Please wait a minute...
Journal of Integrative Agriculture
Advanced Online Publication | Current Issue | Archive | Adv Search
CRISPR/Cas9-mediated mutagenesis of transcriptional repressor SlMYB32 improves flavonols and flavanones accumulation in tomato fruit

Ruining Zhang1, 2, Yunlin Cao1, 2, Tong Zhang1, 2, Yingyue Ma1, 2, Jiajia Li1, 2, Kunsong Chen1, 2, Xian Li1, 2#

1 College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China

2 Zhejiang Provincial Key Laboratory of Horticultural Crop Quality Manipulation, Zhejiang University, Hangzhou 310058, China 

 Highlights 

l SlMYB32 was identified as a repressor regulating phenylpropanoid pathway

l CRISPR/Cas9-mediated mutagenesis of SlMYB32 results in flavonol-rich tomato with 1 mg g-1 FW rutin

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

黄酮醇和黄烷酮是重要的生物活性物质,具有多样的药理学活性和保健功能。黄酮醇和黄烷酮生物合成的转录激活研究较为广泛,但相关负调节因子信息很少。CRISPR/Cas9 基因编辑技术可以对基因进行精确修饰,是培育生物强化材料和探索潜在分子机制的重要方法。本研究鉴定了番茄果实中的转录抑制子SlMYB32,基因编辑敲除SlMYB32显著提高了番茄果实黄酮醇与黄烷酮含量,其中槲皮素3-O-芸香糖苷(芦丁)高达1 mg g-1鲜重。转录组分析表明,slmyb32突变体中SlPAL6Sl4CL3Sl4CL4及五个候选SlUGTs的基因表达显著提高;双荧光素酶检测与EMSA分析发现SlMYB32可以与SlPAL6Sl4CL3的启动子结合并抑制其转录活性;此外,slmyb32突变体中27个转录因子发生了显著差异表达,包括与已知类黄酮调控因子聚类在相同分支的2SlMYBs2SlNACs2SlAP2s1SlWRKY,它们是今后研究SlMYB32调控网络的重要候选转录因子。本研究为果实生物活性物质的改良及黄酮醇和黄烷酮生物合成的负调控机制提供了新见解。



Abstract  

Flavonols and flavanones are important bioactive compounds with multiple pharmacological activities and health benefits. Transcriptional activation of flavonol and flavanone biosynthesis has been studied extensively, while little is known about the negative regulators. CRISPR/Cas9 gene-editing technology, with the advantage of precise genetic modification, is a desirable tool for breeding biofortified materials and exploring potential molecular mechanisms. In this study, a transcriptional repressor, SlMYB32, was characterized in tomato fruit. Phenotype and metabolome analysis confirmed that knockout of SlMYB32 resulted in increased accumulation of flavonols and flavanones, especially about 1 mg g-1 FW of quercetin 3-O-rutinoside (rutin). Transcriptome analysis indicated that expression of key genes SlPAL6, Sl4CL3 and Sl4CL4 as well as five candidate SlUGTs were significantly up-regulated in slmyb32 mutants. Dual-luciferase and EMSA assay indicated SlMYB32 could bind to and repress promoter activities of SlPAL6 and Sl4CL3. Expression of 27 transcription factors belonging to twelve families was significantly changed in slmyb32 mutants, among which two SlMYBs, two SlNACs, two SlAP2s and one SlWRKY were clustered with known flavonoid regulators. Our results provide new insights into improving bioactive compounds in fruit and understanding negative regulatory mechanisms in flavonol and flavanone biosynthesis.

 

Keywords:  Flavonol              Flavanone              CRISPR/Cas9              Repressor SlMYB32              Tomato              Transcriptional regulation  
Online: 13 November 2025  
Fund: 

The research was supported by the National Natural Science Foundation of China (32372667). 

