小麦是世界上主要的粮食作物之一。为满足日益增长的人口对小麦的需求的增长，实现小麦等作物的产量最大化，挖掘并阐明增产基因的功能为基因工程育种提供基础显得尤为重要。来自玉米的bHLH转录因子ABP7参与调控籽粒发育，本文通过转基因小麦，研究了ABP7对小麦产量相关性状的影响。分子鉴定结果表明，HB123 和 HB287转基因系基因组中存在多拷贝ABP7基因插入，并且其具有较高表达量，而转基因系QB205为单拷贝插入同时其表达量与野生型材料无显著差异。田间试验表型分析表明，HB123 和 HB287转基因系的穗粒数、穗粒重、千粒重、粒长和粒宽等籽粒产量相关性状以及小区产量都得到了提高，而QB205并没有在这些性状上与野生型表现出差异。此外，HB123 和 HB287转基因系的旗叶中的总叶绿素、叶绿素a和叶绿素b及总可溶性糖的含量相比于野生型材料都显著提高。总之，ABP7能够正向调控转基因小麦的籽粒相关性状并提高小区产量，可用于小麦增产育种。

Wheat is one of the major food crops in the world.  Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population.  This study investigated the role of ABP7, a bHLH transcription factor from maize involved in kernel development, in regulating grain yield-related traits in transgenic wheat.  Molecular characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene expression.  At the same time, QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type (WT) plants.  Phenotyping under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits (e.g., grain number per spike, grain weight per spike, thousand-grain weight, grain length, and grain width) and increased grain yield per plot, compared to WT plants, whereas line QB205 did not.  In addition, total chlorophyll, chlorophyll a, chlorophyll b, and total soluble sugars were largely increased in the flag leaves of both HB123 and HB287 transgenic lines compared to the WT.  These results strongly suggest that ABP7 positively regulates yield-related traits and plot grain yield in transgenic wheat.  Consequently, ABP7 can be utilized in wheat breeding for grain yield improvement