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| Effect of feeding transgenic cry1Ab/cry1Ac rice on indices of immune function in broilers |
| LIU Ran-ran1, 2, ZHAO Gui-ping1, 2, ZHENG Mai-qing1, 2, LIU Jie1, ZHANG Jing-jing1, LI Peng1, LI Qing-he1, FENG Jing-hai1, 2, ZHANG Min-hong1, 2, WEN Jie1, 2 |
1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 State Key Laboratory of Animal Nutrition, Beijing 100193, P.R.China |
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Abstract The safety of feeding transgenic cry1Ab/cry1Ac rice (a genetically modified (GM) rice variety) to broilers was examined from an immunological perspective. Hatchling Arbor Acres chickens (240) were assigned to two dietary treatments (diets containing GM or non-GM rice) with 12 replicates per group and 10 birds per replicate. Traits were measured on one randomly selected bird from each replicate at d 21 and 42. The 42-d feeding trial revealed that cry1Ab/cry1Ac rice had no significant effect relative to non-GM rice on body weight and the immune organ indices. No significant pathological lesion in the spleen and bursa of Fabricius was found in the GM rice group. There were no significant differences in serum concentrations of immunoglobulin Y (IgY), IgM, interleukin 4 (IL-4) and IL-6 between the two groups at d 21 or 42, except for IL-6 being higher (P<0.05) in the GM-fed chickens at d 42. There were no differences in the T and B lymphocyte transformation rate and CD4+/CD8+ ratio between the two groups at d 42. Additionally, there was no significant difference between the two diets in expression of relevant genes viz. the major histocompatibility complex class II beta chain (BLB2), interferon beta 1 (IFNβ), tumour necrosis factor alpha-like (TNFα) and toll-like receptor 4 (TLR4) in the spleen and bursa of Fabricius. All the data demonstrated that transgenic cry1Ab/cry1Ac rice had no adverse effect on these aspects of immune function of broilers during 42-d feeding trial. Transgenic rice was therefore indistinguishable from non-GM rice in terms of short-term feeding in chickens.
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Received: 09 March 2015
Accepted:
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| Fund: The research was supported by the grants from the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX08011001-005), the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS04) and the Earmarked Fund for Modern Agro-Industry Technology Research System, China (CARS-42). |
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Corresponding Authors:
WEN Jie, Tel: +86-10-62815856, Fax: +86-10-62816018, E-mail: wenjie@caas.cn
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Cite this article:
LIU Ran-ran, ZHAO Gui-ping, ZHENG Mai-qing, LIU Jie, ZHANG Jing-jing, LI Peng, LI Qing-he, FENG Jing-hai, ZHANG Min-hong, WEN Jie.
2016.
Effect of feeding transgenic cry1Ab/cry1Ac rice on indices of immune function in broilers. Journal of Integrative Agriculture, 15(06): 1355-1363.
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