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Journal of Integrative Agriculture  2024, Vol. 23 Issue (8): 2557-2570    DOI: 10.1016/j.jia.2023.09.006
Special Issue: 小麦遗传育种Wheat Genetics · Breeding · Germplasm Resources
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Selection and application of four QTLs for grain protein content in modern wheat cultivars

Zihui Liu*, Xiangjun Lai*, Yijin Chen, Peng Zhao, Xiaoming Wang, Wanquan Ji, Shengbao Xu#

State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling 712100, China
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摘要  

籽粒蛋白质含量(GPC)是影响小麦籽粒营养成分的关键因素。本研究对406份小麦材料在8种环境中的GPC进行了重采样的全基因组关联分析(GWAS),鉴定到4个与GPC相关且被多次报道过的QTLs。通过对87个地方小麦和259个现代栽培品种的分析表明,这4个与GPC相关QTLs的优势单倍型在中国现代栽培品种中正逐渐缺失。但这些单倍型被中国不同小麦生产区采用后会对小麦产量及相关农艺性状产生重要影响。且其中大多数与GPC相关的QTLs对小麦产量没有显著的负面效应,表明可以利用它们在保持产量的同时提高小麦籽粒蛋白质含量。该研究可为今后利用这4个QTLs进行小麦籽粒蛋白质含量育种提供重要参考。



Abstract  
The grain protein content (GPC) is the key parameter for wheat grain nutritional quality.  This study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments, and identified four previously reported GPC QTLs.  An analysis of 87 landraces and 259 modern cultivars revealed the loss of superior GPC haplotypes, especially in Chinese cultivars.  These haplotypes were preferentially adopted in different agroecological zones and had broad effects on wheat yield and agronomic traits.  Most GPC QTLs did not significantly reduce yield, suggesting that high GPC can be achieved without a yield penalty.  The results of this study provide a reference for future GPC breeding in wheat using the four identified QTLs. 
Keywords:  breeding        grain protein content        haplotype selection and application        wheat  
Received: 29 March 2023   Accepted: 19 July 2023
Fund: 

This research was supported by the “Integration of Two Chains” Key Research and Development Projects of Shaanxi Province “Wheat Seed Industry Innovation Project”, China and the Key R&D of Yangling Seed Industry Innovation Center, China (Ylzy-xm-01).

About author:  Zihui Liu, E-mail: lzh@nwafu.edu.cn; Xiangjun Lai, E-mail: lxj2820@nwafu.edu.cn; #Correspondence Shengbao Xu, Mobile: +86-13303326363, E-mail: xushb@nwsuaf.edu.cn * These authors contributed equally to this study.

Cite this article: 

Zihui Liu, Xiangjun Lai, Yijin Chen, Peng Zhao, Xiaoming Wang, Wanquan Ji, Shengbao Xu. 2024. Selection and application of four QTLs for grain protein content in modern wheat cultivars. Journal of Integrative Agriculture, 23(8): 2557-2570.

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