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Journal of Integrative Agriculture  2017, Vol. 16 Issue (11): 2375-2385    DOI: 10.1016/S2095-3119(17)61683-6
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Identification of quantitative trait loci and candidate genes associated with ABA sensitivity in common wild rice (Oryza rufipogon Griff.)
CAI Wen-yang, TAN Lu-bin, LIU Feng-xia, SUN Chuan-qing
National Center for Evaluation of Agricultural Wild Plants (Rice)/Beijing Key Laboratory of Crop Genetic Improvement/Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, P.R.China
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Abstract  Abscisic acid (ABA), as one of the foremost signaling molecules in plants, is an important hormone which plays versatile functions in regulating developmental process and adaptive stress process.  A set of introgression lines were previously generated via a backcrossing program using an elite indica cultivar rice Teqing (O. sativa L.) as recipient and an accession of Yuanjiang common wild rice (O. rufipogon Griff.) as donor.  In this study, the previously developed introgression lines were evaluated for ABA sensitivity.  Here we reported that a total of 14 quantitative trait loci (QTLs) associated with ABA sensitivity were identified.  An ABA sensitive introgression line, YIL53, was identified and characterized.  Physiological characterization, including chlorophyll content, malondialdehyde content, soluble sugar content, and stomata movement, demonstrated that YIL53 exhibited the characteristics associated with ABA sensitivity.  Genotypic analysis revealed that YIL53 harbored one QTL related to ABA sensitivity, qASS1-2, which was located on chromosome 1 within one introgressed segment derived from the Yuanjiang common wild rice.  Furthermore, the qASS1-2 was finally narrowed down to a 441-kb region between simple sequence repeats (SSR) marker RM212 and single nucleotide polymorphism (SNP) marker M3 using the segregation population derived from the cross between Teqing and YIL53, and three candidate genes associated with ABA sensitivity were identified using a strategy combined gene expression analysis with QTL mapping.  Identification of the QTLs related to ABA sensitivity and characterization of the ABA sensitive line YIL53 would provide a helpful basis for isolating novel genes related to ABA sensitivity.  
Keywords:  wild rice       introgression lines       ABA sensitivity       quantitative trait locus       candidate gene  
Received: 09 December 2016   Accepted:

This work was supported by the Project of Conservation and Utilization of Agro-wild Plants of the Ministry of Agriculture of China and the Special Fund for Agro-scientific Research in the Public Interest, China (201003021).

Corresponding Authors:  Correspondence SUN Chuan-qing, Tel/Fax: +86-10-62731811, E-mail:   

Cite this article: 

CAI Wen-yang, TAN Lu-bin, LIU Feng-xia, SUN Chuan-qing. 2017. Identification of quantitative trait loci and candidate genes associated with ABA sensitivity in common wild rice (Oryza rufipogon Griff.). Journal of Integrative Agriculture, 16(11): 2375-2385.

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