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Journal of Integrative Agriculture  2023, Vol. 22 Issue (9): 2603-2616    DOI: 10.1016/j.jia.2023.02.013
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Dissecting the key genomic regions underlying high yield potential in common wheat variety ‘Kenong 9204’

ZHAO Chun-hua1*, ZHANG Na2*, FAN Xiao-li3, JI Jun4, 5, SHI Xiao-li4, CUI Fa1#, LING Hong-qing4#, LI Jun-ming5, 6#

1 College of Agriculture, Ludong University/Key Laboratory of Molecular Module-based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong, Yantai 264025, P.R.China
2 Jiangsu Xuhuai Regional Institute of Agricultural Sciences, Xuzhou 221131, P.R.China
3 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R.China
4 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijng 100101, P.R.China
5 Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, P.R.China
6 Key Laboratory of Molecular and Cellular Biology, Ministry of Education/Hebei Collaboration Innovation Center for Cell Signaling/Hebei Key Laboratory of Molecular and Cellular Biology/College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, P.R.China
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Abstract  The foundation parents play key roles in the genetic improvement of both yield potential and end-use quality in wheat.  Characterizing the genetic basis that underlies certain beneficial traits in the foundation parents will provide theoretical reference for molecular breeding by a design approach.  ‘Kenong 9204’ (KN9204) is a candidate foundation parent characterized by ideotype, high yield potential, and particularly high nitrogen fertilizer utilization.  To better understand the genetic basis of its high yield potential, high throughput whole-genome re-sequencing (10×) was performed on KN9204, its parental lines and its derivatives.  A high-resolution genetic composition map of KN9204 was constructed, which showed the parental origin of the favorable genomic segments based on the identification of excellent yield-related quantitative trait loci (QTL) from a bi-parental mapping population.  Xiaoyan 693 (XY693), a wheat–Thinopyrum ponticum partial amphidiploid, contributed a great deal to the high yield potential of KN9204, and three major stable QTLs from XY693 were fine mapped.  The transmissibility of key genomic segments from KN9204 to its derivatives were delineated, indicating that haplotype blocks containing beneficial gene combinations were conserved along with directional selection by breeders.  Evidence for selection sweeps in the breeding programs was identified.  This study provides a theoretical reference for the breeding of high-yield wheat varieties by a molecular design approach.
Keywords:  Kenong 9204        high-yielding potential        quantitative trait locus        genetic composition map        key genomic regions  
Received: 02 September 2022   Accepted: 14 November 2022
Fund: This research was jointly supported by the grants from the Shandong Major Basic Research Project of Natural Science Foundation, China (ZR2019ZD16), the Shandong Provincial Key Research and Development Program, China (2019GNC106126 and 2021LZGC009), the Natural Science Foundation of Hebei Province, China (C2021205013), the Hebei Scientific and Technological Innovation Team of Modern Wheat Seed Industry, China (21326318D), the National Natural Science Foundation of China (31871612, 31901535, and 32101726), and the China Agriculture Research System of MOF and MARA (CARS-03).

About author:  ZHAO Chun-hua, E-mail:; ZHANG Na,; #Correspondence CUI Fa, Tel: +86-535-6223193, E-mail:; LING Hong-qing, E-mail:; LI Jun-ming, E-mail: * These authors contributed equally to this study.

Cite this article: 

ZHAO Chun-hua, ZHANG Na, FAN Xiao-li, JI Jun, SHI Xiao-li, CUI Fa, LING Hong-qing, LI Jun-ming. 2023. Dissecting the key genomic regions underlying high yield potential in common wheat variety ‘Kenong 9204’. Journal of Integrative Agriculture, 22(9): 2603-2616.

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