and Provincial Co-Innovation Centre for Endemic Crops Production with High-quality and Efficiency in Loess Plateau, Taigu 030801, China

Abstract
References

Download: PDF in ScienceDirect
Export: BibTeX | EndNote (RIS)

摘要

小麦子房发育的库强决定了粒重潜力。从孕穗到籽粒建成期是子房生长发育和潜在库强决定的关键时期。然而，水分胁迫下，小麦植株在这一时期如何平衡和协调小花数和子房/籽粒重的潜在调控机制尚不清楚。因此，我们设计了W0（生长季不灌溉）和W1（拔节期灌溉75 mm）两个灌溉处理，从表型、代谢和转录组水平分析了小麦子房/籽粒重对水分胁迫的响应。结果表明，W0处理降低了土壤含水量、株高和旗叶叶面积，从而减少了粒数，尤其是弱势粒的粒数，但是提高了强势粒和弱势粒的粒重以及成熟期籽粒的平均粒重，与此同时，花前3天到花后10天子房/籽粒的重量和体积也都增加了。转录组分析表明，W0处理显著促进了蔗糖代谢和植物激素信号转导相关基因的表达，并导致可溶性酸转化酶(SAI)和蔗糖合酶(Sus)活性以及脱落酸(ABA)和吲哚-3-乙酸(IAA)水平升高，因此蔗糖含量降低，葡萄糖和果糖含量增加。此外，在W0处理下，花前几个TaTPP基因（尤其是TaTPP-6）下调，IAA生物合成基因TaTAR1和TaTAR2上调，这进一步提高了IAA水平。总的来说，水分胁迫减少了营养器官的生长，导致大部分弱势粒退化，但是提高了存活的子房/籽粒的ABA和IAA水平，提升了Sus酶的活性，将蔗糖降解为葡萄糖和果糖。由此可见，在水分胁迫下，较强的蔗糖利用能力，增强的SAI酶活性以及ABA和IAA介导的信号转导共同提高了小麦存活子房/籽粒的重量和体积，最终实现了子房/籽粒重和粒数之间的平衡。

Abstract

The sink strength of developing ovaries in wheat determines the grain weight potential. The period from booting to the grain setting stage is critical for ovary growth and development and potential sink capacity determination. However, the underlying regulatory mechanism during this period by which the wheat plant balances and coordinates the floret number and ovary/grain weight under water stress has not been clarified. Therefore, we designed two irrigation treatments of W0 (no seasonal irrigation) and W1 (additional 75 mm of irrigation at the jointing stage) and analyzed the responses of the ovary/grain weight to water stress at the phenotypic, metabolomic, and transcriptomic levels. The results showed that the W0 irrigation treatment reduced the soil water content, plant height, and green area of the flag leaf, thus reducing grain number, especially for the inferior grains. However, it improved the grain weight of the superior and inferior grains as well as average grain weight at maturity, while the average ovary/grain weight and volume during –3 to 10 days after anthesis (DAA) also increased. Transcriptomic analysis indicated that the genes involved in both sucrose metabolism and phytohormone signal transduction were prominently accelerated by the W0 treatment, accompanied by greater enzymatic activities of soluble acid invertase (SAI) and sucrose synthase (Sus) and elevated abscisic acid (ABA) and indole-3-acetic acid (IAA) levels. Thus, the sucrose content decreased, while the glucose and fructose contents increased. In addition, several TaTPP genes (especially TaTPP-6) were down-regulated and the IAA biosynthesis genes TaTAR1 and TaTAR2 were up-regulated under the W0 treatment before anthesis, which further increased the IAA level. Collectively, water stress reduced the growth of vegetative organs and eliminated most of the inferior grains, but increased the ABA and IAA levels of the surviving ovaries/grains, promoting the enzymatic activity of Sus and degrading sucrose into glucose and fructose. As a result, the strong sucrose utilization ability, the enhanced enzymatic activity of SAI and the ABA- and IAA-mediated signaling jointly increased the weight and volume of the surviving ovaries/grains, and ultimately achieved the trade-off between ovary/grain weight and number in wheat under water stress.

Keywords: grain weight sucrose metabolism endogenous hormones water stress transcriptome

Received: 01 August 2023 Accepted: 13 November 2023

Fund:

This research program was sponsored by the Ministerial and Provincial Co-Innovation Centre for Endemic Crops Production with High-quality and Efficiency in Loess Plateau, China (SBGJXTZX-44), the Fundamental Research Program of Shanxi Province, China (20210302124237), the National Key Research and Development Program of China (2022YFD2300802), and the China Agriculture Research System (CARS-3). Financial support from the above sources is gratefully acknowledged.

