Please wait a minute...
Journal of Integrative Agriculture  2019, Vol. 18 Issue (7): 1529-1540    DOI: 10.1016/S2095-3119(19)62730-9
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Gene bank scheduling of seed regeneration: Interim report on a long term storage study
Robert Redden1, Debra Partington 
1 Australian Temperate Field Crops Collection, Private Bag 260, Agriculture Victoria, Horsham, Victoria 3401, Australia (Retired, formerly curator)
2 Agriculture Victoria, Hamilton 3300, Australia
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  
A major challenge for the management of gene banks is the maintenance of good seed health in the collections.  Large germplasm collections >10 000 accessions often have been acquired from different sources over a range of dates, may differ in germination at time of deposition in the gene bank, and may have genetic differences in seed longevity.  The major storage variables affecting seed longevity are temperature and seed moisture content.  Two varieties of each pea (Pisum sativus L.), lentil (Lens culinaris Medikus subsp. culinaris), and chickpea (Cicer arietinum L.), were stored at three temperatures; 40, 20, and 2°C, each with three seed moisture levels of 10.9–13.8% (high), 7.9–10.3% (medium), and 7–7.8% (low), in the Australian Temperate Field Crops Collection gene bank.  Seed longevity at a given storage period was estimated by the corresponding germination percentage for each treatment.  This paper is an interim report on seed viability decline in the first seven years of this seed longevity study, in which viability decline towards zero was almost completed in the three seed moisture treatments at 40°C and the 20°C high seed moisture treatment, but had not declined in the other treatments.  Seed longevity positively responded to a reduction in temperature and then to a reduction in seed moisture.  The number of days in storage for seed germination decline to 85% (p85), and to 50% (p50) for mean seed viability, are reported by storage/varietal treatment.  Both p85 and p50 showed significant inverse linear responses with seed moisture at 40°C for pea and lentil varieties, with intra-specific variation for pea.  This long term trial aims to provide informed timing of seed regeneration for accessions in a gene bank.
Keywords:  gene bank        storage        moisture level              temperature              regeneration        pea        lentil        chickpea  
Received: 23 February 2018   Accepted: Online: 11 July 2018  
Fund: This long term study was supported by the core budget for Australian Temperate Field Crops Collection (ATFCC)operations, jointly funded by the Government of Victoria and the Grains Research and Development Corporation of Australia.
Corresponding Authors:  Correspondence Robert Redden, Tel: +61-03-53810818, E-mail: bbredden@yahoo.com.au   

Cite this article: 

Robert Redden, Debra Partington. 2019. Gene bank scheduling of seed regeneration: Interim report on a long term storage study. Journal of Integrative Agriculture, 18(7): 1529-1540.

Dickie J B, Ellis R H, Kraak H L, Tompsett P B. 1990. Temperature and seed longevity. Annals of Botany, 65, 197–204.
Ellis R H. 1991. The longevity of seed. Horticultural Science, 26, 1119–1125.
Ellis R H, Roberts E H. 1980a. Improved equations for the prediction of seed longevity. Annals of Botany, 45, 13–30.
Ellis R H, Roberts E H. 1980b. The influence of temperature and seed moisture on seed viability period in barley (Hordeum distichum L.). Annals of Botany, 45, 31–47.
Ellis R H, Roberts E H. 1982. Desiccation, rehydration, germination, imbibition injury and longevity of pea seeds (Pisum sativum). Seed Science and Technology, 10, 501–508.
FAO (Food and Agriculture Organization of the United Nations). 2014. Chapter 4.3. Gene bank standards for seed viability monitoring. In: FAO Working Group, ed., Gene Bank Standards for Plant Genetic Resources for Food and Agriculture. Rev. ed. FAO, Rome. pp. 30–31.
Freitas R A, Dias D C F S, Oliviera G A, Dias A S, Jose I C. 2006. Physiological and biochemical changes in naturally and artificially aged cotton seeds. Seed Science and Technology, 34, 253–264.
Fu Y B, Ahmed Z, Diedrichsen A. 2015. Towards a better monitoring of seed ageing under ex-situ seed conservation. Conservation Biology, 3, 1–16.
Hay F. 2003. The seed viability equations. In: Millennium Seed Bank Project. Kew, UK.
