Please wait a minute...
Journal of Integrative Agriculture  2023, Vol. 22 Issue (2): 389-399    DOI: 10.1016/j.jia.2022.08.052
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development
Zaid CHACHAR, Siffat Ullah KHAN, ZHANG Xu-huan, LENG Peng-fei, ZONG Na, ZHAO Jun
Faculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      

小麦是世界上主要的粮食作物之一。为满足日益增长的人口对小麦的需求的增长,实现小麦等作物的产量最大化,挖掘并阐明增产基因的功能为基因工程育种提供基础显得尤为重要。来自玉米的bHLH转录因子ABP7参与调控籽粒发育,本文通过转基因小麦,研究了ABP7对小麦产量相关性状的影响。分子鉴定结果表明,HB123 和 HB287转基因系基因组中存在多拷贝ABP7基因插入,并且其具有较高表达量,而转基因系QB205为单拷贝插入同时其表达量与野生型材料无显著差异。田间试验表型分析表明,HB123 和 HB287转基因系的穗粒数、穗粒重、千粒重、粒长和粒宽等籽粒产量相关性状以及小区产量都得到了提高,而QB205并没有在这些性状上与野生型表现出差异。此外,HB123 和 HB287转基因系的旗叶中的总叶绿素、叶绿素a和叶绿素b及总可溶性糖的含量相比于野生型材料都显著提高。总之,ABP7能够正向调控转基因小麦的籽粒相关性状并提高小区产量,可用于小麦增产育种。


Wheat is one of the major food crops in the world.  Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population.  This study investigated the role of ABP7, a bHLH transcription factor from maize involved in kernel development, in regulating grain yield-related traits in transgenic wheat.  Molecular characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene expression.  At the same time, QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type (WT) plants.  Phenotyping under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits (e.g., grain number per spike, grain weight per spike, thousand-grain weight, grain length, and grain width) and increased grain yield per plot, compared to WT plants, whereas line QB205 did not.  In addition, total chlorophyll, chlorophyll a, chlorophyll b, and total soluble sugars were largely increased in the flag leaves of both HB123 and HB287 transgenic lines compared to the WT.  These results strongly suggest that ABP7 positively regulates yield-related traits and plot grain yield in transgenic wheat.  Consequently, ABP7 can be utilized in wheat breeding for grain yield improvement

Keywords:  transgenic wheat       ABP7        kernel development       grain weight       grain width  
Received: 24 August 2021   Accepted: 27 December 2021

About author:  Zaid CHACHAR, E-mail:; Correspondence ZHAO Jun, Tel: +86-10-82105320, E-mail:; ZONG Na, Tel: +86-10-82106139, E-mail:

Cite this article: 

Zaid CHACHAR, Siffat Ullah KHAN, ZHANG Xue-huan, LENG Peng-fei, ZONG Na, ZHAO Jun. 2023. Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development. Journal of Integrative Agriculture, 22(2): 389-399.