About author:  Ruining Zhang, E-mail: rnzh@zju.edu.cn; #Correspondence Xian Li, Tel: +86-13957187980, Email: xianli@zju.edu.cn

Cite this article: 

Ruining Zhang, Yunlin Cao, Tong Zhang, Yingyue Ma, Jiajia Li, Kunsong Chen, Xian Li. 2025. CRISPR/Cas9-mediated mutagenesis of transcriptional repressor SlMYB32 improves flavonols and flavanones accumulation in tomato fruit. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.11.011

Albert N W, Davies K M, Lewis D H, Zhang H, Montefiori M, Brendolise C, Boase M R, Ngo H, Jameson P E, Schwinn K E. 2014. A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots. The Plant Cell, 26, 962–980.

Ali M Y, Zamponi G W, Abdul Q A, Seong S H, Min B-S, Jung H A, Choi J S. 2024. Prunin from Poncirus trifoliata (L.) Rafin inhibits aldose reductase and glucose-fructose–mediated protein glycation and oxidation of human serum albumin. Journal of Agricultural and Food Chemistry, 72, 7203–7218.

Bhakta S, Negi S, Tak H, Singh S, Ganapathi T R. 2022. MusaATAF2-like protein regulates shoot development and multiplication by inducing cytokinin hypersensitivity and flavonoid accumulation in banana plants. Plant Cell Reports, 41, 1197–1208.

Cao Y, Zhang R, Xing M, Ren C, Li J, Qian J, Mei Y, Yang X, Sun C, Grierson D, Chen K, Xu C, Li X. 2023. Synergistic actions of three MYB transcription factors underpins the high accumulation of myricetin in Morella rubra. The Plant Journal, 115, 577-594.

Cao Y, Mei Y, Zhang R, Zhong Z, Yang X, Xu C, Chen K, Li X. 2024a. Transcriptional regulation of flavonol biosynthesis in plants. Horticulture Research, 11, uhae043.

Cao Y, Zhang R, Ou R, Mei Y, Cui X, Zhang T, Yang X, Shi Y, Zhao X, Xu C, Chen K, Li X. 2024b. Overexpression of MrMYB12 or MrMYB12/MrF3’5’H results in flavonol-rich tomato fruit that improves resistance against Botrytis cinerea during the shelf life period. Postharvest Biology and Technology, 211, 112818.

Cavallini E, Matus J T, Finezzo L, Zenoni S, Loyola R, Guzzo F, Schlechter R, Ageorges A, Arce-Johnson P, Tornielli G B. 2015. The phenylpropanoid pathway is controlled at different branches by a set of R2R3-MYB C2 repressors in grapevine. Plant Physiology, 167, 1448–1470.

Chen C, Wu Y, Li J, Wang X, Zeng Z, Xu J, Liu Y, Feng J, Chen H, He Y, Xia R. 2023. TBtools-II: A “one for all, all for one” bioinformatics platform for biological big-data mining. Molecular Plant, 16, 1733–1742.

Cong L, Qu Y, Sha G, Zhang S, Ma Y, Chen M, Zhai R, Yang C, Xu L, Wang Z. 2021. PbWRKY75 promotes anthocyanin synthesis by activating PbDFR, PbUFGT, and PbMYB10b in pear. Physiologia Plantarum, 173, 1841–1849.

Czemmel S, Stracke R, Weisshaar B, Cordon N, Harris N N, Walker A R, Robinson S P, Bogs J. 2009. The grapevine R2R3-MYB transcription factor VvMYBF1 regulates flavonol synthesis in developing grape berries. Plant Physiology, 151, 1513–1530.

Deng X, Cheng L, Qiao Y, Liu X, Zhou Y, Liu H, Wang L. 2024. Rutin ameliorates HCD-induced cholesterol metabolism disorder in zebrafish larvae revealed by transcriptome and metabolome analysis. Phytomedicine, 135, 156058.