Hay F R, de Guzman F, Ellis D, Makahiya H, Borromeo T, Sackville-Hamilton R N. 2013. Viability of Oryza sativa L. seed stored under gene bank conditions for up to 30 years. Genetic Resources and Crop Evolution, 60, 275–296.
Hay F R, de Guzman F, Sackville-Hamilton R N. 2015. Viability monitoring intervals for gene bank samples of Oryza sativa. Seed Science and Technology, 43, 215–237.
He C, Liu Y, Wu K, Yuan M, Feng Q, Liu Y, Yan G J, Rose I A, Redden R J, Enneking D. 2008. Collecting and surveying landraces of pea (Pisum sativum) and faba bean (Vicia faba) in Qinghai province of China. Plant Genetic Resources Newsletter, 156, 1–10.
van Hintum T H J L, van Treuren H. 2012. Reliability of germplasm testing of ex-situ conserved seeds: A gene bank study on outsourced analyses. Plant Genetic Resources Characterization and Utilization, 10, 134–136.
Hong T D, Linington S, Ellis R H. 1996. Seed storage behaviour: A compendium. In: Handbook for Gene Banks. No. 4. International Plant Genetic Resources Institute, Rome.
Hubbard J E, Earle F R, Senti F R. 1957. Moisture relations in wheat and corn. Cereal Chemistry, 34, 422–433.
Imolehin E D. 1983. Rice seed borne fungi and their effect on seed germination. Plant Disease, 67, 1334–1336.
ISTH (International Seed Testing Handbook). 2003. ISTA secretariat, Basserdorf, Switzerland.
Koo B, Pardey P G, Wright B D. 2003. The economic costs of conserving genetic resources at the CGIAR centres. Agricultural Economics, 29, 287–297.
Lawrence P. 1995. Australia: Regeneration of tropical field crop germplasm in Australia. In: Engels J M M, Ramanatha R, eds., Regeneration of Seed Crops and their Wild Relatives, Country Reports on Regeneration Practices and Problems. Proceedings of a Consultation Meeting, ICRISAT (International Crops Research Institute for the Semi-Arid Tropics), Hyderabad, India. pp. 5–7.
Lee H S, Lee Y Y, Jeon Y A, Kim Y G. 2013. Comparison of seed viability among 42 species stored in a gene bank. Korean Journal Crop Science, 58, 432–438.
Murariu D. 1966. Prediction of life duration of maize (Zea mays L.) seed, preserved in gene banks, by basic viability equations. Romanian Agricultural Research, 5–6, 69–73.
Nagel M, Arif M A R, Rosenhauer M, Börner A. 2009. Longevity of seeds - intraspecific differences in the Gatersleben genebank collections. Tagung der Vereingung der Pflanzenzuchter und Saatkaufleute Osterreichs, Raumberg-Gumpenstein. St. Polten, Austria. pp. 179–181.
Nagel M, Kranner I, Neumann K, Rolletschek H, Seal C E, Colville L, Fernandez-Martin B, Börner A. 2014. Genome-wide association mapping and biochemical markers reveal that seed ageing and longevity are intricately affected by genetic background and developmental and environmental conditions in barley. Plant Cell and Environment, 38, 1011–1022.
Nagel M, Rosenhauer M, Willner E, Snowden R J, Freidt W, Börner A. 2011. Seed longevity in oilseed rape (Brassica napus L.) - genetic variation and QTL mapping. Plant Genetic Resources: Characterisation and Utilization, 9, 260–263.
Perez-Garcia P, Gonzalez-Benito M E, Gomez-Campo C. 2007. High viability recorded in ultra-dry seeds of Brassiceae after almost 40 years of storage. Seed Science and Technology, 35, 143–153.
PGRFA (Plant Genetic Resources for Food and Agriculture) report. 2011. Draft Updated Gene Bank Standards: Minimum Standards for Conservation of Orthodox Seeds. CGRFA 13/11/9. Commission on Genetic Resources for Food and Agriculture, FAO, Rome. p. 40.
Rao N K, Hanson J, Dulloo M E, Ghosh K, Nowell D, Larinde M. 2006. Manual of seed handling in gene banks. In: Handbooks for Gene Banks No. 8. Bioversity International, Rome, Italy. p. 146.
