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Journal of Integrative Agriculture  2021, Vol. 20 Issue (10): 2592-2600    DOI: 10.1016/S2095-3119(20)63324-X
Special Issue: 水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
A rice geranylgeranyl reductase is essential for chloroplast development
LIU Xi, YI Xin, YANG Yan-rong, HUANG Qian-qian
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huai’an 223300, P.R.China
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Abstract  Chloroplasts are essential for plant photosynthesis and growth. Many genes have been identified that regulate plant chloroplast development. However, it is not known at a molecular level how these genes regulate chloroplast biogenesis. In this study, we isolated a mutant ygl2 (yellow-green leaf2) that exhibited a pigment-defective phenotype. YGL2 encodes a geranylgeranyl reductase, and in mutant ygl2, there was a single base change (T1361G) located in the third exon of YGL2 that resulted in a missense mutation (L454R) in the encoded product. Transmission electron microscopy revealed that chloroplast development was impaired in the ygl2 mutant. The expression levels of plastid-encoded genes were significantly altered in the ygl2 mutant. Furthermore, in a yeast two-hybrid assay, we found that YGL2 interacted with the RNA editing factor MORF8.
Keywords:  rice (Oryza sativa L.)        chloroplast        YGL2        geranylgeranyl reductase  
Received: 07 February 2020   Accepted:
This work was supported by the National Natural Science Foundation of China (31900388), the Natural Science Foundation of Jiangsu Province, China (BK20191055) and the Natural Science Foundation of Colleges of Jiangsu Province, China (19KJB180009).
Corresponding Authors:  Correspondence LIU Xi, Tel: +86-517-83525991,   

Cite this article: 

LIU Xi, YI Xin, YANG Yan-rong, HUANG Qian-qian. 2021. A rice geranylgeranyl reductase is essential for chloroplast development. Journal of Integrative Agriculture, 20(10): 2592-2600.

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