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Characterization of Ppd-D1 alleles on the developmental traits and rhythmic expression of photoperiod genes in common wheat |
ZHAO Yong-ying, WANG Xiang, WEI Li, WANG Jing-xuan, YIN Jun |
1、National Engineering Research Center for Wheat/Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of
Wheat and Maize Crop Science/College of Agriculture, Henan Agricultural University, Zhengzhou 450002, P.R.China
2、National Laboratory of Wheat Engineering/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-Huai Region,
Ministry of Agricultural/Key Laboratory of Wheat Biology of Henan Province/Wheat Research Institution, Henan Academy of
Agricultural Sciences, Zhengzhou 450002, P.R.China |
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摘要 Photoperiodic response is an important characteristic that plays an important role in plant adaptability for various environments. Wheat cultivars grow widely and have high yield potential for the strong photoperiod adaptibility. To assess the photoperiodic response of different genotypes in wheat cultivars, the photoperiodic effects of the Ppd-D1 alleles and the expressions of the related TaGI, TaCO and TaFT genes in Liaochun 10 and Ningchun 36 were investigated under the short-day (6 h light, SD), moderate-day (12 h light, MD) and long-day (24 h light, LD) conditions. Amplicon length comparison indicated that the promoter of Ppd-D1 in Ningchun 36 is intact, while Liaochun 10 presented the partial sequence deletion of Ppd-D1 promoter. The durations of all developmental stages of the two cultivars were reduced by subjection to an extended photoperiod, except for the stamen and pistil differentiation stage in the Liaochun 10 cultivar. The expression levels of the Ppd-D1 alleles and the TaGI, TaCO and TaFT genes associated with the photoperiod pathway were examined over a 24-h period under SD and MD conditions. The relationships of different photoperiodic responses of the two cultivars and the expression of photoperiod pathway genes were analyzed accordingly. The photoperiod insensitive (PI) genotype plants flower early under SD; meanwhile, the abnormal expression of the Ppd-D1a allele is accompanied with an increase in TaFT1 expression and the TaCO expression variation. The results would facilitate molecular breeding in wheat.
Abstract Photoperiodic response is an important characteristic that plays an important role in plant adaptability for various environments. Wheat cultivars grow widely and have high yield potential for the strong photoperiod adaptibility. To assess the photoperiodic response of different genotypes in wheat cultivars, the photoperiodic effects of the Ppd-D1 alleles and the expressions of the related TaGI, TaCO and TaFT genes in Liaochun 10 and Ningchun 36 were investigated under the short-day (6 h light, SD), moderate-day (12 h light, MD) and long-day (24 h light, LD) conditions. Amplicon length comparison indicated that the promoter of Ppd-D1 in Ningchun 36 is intact, while Liaochun 10 presented the partial sequence deletion of Ppd-D1 promoter. The durations of all developmental stages of the two cultivars were reduced by subjection to an extended photoperiod, except for the stamen and pistil differentiation stage in the Liaochun 10 cultivar. The expression levels of the Ppd-D1 alleles and the TaGI, TaCO and TaFT genes associated with the photoperiod pathway were examined over a 24-h period under SD and MD conditions. The relationships of different photoperiodic responses of the two cultivars and the expression of photoperiod pathway genes were analyzed accordingly. The photoperiod insensitive (PI) genotype plants flower early under SD; meanwhile, the abnormal expression of the Ppd-D1a allele is accompanied with an increase in TaFT1 expression and the TaCO expression variation. The results would facilitate molecular breeding in wheat.
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Received: 11 February 2015
Accepted:
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Fund: This research was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B07, 2013BAD04B01) and the National Natural Science Foundation of China (31271726). |
Corresponding Authors:
YIN Jun, Tel/Fax: +86-371-63558203, E-mail: xmzxyj@126.com
E-mail: xmzxyj@126.com
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About author: ZHAO Yong-ying, Mobile: +86-13513895016, Tel: +86-371-65750512, E-mail: yongying001@126.com;* These authors contributed equally to this study. |
Cite this article:
ZHAO Yong-ying, WANG Xiang, WEI Li, WANG Jing-xuan, YIN Jun.