About author: Yanmei Gao, Tel: +86-351-2051196, E-mail: gaoyanmei0225@ 163.com; #Correspondence Zhimin Wang, Tel: +86-10-62733399, E-mail: wangzm@cau.edu.cn; Yinghua Zhang, Tel: +86-10-62732431, E-mail: zhangyh1216@126.com

	Service
	E-mail this article
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Yanmei Gao
	Maoya Jing
	Meng Zhang
	Zhen Zhang
	Yuqing Liu
	Zhimin Wang
	Yinghua Zhang

Cite this article:

Yanmei Gao, Maoya Jing, Meng Zhang, Zhen Zhang, Yuqing Liu, Zhimin Wang, Yinghua Zhang. 2024. Transcriptomic and metabolomic analysis of changes in grain weight potential induced by water stress in wheat. Journal of Integrative Agriculture, 23(11): 3706-3722.

Acreche M M, Slafer G A. 2006. Grain weight response to increases in number of grains in wheat in a Mediterranean area. Field Crops Research, 98, 52–59.

Ahmadi A, Baker D A. 1999. Effects of abscisic acid (ABA) on grain filling processes in wheat. Plant Growth Regulation, 28, 187–197.

Arathi H S. 2011. Selective embryo abortion in a perennial tree-legume: A case for maternal advantage of reduced seed number per fruit. Journal of Plant Research, 124, 675–681.

Basunia M A, Nonhebel H M. 2019. Hormonal regulation of cereal endosperm development with a focus on rice (Oryza sativa). Functional Plant Biology, 46, 493–506.

Bernardi J, Battaglia R, Bagnaresi P, Lucini L, Marocco A. 2019. Transcriptomic and metabolomic analysis of ZmYUC1 mutant reveals the role of auxin during early endosperm formation in maize. Plant Science, 281, 133–145.

Borrás L, Slafer G A, Otegui M E. 2004. Seed dry weight response to source–sink manipulations in wheat, maize and soybean: A quantitative reappraisal. Field Crops Research, 86, 131–146.

Boussora F, Allam M, Guasmi F, Ferchichi A, Rutten T, Hansson M, Youssef H M, Börner A. 2019. Spike developmental stages and ABA role in spikelet primordia abortion contribute to the final yield in barley (Hordeum vulgare L.). Botanical Studies, 60, 13–24.

Brinton J, Uauy C. 2019. A reductionist approach to dissecting grain weight and yield in wheat. Journal of Integrative Plant Biology, 61, 337–358.

Calderini D F, Abeledo L G, Savin R, Slafer G A. 1999. Effect of temperature and carpel size during pre-anthesis on potential grain weight in wheat. Journal of Agricultural Science, 132, 453–459.

Calderini D F, Castillo F M, Arenas M A, Molero G, Reynolds M P, Craze M, Bowden S, Milner M J, Wallington E J, Dowle P, Gomez L D, McQueen-Mason S J. 2020. Overcoming the trade-off between grain weight and number in wheat by the ectopic expression of expansion in developing seeds leads to increased yield potential. New Phytologist, 230, 629–640.

Calderini D F, Savin R, Abeledo L G, Reynolds M P, Slafer G A. 2001. The importance of the period immediately preceding anthesis for grain weight determination in wheat. Euphytica, 119, 199–204.

Carmo-Silva E, Andralojc P J, Scales J C, Driever S M, Mead A, Lawson T, Raines C A, Parry M A J. 2017. Phenotyping of field-grown wheat in the UK highlights contribution of light response of photosynthesis and flag leaf longevity to grain yield. Journal of Experimental Botany, 68, 3473–3486.

Du H, Wu N, Chang Y, Li X H, Xiao J H, Xiong L Z. 2013. Carotenoid deficiency impairs ABA and IAA biosynthesis and differentially affects drought and cold tolerance in rice. Plant Molecular Biology, 83, 475–488.

Ehdaie B, Alloush G A, Waines J G. 2008. Genotypic variation in linear rate of grain growth and contribution of stem reserves to grain yield in wheat. Field Crops Research, 106, 34–43.

Fichtner F, Lunn J E. 2021. The role of trehalose 6-phosphate (Tre6P) in plant metabolism and development. Annual Review of Plant Biology, 72, 737–760.