Araus J L, Serret M D, Lopes M S. 2019. Transgenic solutions to increase yield and stability in wheat: shining hope or flash in the pan? Journal of Experimental Botany, 70, 1419–1424.
Arnon D I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1–15.
Cao R, Guo L, Ma M, Zhang W, Liu X, Zhao H. 2019. Identification and functional characterization of Squamosa promoter binding protein-like gene TaSPL16 in wheat (Triticum aestivum L.). Frontiers in Plant Science, 10, 212–224.
Doyle J J, Doyle J L, Brown A H, Grace J P. 1990. Multiple origins of polyploids in the Glycine tabacina complex inferred from chloroplast DNA polymorphism. Proceedings of the National Academy of Sciences of the United States of America, 87, 714–717.
Lafiandra D, Riccardi G, Shewry  P R. 2014. Improving cereal grain carbohydrates for diet and health. Journal of Cereal Science, 59, 312–326.
Langridge P. 2013. Wheat genomics and the ambitious targets for future wheat production. Genome, 56, 545–547.
Lastdrager J, Hanson J, Smeekens S. 2014. Sugar signals and the control of plant growth and development. Journal of Experimental Botany, 65, 799–807.
Li N, Li Y. 2016. Signaling pathways of seed size control in plants. Current Opinion in Plant Biology, 33, 23–32.
Li P, Wu P, Chen J. 2012. Evaluation of flag leaf chlorophyll content index in 30 spring wheat genotypes under three irrigation regimes. Australian Journal of Crop Science, 6, 1123–1130.
Li Y, He N, Hou J, Xu L, Liu C, Zhang J, Wang Q, Zhang X, Wu X. 2018. Factors influencing leaf chlorophyll content in natural forests at the biome scale. Frontiers in Ecology and Evolution, 6, 64–73.
Liu H, Li H, Hao C, Wang K, Wang Y, Qin L, An D, Li T, Zhang X. 2020. TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.). Plant Biotechnology Journal, 18, 1330–1342.
Ma L, Li T, Hao C, Wang Y, Chen X, Zhang X. 2016. TaGS5-3A, a grain size gene selected during wheat improvement for larger kernel and yield. Plant Biotechnology Journal, 14, 1269–1280.
Nadolska-Orczyk A, Rajchel I K, Orczyk W, Gasparis S. 2017. Major genes determining yield-related traits in wheat and barley. Theoretical and Applied Genetics, 130, 1081–1098.
Porra R J, Thompson W A, Kriedemann P E. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (Bioenergetics), 975, 384–394.
Reif J C, Zhang P, Dreisigacker S, Warburton M L, van Ginkel M, Hoisington D, Bohn M, Melchinger A E. 2005. Wheat genetic diversity trends during domestication and breeding. Theoretical and Applied Genetics, 110, 859–864.
Saalbach I, Mora-Ramírez I, Weichert N, Andersch F, Guild G, Wieser H, Koehler P, Stangoulis J, Kumlehn J, Weschke W, Weber H. 2014. Increased grain yield and micronutrient concentration in transgenic winter wheat by ectopic expression of a barley sucrose transporter. Journal of Cereal Science, 60, 75–81.
Shewry  P R, Hey  S J. 2015. The contribution of wheat to human diet and health. Food and Energy Security, 4, 178–202.
Simmonds J, Scott P, Brinton J, Mestre T C, Bush M, Del Blanco A, Dubcovsky J, Uauy C. 2016. A splice acceptor site mutation in TaGW2-A1 increases thousand grain weight in tetraploid and hexaploid wheat through wider and longer grains. Theoretical and Applied Genetics, 129, 1099–1112.
Smidansky E D, Clancy M, Meyer F D, Lanning S P, Blake N K, Talbert L E, Giroux M J. 2002. Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield. Proceedings of the National Academy of Sciences of the United States of America, 99, 1724–1729.
Su Z, Hao C, Wang L, Dong Y, Zhang X. 2011. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 122, 211–223.
Wang S, Zhang X, Chen F, Cui D. 2015. A single-nucleotide polymorphism of TaGS5 gene revealed its association with kernel weight in Chinese bread wheat. Frontiers in Plant Science, 6, 1166–1175.
Wang W, Pan Q, Tian B, He F, Chen Y, Bai G, Akhunova A, Trick H N, Akhunov E. 2019. Gene editing of the wheat homologs of TONNEAU 1-recruiting motif encoding gene affects grain shape and weight in wheat. The Plant Journal, 100, 251–264.
Yadav D, Shavrukov, Y, Bazanova N, Chirkova L, Borisjuk N, Kovalchuk N, Ismagul A, Parent B, Langridge P, Hrmova M, Lopato S. 2015. Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield. Journal of Experimental Botany, 66, 6635–6650.
Yan X, Zhao L, Ren Y, Dong Z, Cui D, Chen F. 2019. Genome-wide association study revealed that the TaGW8 gene was associated with kernel size in Chinese bread wheat. Scientific Reports, 9, 1–10.
Zhang K, Zhang Y, Chen G, Tian J. 2009. Genetic analysis of grain yield and leaf chlorophyll content in common wheat. Cereal Research Communications, 37, 499–511.
Zhang X, Deng Z, Wang Y, Li J, Tian J. 2014. Unconditional and conditional QTL analysis of kernel weight related traits in wheat (Triticum aestivum L.) in multiple genetic backgrounds. Genetica, 142, 371–379.