2016.
Characterization of Ppd-D1 alleles on the developmental traits and rhythmic expression of photoperiod genes in common wheat. Journal of Integrative Agriculture, 15(3): 502-511.
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Beales J, Turner A, Griffiths S, Snape J W, Laurie D A.2007. A Pseudo-response regulator is misexpressed inthe photoperiod insensitive Ppd-D1a mutant of wheat(Triticum aestivum L.). Theoretical and Applied Genetics,115, 721-733Bentley A R, Turner A S, Gosman N, Leigh F J, Maccaferri M,Dreisigacker S, Greenland A, Laurie D A. 2011. Frequencyof photoperiod insensitive Ppd-A1a alleles in tetraploid,hexaploid and synthetic hexaploid wheat germplasm. PlantBreeding, 130, 10-15Dubcovsky J, Loukoianon A, Fu D L, Valarik M, Sanchez A, YanL L. 2006. Effect of photoperiod on the regulation of wheatvernalization genes VRN1 and VRN2. Plant MolecularBiology, 60, 469-480Dyck J A, Matus-Cadiz M A, Hucl P,Talbert L, Hunt T, Dubuc JP, Nass H, Clayton G, Dobb J, Quick J. 2004. Agronomicperformance of hard red spring wheat isolines sensitive andinsensitive to photoperiod. Crop Science, 44, 1976-1981Foulkes M J, Sylvester-Bradley R, Worland A J, Snape J W.2004. Effects of a photoperiod-response gene Ppd-D1 onyield potential and drought resistance in UK winter wheat.Euphytica, 135, 63-73Gonzalez F G, Slafer G A, Miralles D J. 2002. Vernalization andphotoperiod responses in wheat pre-flowering reproductivephases. Field Crops Research, 74, 183-195Gonzalez F G, Slafer G A, Miralles D J. 2005. Pre-anthesisdevelopment and number of fertile florets in wheat asaffected by photoperiod sensitivity genes Ppd-D1 andPpd-B1. Euphytica, 146, 253-269Guo Z A, Song Y X, Zhou R H, Ren Z L, Jia J Z. 2010. Discovery,evaluation and distribution of haplotypes of the wheatPpd-D1 gene. New Phytologist, 185, 841-851Jokela V, Virkajärvi P, Tanskanen J, Seppänen M M. 2014.Vernalization, gibberellic acid and photo period areimportant signals of yield formation in timothy (Phleumpratense). Physiologia Plantarum, doi: 10.1111/ppl.12141Kitagawa S, Shimada S, Murai K. 2012. Effect of Ppd-1 onthe expression of flowering-time genes in vegetative andreproductive growth stages of wheat. Genes & GeneticSystems, 87, 161-168Kumar S, Sharma V, Chaudhary S, Tyagi A, Mishra P,Priyadarshini A, Singh A. 2012. Genetics of flowering time inbread wheat Triticum aestivum: Complementary interactionbetween vernalization-insensitive and photoperiodinsensitivemutations imparts very early flowering habit tospring wheat. Journal of Genetic, 91, 33-47Livak K J, Schmittgen T D. 2001. Analysis of relative geneexpression data using real-time quantitative PCR and the2-ΔΔCT method. Methods, 25, 402–408.Law C N, Sutka J, Worland A J. 1978. A genetic study of daylengthresponse in wheat. Heredity, 41, 575–585.Lobell D B, Field C B. 2007. Global scale climate-cropyield relationships and the impacts of recent warming.Environmental Research Letters, 2, 1–7.Locke J C W, Kozma-Bognar L, Gould P D, Feher B, Kevei E,Nagy F, Turner M S, Hall A, Millar A J. 2006. Experimentalvalidation of a predicted feedback loop in the multi-oscillatorclock of Arabidopsis thaliana. Molecular Systems Biology,2, 59.Matsushika A, Makino S, Kojima M, Mizuno T. 2000. Circadianwaves of expression of the APRR1/TOC1 family of pseudoresponseregulators in Arabidopsis thaliana: Insight intothe plant circadian clock. Plant and Cell Physiology, 41,1002–1012.McIntosh R A, Yamazaki Y, Devos K M, Dubcovsky J, RogersW J, Appels R. 2003. Catalogue of gene symbols for wheat.