Gao Y M, Zhang M, Yao C S, Liu Y Q, Wang Z M, Zhang Y H. 2021. Increasing seeding density under limited irrigation improves crop yield and water productivity of winter wheat by constructing a reasonable population architecture. Agricultural Water Management, 253, 106951.

Garcia P M, Asega A F, Silva E A, Carvalho M A M. 2011. Effect of drought and re-watering on fructan metabolism in Vernonia herbacea (Vell.) Rusby. Plant Physiology and Biochemistry, 49, 664–670.

González F G, Miralles D J, Slafer G A. 2011. Wheat floret survival as related to pre-anthesis spike growth. Journal of Experimental Botany, 62, 4889–4901.

Guo T, Chen K, Dong N Q, Ye W W, Shan J X, Lin H X. 2020. Tillering and small grain 1 dominates the tryptophan aminotransferase family required for local auxin biosynthesis in rice. Journal of Integrative Plant Biology, 62, 581–600.

Guo Z F, Chen D J, Schnurbusch T. 2018. Plant and floret growth at distinct developmental stages during the stem elongation phase in wheat. Frontiers in Plant Science, 9, 1–14.

Harb A, Krishnan A, Ambavaram M M, Pereira A. 2010. Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiology, 154, 1254–1271.

Herrera J, Calderini D F. 2020. Pericarp growth dynamics associate with final grain weight in wheat under contrasting plant densities and increased night temperature. Annals of Botany, 126, 1063–1076.

Hou J F, Huang X, Sun W, Du C Y, Wang C Y, Xie Y X, Ma Y. 2018. Accumulation of water-soluble carbohydrates and gene expression in wheat stems correlates with drought resistance. Journal of Plant Physiology, 231, 182–191.

Kaur H, Ozga J A, Reinecke D M. 2021. Balancing of hormonal biosynthesis and catabolism pathways, a strategy to ameliorate the negative effects of heat stress on reproductive growth. Plant, Cell & Environment, 44, 1486–1503.

Liang Z M, Cao X D, Gao R, Guo N, Tang Y Y, Vinay N, Liu Y. 2025. Brassinosteroids alleviates wheat floret degeneration under low nitrogen stress by promoting the distribution of sucrose from stems to spikes. Journal of Integrative Agriculture, doi.org/10.1016/j.jia.2023.12.017.

Lindström L I, Pellegrini C N, Aguirrezábal L A N, Herna´ndez L F. 2006. Growth and development of sunflower fruits under shade during pre and early post-anthesis period. Field Crops Research, 96, 151–159.

Liu C, Mao B G, Yuan D Y, Chu C C, Duan M J. 2021. Salt tolerance in rice: Physiological responses and molecular mechanisms. The Crop Journal, 10, 13–25.

Liu Y, Wang Q S, Ding Y F, Li G H, Xu J X, Wang S H. 2011. Effects of external ABA, GA3 and NAA on the tiller bud outgrowth of rice is related to changes in endogenous hormones. Plant Growth Regulation, 65, 247–254.

Liu Y H, Offler C E, Ruan Y L. 2016. Cell wall invertase promotes fruit set under heat stress by suppressing ROS-independent cell death. Plant Physiology, 172, 163–180.

Lizana X C, Riegel R, Gomez L D, Herrera J, Isla A, McQueen-Mason S J, Calderini D F. 2010. Expansins expression is associated with grain size dynamics in wheat (Triticum aestivum L.). Journal of Experimental Botany, 61, 1147–1157.

Meitzel T, Radchuk R, McAdam E L, Thormählen I, Feil R, Munz E, Hilo A, Geigenberger P, Ross J J, Lunn J E, Borisjuk L. 2021. Trehalose 6-phosphate promotes seed filling by activating auxin biosynthesis. New Phytologist, 229, 1553–1565.

Mena-Alí J I, Rocha O J. 2005. Selective seed abortion affects the performance of the offspring in Bauhinia ungulata. Annals of Botany, 95, 1017–1023.

O’Hara L E, Paul M J, Wingler A. 2013. How do sugars regulate plant growth and development? New insight into the role of trehalose-6-phosphate. Molecular Plant, 6, 261–274.

Ranwala A P, Miller W B. 1998. Sucrose-cleaving enzymes and carbohydrate pools in lilium longiflorum floral organs. Physiologia Plantarum, 103, 541–550.

