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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 988-998    DOI: 10.1016/S2095-3119(19)62672-9
Special Issue: Rice Physiology · Biochemistry · Cultivation · Tillage
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Characterization of eating quality and starch properties of two Wx alleles japonica rice cultivars under different nitrogen treatments
HUANG Shuang-jie1, 2, ZHAO Chun-fang1, ZHU Zhen1, ZHOU Li-hui1, ZHENG Qing-huan3, WANG Cai-lin1
1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, P.R.China
2 College of Tea Science, Xinyang Agriculture and Forestry University, Xinyang 464000, P.R.China
3 Agri-Tech Extension Center of Wancheng District, Nanyang 473000, P.R.China
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Abstract  
To understand the effect of nitrogen (N) fertilizer on rice (Oryza sativa L.) eating and cooking quality (ECQ).  Here, we investigated the ECQ attributes, physicochemical foundation of ECQ, and amylopectin fine structure of two Waxy (Wx) alleles japonica rice cultivars Nanjing 9108 (NJ9108) and Huaidao 5 (HD5) under four N rates (0, 150, 300, and 450 kg ha–1).  Sensory and pasting properties of the two cultivars varied depending on N rates.  Compared with the control (0 kg ha–1), the overall eating quality and sensory value were significantly decreased under the N rates of 300 and 450 kg ha–1.  Further, conventional descriptive analysis showed that the stickiness and retrogradation of cooked rice were significantly decreased.  These results indicated that application of N fertilizer seems to affect the texture of cooked rice, causing it to be less sticky, lowering its retrogradation, and consequently reducing its palatability.  Results from rapid visco analyzer (RVA) revealed that the peak and breakdown viscosities significantly decreased, while the setback viscosity and peak time increased under the N rates of 300 and 450 kg ha–1.  However, no significant difference was observed when the N rate was 150 kg ha–1, indicating that less N fertilization can maintain rice ECQ.  As the N rate increasing, protein content increased, whereas apparent amylose content, starch content, and gel consistency almost unchanged.  Interestingly, compared with the control, under N treatments, the percentage of short amylopectin branches in NJ9108 was decreased, but increased in HD5, as controlled by amylopectin synthesis-related genes.  Notably, SSI and BEIIb were down-regulated in NJ9108, whereas BEIIb was up-regulated in HD5.  Thus, the palatability of both rice cultivars was significantly decreased under excessive N fertilization as a consequence of reduced stickiness and retrogradation of the cooked rice, which might have resulted from an elevated protein content and altered amylopectin fine structure.  In addition, amylopectin synthesis appeared to be affected by N fertilizer and the genotype of the rice cultivar.
 
Keywords:  rice (Oryza sativa L.)        nitrogen fertilizer        eating and cooking quality        amylopectin fine structure  
Received: 17 December 2018   Accepted: 04 March 2020
Fund: This work was funded by the China Postdoctoral Science Foundation (2017M611753), the earmarked fund for Agriculture Research System of China (CARS-01-62), the National Key R&D Program of China (2015BAD01B02), the Natural Science Foundation of Jiangsu Province, China (BK20180302), the Jiangsu Agriculture Science and Technology Innovation Fund, China (CX[17]3009), and the Project of Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan, China (HNKLTCU2018004).
Corresponding Authors:  Correspondence WANG Cai-lin, Tel: +86-25-84390317, E-mail: clwang@jaas.ac.cn    

Cite this article: 

HUANG Shuang-jie, ZHAO Chun-fang, ZHU Zhen, ZHOU Li-hui, ZHENG Qing-huan, WANG Cai-lin. 2020. Characterization of eating quality and starch properties of two Wx alleles japonica rice cultivars under different nitrogen treatments. Journal of Integrative Agriculture, 19(4): 988-998.

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