[1] CHU Jin-peng, GUO Xin-hu, ZHENG Fei-na, ZHANG Xiu, DAI Xing-long, HE Ming-rong. Effect of delayed sowing on grain number, grain weight, and protein concentration of wheat grains at specific positions within spikes[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2359-2369.
[2] LIU Yun-chuan, WANG Xiao-lu, HAO Chen-yang, IRSHAD Ahsan, LI Tian, LIU Hong-xia, HOU Jian, ZHANG Xue-yong. TaABI19 positively regulates grain development in wheat[J]. >Journal of Integrative Agriculture, 2023, 22(1): 41-51.
[3] WU Xiao-li, LIU Miao, LI Chao-su, Allen David (Jack) MCHUGH, LI Ming, XIONG Tao, LIU Yu-bin, TANG Yong-lu. Source–sink relations and responses to sink–source manipulations during grain filling in wheat[J]. >Journal of Integrative Agriculture, 2022, 21(6): 1593-1605.
[4] XUE Pao1, ZHANG Ying-xin1, LOU Xiang-yang1, ZHU Ai-ke, CHEN Yu-yu, SUN Bin, YU Ping, CHENG Shi-hua, CAO Li-yong, ZHAN Xiao-deng .
Mapping and genetic validation of a grain size QTL qGS7.1 in rice (Oryza sativa L.)
[J]. >Journal of Integrative Agriculture, 2019, 18(8): 1838-1850.
[5] WANG Yi-xue, XU Qiao-fang, CHANG Xiao-ping, HAO Chen-yang, LI Run-zhi, JING Rui-lian. A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat[J]. >Journal of Integrative Agriculture, 2018, 17(2): 276-284.
[6] GAO Fang-yuan, ZENG Li-hua, QIU Ling, LU Xian-jun, REN Juan-sheng, WU Xian-ting, SU Xiangwen, GAO Yong-ming, REN Guang-jun. QTL mapping of grain appearance quality traits and grain weight using a recombinant inbred population in rice (Oryza sativa L.)[J]. >Journal of Integrative Agriculture, 2016, 15(8): 1693-1702.
[7] PENG Ting, Lü Qiang, ZHAO Ya-fan, SUN Hong-zheng, HAN Ying-chun, DU Yan-xiu, ZHANG Jing, LI Jun-zhou, WANG Lin-lin, ZHAO Quan-zhi. Superior grains determined by grain weight are not fully correlated with the flowering order in rice[J]. >Journal of Integrative Agriculture, 2015, 14(5): 847-855.
[8] CHANG Jian-zhong, HAO Chen-yang, CHANG Xiao-ping, ZHANG Xue-yong , JING Rui-lian. HapIII of TaSAP1-A1, a Positively Selected Haplotype in Wheat Breeding[J]. >Journal of Integrative Agriculture, 2014, 13(7): 1462-1468.
[9] XU Man-yu, ZHOU Ting, ZHAO Yan-ying, LI Jia-bao, XU Heng, DONG Han-song , ZHANG Chun-ling. Transgenic Expression of a Functional Fragment of Harpin Protein Hpa1 in Wheat Represses English Grain Aphid Infestation[J]. >Journal of Integrative Agriculture, 2014, 13(12): 2565-2576.
[10] ZHANG Qiang, YAO Guo-xin, HU Guang-long, TANG Bo, ZHANG Hong-liang, LI Zi-chao . Fine Mapping of qTGW3-1, a QTL for 1000-Grain Weight on Chromosome 3 in Rice[J]. >Journal of Integrative Agriculture, 2012, 12(6): 879-887.
No Suggested Reading articles found!