Ruan Y, Patrick J W, Bouzayen M, Osorio S, Fernie A R. 2012. Molecular regulation of seed and fruit set. Trends in Plant Science, 17, 656–665.

Ruan Y L. 2014. Sucrose metabolism: Gateway to diverse carbon use and sugar signaling. Annual Review of Plant Biology, 65, 33–67.

Sah S K, Reddy K R, Li J X. 2016. Abscisic acid and abiotic stress tolerance in crop plants. Frontiers in Plant Science, 7, 571.

Schnyder H, Gillenberg C, Hinz J. 1993. Fructan contents and dry matter deposition in different tissues of the wheat grain during development. Plant, Cell & Environment, 16, 179–187.

Serrago R A I. Savin A R, Slafer G A. 2013. Understanding grain yield responses to source–sink ratios during grain filling in wheat and barley under contrasting environments. Field Crops Research, 150, 42–51.

Shen S, Liang X G, Zhang L, Zhao X, Liu Y P, Lin S, Gao Z, Wang P, Wang Z M, Zhou S L. 2020. Intervening in sibling competition for assimilates by controlled pollination prevents seed abortion under postpollination drought in maize. Plant, Cell & Environment, 43, 903–919.

Shen S, Zhang L, Liang X G, Zhao X, Lin S, Qu L H, Liu Y P, Gao Z, Ruan Y L, Zhou S L. 2018. Delayed pollination and low availability of assimilates are major factors causing maize kernel abortion. Journal of Experimental Botany, 69, 1599–1613.

Tang T, Xie H, Wang Y, Lü B, Liang J S. 2009. The Effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.). Journal of Experimental Botany, 60, 2641–2652.

Teng Z N, Yu H H, Wang G Q, Meng S, Liu B H, Yi Y K, Chen Y K, Zheng Q, Liu L, Yang J C, Duan M J, Zhang J H, Ye N H. 2021. Synergistic interaction between ABA and IAA due to moderate soil drying promotes grain filling of inferior spikelets in rice. The Plant Journal, 109, 1457–1472

Waadt R. 2020. Phytohormone signaling mechanisms and genetic methods for their modulation and detection. Current Opinion in Plant Biology, 57, 31–40.

Waddington S R, Cartwright P M, Wall P C. 1983. A quantitative scale of spike initial and pistil development in barley and wheat. Annals of Botany, 51, 119–130.

Wang G Q, Li H X, Feng L, Chen M X, Meng S, Ye N H, Zhang J H. 2019. Transcriptomic analysis of grain filling in rice inferior grains under moderate soil drying. Journal of Experimental Botany, 70, 1597–1611.

Wang G Q, Zhang J H. 2020. Carbohydrate, hormone and enzyme regulations of rice grain filling under post-anthesis soil drying. Environmental and Experimental Botany, 178, 104165.

Wang L L, Lu Q Q, Wen X X, Lu C C. 2015. Enhanced sucrose loading improves rice yield by increasing grain size. Plant Physiology, 169, 2848–2862.

Wang Z Q, Xu Y J, Chen T T, Zhang H, Yang J C, Zhang J H. 2015. Abscisic acid and the key enzymes and genes in sucrose-to-starch conversion in rice spikelets in response to soil drying during grain filling. Planta, 241, 1091–1107.

Wardlaw I F, Willenbrink J. 2000. Mobilization of fructan reserves and changes in enzyme activities in wheat stems correlate with water stress during kernel filling. New Phytologist, 148, 413–422.

Xie Q, Mayes S, Sparkes D L. 2015. Carpel size, grain filling, and morphology determine individual grain weight in wheat. Journal of Experimental Botany, 66, 6715–6730.

Xu X Y, E Z G, Zhang D P, Yun Q B, Zhou Y, Niu B X. Chen C. 2021. OsYUC11-mediated auxin biosynthesis is essential for endosperm development of rice. Plant Physiology, 185, 934–950.

Yadav U P, Ivakov A, Feil R, Duan G Y, Walther D, Giavalisco P, Piques M, Carillo P, Hubberten H M, Stitt M, Lunn J E. 2014. The sucrose-trehalose 6-phosphate (Tre6P) nexus: Specificity and mechanisms of sucrose signalling by Tre6P. Journal of Experimental Botany, 65, 1051–1068.

Yang J C, Zhang J H. 2006. Grain filling of cereals under soil drying. New Phytologist, 169, 223–236.