Roberts E H. 1973. Predicting the storage life of seeds. Seed Science & Technology, 1, 499–514.
Roberts E H. 1982. Monitoring seed viability in gene banks. In: Dickie J B, Linington S, Williams J T, eds., Seed Management Techniques for Gene Banks. Proceedings of a Workshop. Royal Botanical Gardens, Kew. pp. 268–277.
Roberts E H, Abdalla F H. 1968. The influence of temperature, moisture and oxygen on period of seed viability in barley, broad beans and peas. Annals of Botany, 32, 97–117.
Roberts E H, Ellis R N. 1989. Water and seed survival. Annals of Botany, 63, 39–52.
Sackville Hamilton N R, Chorlton K H. 1997. Regeneration of accessions in seed collections: A decision guide. In: Engels J, ed., Handbook for Gene Banks No. 5. (7.2.1.1). International Plant Genetic Resources Institute, Rome. p. 72.
Sinicio R. 2004. Generalised longevity model for orthodox seeds. Biosystems Engineering, 89, 85–92.
Tang J, Sokhansanj S. 1990. Viability of lentil seeds during drying. American Society of Agricultural Engineers, 22, 19.
Trapp II A, Dixon P M, Widrlechner M P, Kovach D A. 2012. Scheduling viability tests for seeds in long-term storage based on a Bayesian multi-level model. Journal of Agricultural, Biological, and Environmental Statistics, 17, 192–208.
van Treuren R, de Groot E C, van Hintum T H J L. 2013. Preservation of seed viability during 25 years of storage under standard gene bank conditions. Genetic Resources and Crop Evolution, 60, 1407–1421.
Vertucci C W, Roos E E, Crane J. 1994. Theoretical basis of protocols for seed storage III. Optimum moisture contents for pea seeds stored at different temperatures. Annals of Botany, 74, 531–540.
Walters C, Wheeler L M, Grotenhuis J M. 2005. Longevity of seeds stored in a gene bank: Species characteristics. Seed Science Research, 15, 1–20.
Zhukova N V, Voluzneva T A. 1989. Viability of lentil seeds after storage and accelerated ageing. Nauchno-Tekhnicheskii Byulletin’ Vsesoyuznogo Ordena Lenina i Orderna Druzby Narodov Nauchno-Issledovatel’skogo Instituta Rastenievodstva Imeni N.I. Vavilova, 193, 49–54.
[1] Md. Zasim Uddin, Md. Nadim Mahamood, Ausrukona Ray, Md. Ileas Pramanik, Fady Alnajjar, Md Atiqur Rahman Ahad. E2ETCA: End-to-end training of CNN and attention ensembles for rice disease diagnosis[J]. >Journal of Integrative Agriculture, 2026, 25(2): 756-768.
[2] Jinbu Wang, Wencheng Zong, Liangyu Shi, Mianyan Li, Jia Li, Deming Ren, Fuping Zhao, Lixian Wang, Ligang Wang. Using mixed kernel support vector machine to improve the predictive accuracy of genome selection[J]. >Journal of Integrative Agriculture, 2026, 25(2): 775-787.
[3] Yaling Yu, Hongfan Ge, Hang Gao, Yanyan Zhang, Kangping Liu, Zhenlei Zhou. Changes of bone remodeling, cartilage damage and apoptosis-related pathways in broilers with femoral head necrosis[J]. >Journal of Integrative Agriculture, 2026, 25(2): 788-802.
[4] Hui Song, Meiran Li, Zhenquan Duan. Current status of the genetic transformation of Arachis plants[J]. >Journal of Integrative Agriculture, 2026, 25(2): 577-584.
[5] Yue Song, Heng Wang, Mingyang Wang, Yumin Wang, Xiuxiang Lu, Wenjie Fan, Chen Yao, Pengxiang Liu, Yanjie Ma, Shengli Ming, Mengdi Wang, Lijun Shi. A novel TLR7 agonist exhibits antiviral activity against pseudorabies virus[J]. >Journal of Integrative Agriculture, 2026, 25(2): 803-813.
