|
|
|
Genomic characterization and antimicrobial susceptibility of bovine intrauterine Escherichia coli and its relationship with postpartum uterine infections |
YANG Li-ming, WANG Yi-hao, PENG Yu, MIN Jiang-tao, HANG Su-qin, ZHU Wei-yun |
Laboratory of Gut Microbiology, Nanjing Agricultural University, Nanjing 210095, P.R.China |
|
|
Abstract To investigate the roles of Escherichia coli in the pathogenesis of postpartum uterine diseases in dairy cows, a total of 145 E. coli isolates were recovered from 18 healthy cows (61 isolates) and 25 cows with clinical endometritis (84 isolates) at 25–35 days after parturition. Genomic characteristics including phylogenetic grouping, genetic diversity and virulence genes of E. coli isolates were screened to profile the characteristics related to uterine infections. The susceptibility of the bacteria against 23 antibiotics was also evaluated to support prevention and treatment of clinical cases. Genetic diversity of E. coli identified by random amplification of polymorphic DNA (RAPD) revealed 103 clonal types, including 3 common types to unaffected cows and endometritis cows, 39 types specific to healthy cows and 61 types in endometritis subjects. In addition, the isolates from endometritis uteri showed more genetic variability compared with that of healthy cows. According to the findings of phylogenetic grouping, the E. coli isolates were assigned to group A (35.9%), B1 (59.3%) and D (4.8%). The expression of 10 of 20 virulence gens were detected positively, and only fimH gene was revealed significantly (P<0.05) associated with endometritis. From antimicrobial susceptibility test, E. coli was found highly resistant to tetracycline, ampicillin, carbenicillin and amoxicillin, but sensitive to amikacin, netilmicin, tobramycin, cefepime and ceftazidime. In conclusion, E. coli were extensively observed in both healthy and endometritis cows, and presented a large clonal types, however, fimH was the only gene observed associated with clinical endometritis. Our results suggest that the drugs like amikacin, netilmicin, tobramycin and cefepime could be considered for preventing and treating clinical endometritis in the practical management of dairy cow.
|
Received: 20 May 2015
Accepted:
|
Fund: The study was supported by the National Key Technology R&D Program of China (2011BAD19B02). |
Corresponding Authors:
HANG Su-qin, Tel: +86-25-84395037, Fax: +86-25-84395314, E-mail: suqinhang69@njau.edu.cn
|
About author: YANG Li-ming, Tel: +86-25-84395037, E-mail: 2014105026@njau.edu.cn |
Cite this article:
YANG Li-ming, WANG Yi-hao, PENG Yu, MIN Jiang-tao, HANG Su-qin, ZHU Wei-yun.
2016.
Genomic characterization and antimicrobial susceptibility of bovine intrauterine Escherichia coli and its relationship with postpartum uterine infections. Journal of Integrative Agriculture, 15(06): 1345-1354.
|
Abe C M, Salvador F A, Falsetti I N, Vieira M A, Blanco J, Blanco J E, Blanco M, Machado A M O, Elias W P, Hernandes R T, Gomes T A T. 2008. Uropathogenic Escherichia coli (UPEC) strains may carry virulence properties of diarrhoeagenic E. coli. FEMS Immunology & Medical Microbiology, 52, 397–406.Aronson M, Medalia O, Schori L, Mirelman D, Sharon N, Ofek I. 1979. Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl α-D-mannopyranoside. Journal of Infectious Diseases, 139, 329–332.Baumgart M, Dogan B, Rishniw M, Weitzman G, Bosworth B, Yantiss R, Orsi R H, Wiedmann M, McDonough P, Kim S G, Berg D, Schukken Y, Scherl E, Simpson K W. 2007. Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of Clostridiales in Crohn’s disease involving the ileum. The ISME Journal, 1, 403–418.Berg D E, Akopyants N S, Kersulyte D. 1994. Fingerprinting microbial genomes using the RAPD or AP-PCR method. Methods in Molecular and Cellular Biology, 5, 13–24.Bergthorsson U, Ochman H. 1998. Distribution of chromosome length variation in natural isolates of Escherichia coli. Molecular Biology and Evolution, 15, 6–16.Bicalho R, Machado V, Bicalho M, Gilbert R, Teixeira A, Caixeta L, Pereira R. 2010. Molecular and epidemiological characterization of bovine intrauterine Escherichia coli. Journal of Dairy Science, 93, 5818–5830.Brodzki P, Bochniarz M, Brodzki A, Wrona Z, Wawron W. 2014. Trueperella pyogenes and Escherichia coli as an etiological factor of endometritis in cows and the susceptibility of these bacteria to selected antibiotics. Polish Journal of Veterinary Sciences, 17, 657–664.Clermont O, Bonacorsi S, Bingen E. 2000. Rapid and simple determination of the Escherichia coli phylogenetic group. Applied and Environmental Microbiology, 66, 4555–4558.Drillich M, Voigt D, Forderung D, Heuwieser W. 2007. Treatment of acute puerperal metritis with flunixin meglumine in addition to antibiotic treatment. Journal of Dairy Science, 90, 3758–3763.Gündo?du A, Long Y, Katouli M. 2012. Prevalence and pathogenesis of extended-spectrum beta-lactamase producing Escherichia coli causing urinary tract infection in hospitalized patients. European Journal of Clinical Microbiology & Infectious Diseases, 31, 3107–3116.Gilbert R O, Shin S T, Guard C L, Erb H N, Frajblat M. 2005. Prevalence of endometritis and its effects on reproductive performance of dairy cows. Theriogenology, 64, 1879–1888.Hou Z Z, Xu W W. 2008. Isolation and identification of pathogenic bacterium of endometritis of dairy cows. Journal of Northeast Agricultural University, 39, 76–81. (in Chinese)Huang S H, Wan Z S, Chen Y H, Jong A Y, Kim K S. 2001. Further characterization of Escherichia coli brain microvascular endothelial cell invasion gene ibeA by deletion, complementation, and protein expression. Journal of Infectious Diseases, 183, 1071–1078.Johnson J R, Stell A L. 2000. Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host comprise. Journal of Infectious Disease, 181, 261–272.Kaper J B, Nataro J P, Mobley H L. 2004. Pathogenic Escherichia coli. National Review Microbiology, 2, 123–140.Kong X F, Liu Z G, Wang X T, Hu Y L. 2005. Isolation, identification and susceptibility testing of pathogenic bacterium of endometritis of a dairy farm in Nanjing. Chinese Journal of Veterinary Medicine, 41, 24–25. (in Chinese)Krekeler N, Marenda M, Browning G, Holden K, Charles J, Wright P. 2012. Uropathogenic virulence factor FimH facilitates binding of uteropathogenic Escherichia coli to canine endometrium. Comparative Immunology, Microbiology and Infectious Diseases, 35, 461–467.Langermann S, Palaszynski S, Barnhart M, Auguste G, Pinkner J S, Burlein J, Barren P, Koenig S, Leath S, Jones C H, Hultgren S J. 1997. Prevention of mucosal Escherichia coli infection by FimH-adhesin-based systemic vaccination. Science, 276, 607–611.LeBlanc S, Duffield T, Leslie K, Bateman K, Keefe G P, Walton J, Johnson W. 2002. Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. Journal of Dairy Science, 85, 2223–2236.Madico G, Akopyants N S, Berg D E. 1995. Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157:H7 strains by using templates from boiled cultures. Journal of Clinical Microbiology, 33, 1534–1536.Malinowski E, Lassa H, Markiewicz H, Kaptur M, Nadolny M, Niewitecki W, Zi?tara J. 2011. Sensitivity to antibiotics of Arcanobacterium pyogenes and Escherichia coli from the uteri of cows with metritis/endometritis. The Veterinary Journal, 187, 234–238.NRC (National Research Council). 2001. Nutrient Requirements of Dairy Cattle. 7th ed. National Academy Press, Washington, D.C. Ofek I, Mirelman D, Sharon N. 1977. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature, 265, 623–625.Peng Y, Wang Y H, Hang S Q, Zhu W Y. 2013. Microbial diversity in uterus of healthy and metritic postpartum Holstein dairy cows. Folia Microbiol (Praha), 58, 593–600.Picard B, Garcia J S, Gouriou S, Duriez P, Brahimi N, Bingen E, Elion J, Denamur E. 1999. The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infection and Immunity, 67, 546–553.Qin J C, Cao G B, Shi K W, Deng L X, Shi K L, Li Z G, Qin R F, Wang X Z. 2012. Isolation and identification of bacteria from uterus of Jiaxian Red Cattle. Acta Agriculturae Boreali-occidentalis Sinica, 21, 21–25. (in Chinese).Sheldon I M, Rycroft A N, Dogan B, Craven M, Bromfield J J, Chandler A, Roberts M H, Price S B, Gilbert R O, Simpson K W. 2010. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. PLoS ONE, 5, e9192.Sheldon I, Noakes D, Rycroft A, Pfeiffer D, Dobson H. 2002. Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Reproduction, 123, 837–845.Silva E, Gaivao M, Leitao S, Jost B, Carneiro C, Vilela C, Lopes da Costa L, Mateus L. 2008. Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis. Veterinary Microbiology, 132, 111–118.Silva E, Leitão S, Tenreiro T, Pomba C, Nunes T, da Costa LL, Mateus L. 2009. Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows. Journal of Dairy Science, 92, 6000–6010.Torres A G, Zhou X, Kaper J B. 2005. Adherence of diarrheagenic Escherichia coli strains to epithelial cells. Infection and Immunity, 73, 18–29.Watts J L, Shryock T R, Apley M, Bade D J, Brown S D, Gray J T, Heine H, Hunter R P, Mevius D J, Papich M G, Silley P, Zurenko G E. 2008. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals: Approved Standard. 3rd ed. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania. Document M31–A3, 28(8).Williams E J, Fischer D P, Noakes D E, England G C, Rycroft A, Dobson H, Sheldon I M. 2007. The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow. Theriogenology, 68, 549–559.Williams E J, Fischer D P, Pfeiffer D U, England G C, Noakes D E, Dobson H, Sheldon I M. 2005. Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology, 63, 102–117.Wang G Q, Tian W R, Ye X H. 2001. The uterine bacteria in normal postpartum cows. Heilongjiang Journal of Animal Science and Veterinary Medicine, (3), 3–5. (in Chinese)Wang Y, Chai T, Miao Z. 2010. ERIC-PCR identification of the spread of airborne Escherichia coli in pig houses. Science of the Total Environment, 408, 1446–1450. |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|