[2011-03-10] http://wheat.pw.usda.gov/ggpages/wgc/2003/Murakami M, Ashikari M, Miura K, Yamashino T, Mizuno T.2003. The evolutionarily conserved OsPRR quintet: Ricepseudo-response regulators implicated in circadian rhythm.Plant and Cell Physiology, 44, 1229-1236Nishida H, Yoshida T, Kawakami K, Fujita M, Long B, AkashiY, Laurie D A, Kato K. 2013. Structural variation in the 5´upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat (Triticumaestivum L.) and their effect on heading time. MolecularBreeding, 31, 27-37Porebski S, BaileyL G, Baum B. 1997. Modification of aCTAB DNA extraction protocol for plants containinghigh polysaccharide and polyphenol components. PlantMolecular Biology Reporter, 15, 8-15Pokhilko A, Fernández A P, Edwards K D, Southern M M,Halliday K J, Millar A J. 2012. The clock gene circuitin Arabidopsis includes a repressilator with additionalfeedback loops. Molecular Systems Biology, 8, 574.Shaw L M, Turner A S, Laurie D A. 2012. The impactof photoperiod insensitive Ppd-1a mutations on thephotoperiod pathway across the three genomes ofhexaploid wheat (Triticum aestivum). The Plant Journal,71, 71-84Scarth R, Law C N. 1983. The location of the photoperiod gene,Ppd-B1 and an additional genetic factor for ear-emergencetime on chromosome 2B of wheat. Heredity, 51, 607-619Scarth R, Law C N. 1984. The control of the day-length responsein wheat by the group 2 chromosomes. Zeitschrift fürPflanzenzüchtung, 92, 140-150Seki M, Chono M, Matsunaka H, Fujita M, Oda S, Kubo K,Kiribuchi-Otobe C, Kojima H, Nishida H, Kato K. 2011.Distribution of photoperiod-insensitive alleles Ppd-B1a andPpd-D1a and their effect on heading time in Japanese wheatcultivars. Breeding Science, 61, 405-412Sibony M, Pinthus M J. 1988. Floret initiation and developmentin spring wheat (Triticum aestivum L.). Annals of Botany,61, 473-479Turner A, Beales J, Faure S, Dunford R P, Laurie D A. 2005.The pseudo response regulator Ppd-H1 provides adaptationto photoperiod in barley. Science, 310, 1031-1034Tanio M, Kato K. 2007. Development of near-isogenic lines forphotoperiod-insensitive genes, Ppd-B1 and Ppd-D1, carriedby Japanese wheat cultivars and their effect on apicaldevelopment. Breeding Science, 57, 65-72Wilhelm E P, Turner A S, Laurie D A. 2009. Photoperiodinsensitive Ppd-A1a mutations in tetraploid wheat (Triticumdurum Desf.). Theoretical and Applied Genetics, 118,285-294Worland A J. 1996. The influence of flowering time genes onenvironmental adaptability in European wheat. Euphytica,89, 49-57Welsh J R, Keim D L, Pirasteh B, Richards R D. 1973. Geneticcontrol of photoperiod response in wheat. In: Sears E R,Sears L M S, eds., Proceedings of the 4th InternationalWheat Genetic Symposium. University of Missouri Press,Columbia, MO, USA. pp. 897-884Whitechurch E M, Slafer G A. 2002. Contrasting Ppd allelesin wheat: effects on sensitivity to photoperiod in differentphases. Field Crops Research, 73, 95-105Whitechurch E M, Slafer G A. 2001. Responses to photoperiodbefore and after jointing in wheat substitution lines.Euphytica, 118, 47-51Yang F P, Zhang X K, Xia X C, Laurie D A. Yang W X, He Z H.2009. Distribution of the photoperiod insensitive Ppd-D1aallele in Chinese wheat cultivars. Euphytica, 165, 445-452 |
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