Dong R, Zhang X, Liu Y, Zhao T, Sun Z, Liu P, Xiang Q, Xiong J, Du X, Yang X, Gui D, Xu Y. 2023. Rutin alleviates EndMT by restoring autophagy through inhibiting HDAC1 via PI3K/AKT/mTOR pathway in diabetic kidney disease. Phytomedicine, 112, 154700.

Funaki A, Waki T, Noguchi A, Kawai Y, Yamashita S, Takahashi S, Nakayama T. 2015. Identification of a highly specific isoflavone 7-O-glucosyltransferase in the soybean (Glycine max (L.) Merr.). Plant and Cell Physiology, 56, 1512-1520.

Gao Y, Lin Y, Xu M, Bian H, Zhang C, Wang J, Wang H, Xu Y, Niu Q, Zuo J, Fu D, Pan Y, Chen K, Klee H, Lang Z, Zhang B. 2022. The role and interaction between transcription factor NAC‐NOR and DNA demethylase SlDML2 in the biosynthesis of tomato fruit flavor volatiles. New Phytologist, 235, 1913–1926.

Griesser M, Vitzthum F, Fink B, Bellido M L, Raasch C, Munoz-Blanco J, Schwab W. 2008. Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria×ananassa) achene and receptacle. Journal of Experimental Botany, 59, 2611–2625.

Han X, Zhao S, Song H, Xu T, Fang Q, Hu G, Sun L. 2021. Kaempferol alleviates LD-mitochondrial damage by promoting autophagy: Implications in Parkinson’s disease. Redox Biology, 41, 101911.

Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C. 2000. Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. The EMBO Journal, 19, 6150–6161.

Jun J H, Liu C, Xiao X, Dixon R A. 2015. The transcriptional repressor MYB2 regulates both spatial and temporal patterns of proanthocyandin and anthocyanin pigmentation in Medicago truncatula. The Plant Cell, 27, 2860–2879.

Khan I A, Cao K, Guo J, Li Y, Wang Q, Yang X, Wu J, Fang W, Wang L. 2022. Identification of key gene networks controlling anthocyanin biosynthesis in peach flower. Plant Science, 316, 111151.

Kumar S P, Birundha K, Kaveri K, Devi K T R. 2015. Antioxidant studies of chitosan nanoparticles containing naringenin and their cytotoxicity effects in lung cancer cells. International Journal of Biological Macromolecules, 78, 87–95.

Li H, Li Y, Yu J, Wu T, Zhang J, Tian J, Yao Y. 2020a. MdMYB8 is associated with flavonol biosynthesis via the activation of the MdFLS promoter in the fruits of Malus crabapple. Horticulture Research, 7, 19.

Li J, Scarano A, Gonzalez N M, D’Orso F, Yue Y, Nemeth K, Saalbach G, Hill L, De Oliveira Martins C, Moran R, Santino A, Martin C. 2022a. Biofortified tomatoes provide a new route to vitamin D sufficiency. Nature Plants, 8, 611–616.

Li Y, Shan X, Gao R, Han T, Zhang J, Wang Y, Kimani S, Wang L, Gao X. 2020b. MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida. Communications Biology, 3, 396.

Li Z, Zhang Y, Ren J, Jia F, Zeng H, Li G, Yang X. 2022b. Ethylene‐responsive factor ERF114 mediates fungal pathogen effector PevD1‐induced disease resistance in Arabidopsis thaliana. Molecular Plant Pathology, 23, 819–831.

Lin S, Li H, Tao Y, Liu J, Yuan W, Chen Y, Liu Y, Liu S. 2020. In vitro and in vivo evaluation of membrane-active flavone amphiphiles: Semisynthetic kaempferol-derived antimicrobials against drug-resistant gram-positive bacteria. Journal of Medicinal Chemistry, 63, 5797–5815.

Liu G, Li C, Yu H, Tao P, Yuan L, Ye J, Chen W, Wang Y, Ge P, Zhang J, Zhou G, Zheng W, Ye Z, Zhang Y. 2020. GREEN STRIPE, encoding methylated TOMATO AGAMOUS‐LIKE 1, regulates chloroplast development and Chl synthesis in fruit. New Phytologist, 228, 302–317.