Yang J C, Zhang J H, Liu K, Wang Z Q, Liu L J. 2007. Abscisic acid and ethylene interact in rice spikelets in response to water stress during meiosis. Journal of Plant Growth Regulation, 26, 318–328.

Yang J C, Zhang J H, Wang Z Q, Xu G W, Zhu Q S. 2004. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiology, 135, 1621–1629.

Yang J C, Zhang J H, Wang Z Q, Zhu Q S. 2001. Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling. Journal of Experimental Botany, 52, 2169–2179.

Zadoks J C, Chang T T, Konzak C F. 1974. A decimal code for the growth stages of cereals. Weed Research, 14, 415–421.

Zhang H, Li H W, Yuan L M, Wang Z Q, Yang J C, Zhang J H. 2012. Post-anthesis alternate wetting and moderate soil drying enhances activities of key enzymes in sucrose-to-starch conversion in inferior spikelets of rice. Journal of Experimental Botany, 63, 215–227.

Zhang L, Liang X G, Shen S, Yin H, Zhou L L, Gao Z, Lv X Y, Zhou S L. 2018. Increasing the abscisic acid level in maize grains induces precocious maturation by accelerating grain filling and dehydration. Plant Growth Regulation, 86, 65–79.

Zhang M, Gao Y M, Zhang Z, Liu Y, Han M K, Hu N Y, Wang Z M, Sun Z C, Zhang Y H. 2020. Limited irrigation influence on rotation yield, water use, and wheat traits. Agronomy Journal, 112, 241–256.

Zhang X Y, Chen S Y, Sun H Y, Wang Y M, Shao L W. 2010. Water use efficiency and associated traits in winter wheat cultivars in the North China Plain. Agricultural Water Management, 97, 1117–1125.

Zhang Z, Huang J, Gao Y M, Liu Y, Li J P, Zhou X N, Yao C S, Wang Z M, Sun Z C, Zhang Y H. 2020. Suppressed ABA signal transduction in the spike promotes sucrose use in the stem and reduces grain number in wheat under water stress. Journal of Experimental Botany, 71, 7241–7256.

Zhang Z, Li J, Hu N Y, Li W, Qin W L, Li J P, Gao Y M, Liu Y, Sun Z C, Yu K, Wang Z M, Zhang Y H. 2021. Spike growth affects spike fertility through the number of florets with green anthers before floret abortion in wheat. Field Crops Research, 260, 108007.

Zhao Y. 2018. Essential roles of local auxin biosynthesis in plant development and in adaptation to environmental changes. Annual Review of Plant Biology, 69, 417–435.

Zinselmeier C, Jeong B R, Boyer J S. 1999. Starch and the control of kernel number in maize at low water potentials. Plant Physiology, 121, 25–36.