[6] Qiuling Huang, Yan Liao, Chunhui Huang, Huan Peng, Lingchiu Tsang, Borong Lin, Deliang Peng, Jinling Liao, Kan Zhuo. Integrative identification of Aphelenchoides fragariae (Nematoda: Aphelenchoididae) parasitizing Fuchsia hybrid in China[J]. >Journal of Integrative Agriculture, 2026, 25(2): 769-774.
[7] Xijun Wang, Hong Huo, Lei Shuai, Jinying Ge, Liyan Peng, Jinming Wang, Shuang Xiao, Weiye Chen, Zhiyuan Wen, Jinliang Wang, Zhigao Bu. Evaluation of safety and immunogenicity of a genetically modified rabies virus for use as an oral vaccine in several non-target species[J]. >Journal of Integrative Agriculture, 2026, 25(2): 814-819.
[8] Jing Gao, Shenglan Li, Yi Lei, Qi Wang, Zili Ning, Zhaohong Lu, Xianming Tan, Mei Xu, Feng Yang, Wenyu Yang. Delayed photosynthesis response causes carbon assimilation reduction in soybean under fluctuating light[J]. >Journal of Integrative Agriculture, 2026, 25(2): 648-658.
[9] Jun Deng, Ke Liu, Xiangqian Feng, Jiayu Ye, Matthew Tom Harrison, Peter de Voil, Tajamul Hussain, Liying Huang, Xiaohai Tian, Meixue Zhou, Yunbo Zhang. Exploring strategies for agricultural sustainability in super hybrid rice using the food–carbon–nitrogen–water–energy–profit nexus framework[J]. >Journal of Integrative Agriculture, 2026, 25(2): 624-638.
[10] Lihong Ma, Pengtao Wang, QianHao Zhu, Xinqi Cheng, Tao Zhang, Xinyu Zhang, Huaguo Zhu, Zuoren Yang, Jie Sun, Feng Liu. Unbalanced lipid metabolism in anther, especially the disorder of the alpha-linolenic acid metabolism pathway, leads to cotton male sterility[J]. >Journal of Integrative Agriculture, 2026, 25(2): 610-623.
[11] Teng Li, Shumei Wang, Qing Liu, Xuepeng Zhang, Lin Chen, Yuanquan Chen, Wangsheng Gao, Peng Sui. Effects of changing assimilate supply on starch synthesis in maize kernels under high temperature stress[J]. >Journal of Integrative Agriculture, 2026, 25(2): 639-647.
[12] Xiqiang Li, Yuhong Gao, Zhengjun Cui, Tingfeng Zhang, Shiyuan Chen, Shilei Xiang, Lingling Jia, Bin Yan, Yifan Wang, Lizhuo Guo, Bing Wu . Optimized nitrogen and potassium fertilizers application increases stem lodging resistance and grain yield of oil flax by enhancing lignin biosynthesis[J]. >Journal of Integrative Agriculture, 2026, 25(2): 659-670.
[13] Xin Wan, Dangjun Wang, Junya Li, Shuaiwen Zhang, Linyang Li, Minghui He, Zhiguo Li, Hao Jiang, Peng Chen, Yi Liu. Land use type shapes carbon pathways in Tibetan alpine ecosystems: Characterization of 13C abundance in aggregates and density fractions[J]. >Journal of Integrative Agriculture, 2026, 25(2): 448-459.
[14] Liyan Wang, Buqing Wang, Zhengmiao Deng, Yonghong Xie, Tao Wang, Feng Li, Shao’an Wu, Cong Hu, Xu Li, Zhiyong Hou, Jing Zeng Ye’ai Zou, Zelin Liu, Changhui Peng, Andrew Macrae. Surface soil organic carbon losses in Dongting Lake floodplain as evidenced by field observations from 2013 to 2022[J]. >Journal of Integrative Agriculture, 2026, 25(2): 436-447.
[15] Xi Chen, Khalid Ayesha, Xue Wen, Yanan Zhang, Mengru Dou, Kexuan Jia, Yong Wang, Yuling Li, Feng Sun, Guotian Liu, Yan Xu. An integrate methods to improve the high efficiency of embryo rescue breeding in seedless grapes[J]. >Journal of Integrative Agriculture, 2026, 25(2): 721-733.
No Suggested Reading articles found!