Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9
HAN Liu-sha, LI Zai-feng, WANG Jia-zhen, SHI Ling-zhi, ZHU Lin, LI Xing, LIU Da-qun, Syed J A Shah
1、College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
2、Nuclear Institute for Food and Agricuture, Tarnab, Peshawar 25000, Pakistan
摘要 The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. In order to decipher the genetic base of resistance in Yu 356-9, gene postulation, inheritance analyses, and chromosome linkage mapping were carried out. Gene postulation completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 (resistant)/Zhengzhou 5389 (susceptible) were tested with leaf rust pathotype “FHNQ” in the greenhouse. Results indicated a 3:1 segregation ratio, indicative of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was temporarily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat (SSR) markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.
Abstract The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. In order to decipher the genetic base of resistance in Yu 356-9, gene postulation, inheritance analyses, and chromosome linkage mapping were carried out. Gene postulation completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 (resistant)/Zhengzhou 5389 (susceptible) were tested with leaf rust pathotype “FHNQ” in the greenhouse. Results indicated a 3:1 segregation ratio, indicative of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was temporarily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat (SSR) markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.
This study was supported by the National Natural Science Foundation of China (International/Regional Cooperation and Exchange Program) (31361140367) and the Hebei Provincial Outstanding Youth Project, China (YQ2013024).
Corresponding Authors:
LI Xing, Mobile: +86-13513220265, Fax: +86-312-7528500, E-mail: lixing@hebau.edu.cn; LIU Da-qun,Tel/Fax: +86-312-7528500, E-mail: ldq@hebau.edu.cn
E-mail: lixing@hebau.edu.cn;ldq@hebau.edu.cn
About author: HAN Liu-sha, Tel: +86-312-7528500, E-mail: LXKZH@163.com;* These authors contributed equally to this study.
HAN Liu-sha, LI Zai-feng, WANG Jia-zhen, SHI Ling-zhi, ZHU Lin, LI Xing, LIU Da-qun, Syed J A Shah.
2015.
Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9. Journal of Integrative Agriculture, 14(7): 1223-1228.
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