[1]	Abdoul Kader Mounkaila Hamani, Sunusi Amin Abubakar, Yuanyuan Fu, Djifa Fidele Kpalari, Guangshuai Wang, Aiwang Duan, Yang Gao, Xiaotang Ju. The coupled effects of various irrigation schedules and split nitrogen fertilization modes on post-anthesis grain weight variation, yield, and grain quality of drip-irrigated winter wheat (Triticum aestivum L.) in the North China Plain[J]. >Journal of Integrative Agriculture, 2025, 24(6): 2123-2137.
[2]	Xinyi Mao, Xuan Zhao, Zhi Luo, Ao He, Meng Yang, Mengjun Liu, Jin Zhao, Ping Liu. Transcriptome-based analysis of lignin accumulation in the regulation of fruit stone development and endocarp hardening in Chinese jujube[J]. >Journal of Integrative Agriculture, 2025, 24(6): 2217-2228.
[3]	Teame Gereziher Mehari, Marijana Skorić, Hui Fang, Kai Wang, Fang Liu, Tesfay Araya, Branislav Šiler, Dengbing Yao, Baohua Wang. *Insights into the role of GhCYP* and GhTPS in the gossypol biosynthesis pathway via a multiomics and functional-based approach in cotton**[J]. >Journal of Integrative Agriculture, 2025, 24(5): 1671-1687.
[4]	Xiaochun Wei, Yuanlin Zhang, Yanyan Zhao, Weiwei Chen, Ujjal Kumar Nath, Shuangjuan Yang, Henan Su, Zhiyong Wang, Wenjing Zhang, Baoming Tian, Fang Wei, Yuxiang Yuan, Xiaowei Zhang. *Mitotic pollen abnormalities are linked to Ogura cytoplasmic* male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis)**[J]. >Journal of Integrative Agriculture, 2025, 24(3): 1092-1107.
[5]	Zimeng Liang, Xidan Cao, Rong Gao, Nian Guo, Yangyang Tang, Vinay Nangia, Yang Liu. Brassinosteroids alleviate wheat floret degeneration under low nitrogen stress by promoting the redistribution of sucrose from stems to spikes[J]. >Journal of Integrative Agriculture, 2025, 24(2): 497-516.
[6]	Xiuling Wang, Li Niu, Huaipan Liu, Xucun Jia, Yulong Zhao, Qun Wang, Yali Zhao, Pengfei Dong, Moubiao Zhang, Hongping Li, Panpan An, Zhi Li, Xiaohuan Mu, Yongen Zhang, Chaohai Li. Integrated transcriptomics and metabolomics analysis provide insights into the alleviation of waterlogging stress in maize by exogenous spermidine application[J]. >Journal of Integrative Agriculture, 2025, 24(12): 4546-4560.
[7]	Dongming Liu, Jinfang Liang, Quanquan Liu, Yaxin Chen, Shixiang Duan, Dongling Sun, Huayu Zhu, Junling Dou, Huanhuan Niu, Sen Yang, Shouru Sun, Jianbin Hu, Luming Yang. *The pseudo-type response regulator gene Clsc* regulates rind stripe coloration in watermelon**[J]. >Journal of Integrative Agriculture, 2025, 24(1): 147-160.
[8]	Lijiao Ge, Weihao Miao, Kuolin Duan, Tong Sun, Xinyan Fang, Zhiyong Guan, Jiafu Jiang, Sumei Chen, Weimin Fang, Fadi Chen, Shuang Zhao. Comparative transcriptome analysis identifies key regulators of nitrogen use efficiency in chrysanthemum[J]. >Journal of Integrative Agriculture, 2025, 24(1): 176-195.
[9]	Meixue Sun, Tong Li, Yingjie Liu, Kenneth Wilson, Xingyu Chen, Robert I. Graham, Xianming Yang, Guangwei Ren, Pengjun Xu. *A dicistrovirus increases pupal mortality in Spodoptera* frugiperda by suppressing protease activity and inhibiting larval diet consumption**[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2723-2734.
[10]	Qian Wang, Huimin Cao, Jingcheng Wang, Zirong Gu, Qiuyun Lin, Zeyan Zhang, Xueying Zhao, Wei Gao, Huijun Zhu, Hubin Yan, Jianjun Yan, Qingting Hao, Yaowen Zhang. *Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean (Vigna* radiata L.)**[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2571-2588.
[11]	Hengwei Yu, Zhimei Yang, Jianfang Wang, Huaxuan Li, Xuefeng Li, Entang Liang, Chugang Mei, Linsen Zan. Identification of key genes and metabolites involved in meat quality performance in Qinchuan cattle by WGCNA[J]. >Journal of Integrative Agriculture, 2024, 23(11): 3923-3937.
[12]	Simin Liao, Zhibin Xu, Xiaoli Fan, Qiang Zhou, Xiaofeng Liu, Cheng Jiang, Liangen Chen, Dian Lin, Bo Feng, Tao Wang. *Genetic dissection and validation of a major QTL for grain weight on chromosome 3B in bread wheat (Triticum* aestivum L.)** [J]. >Journal of Integrative Agriculture, 2024, 23(1): 77-92.
[13]	CHU Jin-peng, GUO Xin-hu, ZHENG Fei-na, ZHANG Xiu, DAI Xing-long, HE Ming-rong. Effect of delayed sowing on grain number, grain weight, and protein concentration of wheat grains at specific positions within spikes[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2359-2369.
[14]	Zaid CHACHAR, Siffat Ullah KHAN, ZHANG Xue-huan, LENG Peng-fei, ZONG Na, ZHAO Jun. Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development[J]. >Journal of Integrative Agriculture, 2023, 22(2): 389-399.
[15]	LIU Yun-chuan, WANG Xiao-lu, HAO Chen-yang, IRSHAD Ahsan, LI Tian, LIU Hong-xia, HOU Jian, ZHANG Xue-yong. TaABI19 positively regulates grain development in wheat[J]. >Journal of Integrative Agriculture, 2023, 22(1): 41-51.

No Suggested Reading articles found!

Viewed

Full text

Abstract

Cited

Shared

Discussed

Cite this article:

About JIA

Editorial board

For authors