麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
|Identification and validation of novel loci associated with wheat quality through a genome-wide association study
PU Zhi-en1*, YE Xue-ling3, 4*, LI Yang1, SHI Bing-xin1, GUO Zhu1, DAI Shou-fen2, MA Jian2, LIU Ze-hou4, JIANG Yun-feng2, LI Wei1, JIANG Qian-tao2, CHEN Guo-yue2, WEI Yu-ming2, ZHENG You-liang2
1 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, P.R.China
3 Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs/College of Food and Biological Engineering, Chengdu University, Chengdu 610106, P.R.China
4 Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
摘要 了解小麦品质相关性状的遗传基础有助于对小麦品质进行改良，本实验测定了多环境下236份小麦种质资源（包括 160 个栽培品种和76个地方品种）的蛋白质含量（GPC）、淀粉含量（GSC）和湿面筋含量（WGC），并使用 55K小麦芯片进行了混合线性模型 (MLM)分析。结果共鉴定了 12 个稳定的 QTL/SNP，与GPC、GSC和WGC 相关的位点分别有3个、7个和2个 QTL，它们分别位于1B、1D、2A、2B、2D、3B、3D、5D 和 7D 染色体上；表型变异解释 (PVE) 范围从4.2 至10.7%。与之前报道的 QTL/基因相比，5 个 QTL（QGsc.sicau-1BL、QGsc.sicau-1DS、QGsc.sicau-2DL.1、QGsc.sicau-2DL.2、QWgc.sicau-5DL）是潜在的新位点。本实验着重关注了位于5D染色体上与湿面筋浓度相关的稳定QTL，并成功开发了SNP AX-108770574 和AX-108791420 两个KASP 标记。其中AX-108770574中含有A-等位基因和AX-108791420中含有T-等位基因的品种表型显着高于(P<0.01)含有湿面筋浓度G-等位基因或C-等位基因的地方品种，表明开发的KASP 标记可用于分子育种，改良小麦品质。
Abstract Understanding the genetic basis of quality-related traits contributes to the improvement of grain protein concentration (GPC), grain starch concentration (GSC), and wet gluten concentration (WGC) in wheat, a genome-wide association study (GWAS) based on a mixed linear model (MLM) was performed on the 236 wheat accessions including 160 cultivars and 76 landraces using 55K single nucleotide polymorphism (SNP) array in multiple environments. A total of twelve stable QTL/SNPs were identified to control different quality traits in this populations at least two environments under stripe rust stress; three, seven and two QTLs associated with GPC, GSC, and WGC were characterized respectively and located on chromosomes 1B, 1D, 2A, 2B, 2D, 3B, 3D, 5D, and 7D with the range of phenotypic variation explained (PVE) from 4.2 to 10.7%. Compared with the previously reported QTLs/genes, five QTLs (QGsc.sicau-1BL, QGsc.sicau-1DS, QGsc.sicau-2DL.1, QGsc.sicau-2DL.2, QWgc.sicau-5DL) were potentially novel. KASP markers for SNPs AX-108770574 and AX-108791420 on chromosome on 5D associated with wet gluten concentration were successfully developed. Phenotype of the cultivars containing the A-allele in AX-108770574 and T-allele in AX-108791420 were extremely significantly (P<0.01) higher than that of the landraces containing the G-allele or C-allele of wet gluten concentration in each of the environments. The developed and validated KASP markers could be utilized in molecular breeding aiming to improve the quality in wheat.
Received: 25 April 2021
Accepted: 01 July 2021
|Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0100900, 2016YFD0102000 and 2016YFD0100100), the International Science and Technology Cooperation and Exchanges Programs of Science and Technology Department of Sichuan Province, China (2019YFH0063), and the Sichuan Science and Technology Program, China (2022ZDZX0014).
|About author: Correspondence ZHENG You-liang, Tel/Fax: +86-28-86290909, E-mail: firstname.lastname@example.org
* These authors contributed equally to this study.
Cite this article:
PU Zhi-en, YE Xue-ling, LI Yang, SHI Bing-xin, GUO Zhu, DAI Shou-fen, MA Jian, LIU Ze-hou, JIANG Yun-feng, LI Wei, JIANG Qian-tao, CHEN Guo-yue, WEI Yu-ming, ZHENG You-liang.
Identification and validation of novel loci associated with wheat quality through a genome-wide association study. Journal of Integrative Agriculture, 21(11): 3131-3147.
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