Liu J, Osbourn A, Ma P. 2015. MYB transcription factors as regulators of phenylpropanoid metabolism in plants. Molecular Plant, 8, 689–708.

Liu X, Lin C, Ma X, Tan Y, Wang J, Zeng M. 2018. Functional characterization of a flavonoid glycosyltransferase in sweet orange (Citrus sinensis). Frontiers in Plant Science, 9, 166.

Love M I, Huber W, Anders S. 2014. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15, 550.

Ma D, Constabel C P. 2019. MYB repressors as regulators of phenylpropanoid metabolism in plants. Trends in Plant Science, 24, 275–289.

Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu Y-G. 2015. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Molecular Plant, 8, 1274–1284.

Modolo LV, Li L, Pan H, Blount JW, Dixon RA, Wang X. 2009. Crystal structures of glycosyltransferase UGT78G1 reveal the molecular basis for glycosylation and deglycosylation of (iso)flavonoids. Journal of Molecular Biology, 392, 1292-1302.

Morishita T, Kojima Y, Maruta T, Nishizawa-Yokoi A, Yabuta Y, Shigeoka S. 2009. Arabidopsis NAC transcription factor, ANAC078, regulates flavonoid biosynthesis under high-light. Plant and Cell Physiology, 50, 2210–2222.

Murata K, Kitano T, Yoshimoto R, Takata R, Ube N, Ueno K, Ueno M, Yabuta Y, Teraishi M, Holland C K, Jander G, Okumoto Y, Mori N, Ishihara A. 2020. Natural variation in the expression and catalytic activity of a naringenin 7‐O‐methyltransferase influences antifungal defenses in diverse rice cultivars. The Plant Journal, 101, 1103–1117.

Noguchi A, Horikawa M, Fukui Y, Fukuchi-Mizutani M, Iuchi-Okada A, Ishiguro M, Kiso Y, Nakayama T, Ono E. 2009. Local differentiation of sugar donor specificity of flavonoid glycosyltransferase in Lamiales. The Plant Cell, 21, 1556-1572.

Nonaka S, Arai C, Takayama M, Matsukura C, Ezura H. 2017. Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis. Scientific Reports, 7, 7057.

Pathak S, Regmi S, Nguyen T T, Gupta B, Gautam M, Yong C S, Kim J O, Son Y, Kim J-R, Park M H, Bae Y K, Park S Y, Jeong D, Yook S, Jeong J-H. 2018. Polymeric microsphere-facilitated site-specific delivery of quercetin prevents senescence of pancreatic islets in vivo and improves transplantation outcomes in mouse model of diabetes. Acta Biomaterialia, 75, 287–299.

Ren C, Guo Y, Xie L, Zhao Z, Xing M, Cao Y, Liu Y, Lin J, Grierson D, Zhang B, Xu C, Chen K, Li X. 2022. Identification of UDP-rhamnosyltransferases and UDP-galactosyltransferase involved in flavonol glycosylation in Morella rubra. Horticulture Research, 9, uhac138.

Rodríguez-Leal D, Lemmon Z H, Man J, Bartlett M E, Lippman Z B. 2017. Engineering quantitative trait variation for crop improvement by genome editing. Cell, 171, 470-480.e8.

Ruan Y, Zhu X, Shen J, Chen H, Zhou G. 2024. Mechanism of Nicotiflorin in San-Ye-Qing rhizome for anti-inflammatory effect in ulcerative colitis. Phytomedicine, 129, 155564.

Saxena S, Pal L, Naik J, Singh Y, Verma P K, Chattopadhyay D, Pandey A. 2023. The R2R3‐MYB‐SG7 transcription factor CaMYB39 orchestrates surface phenylpropanoid metabolism and pathogen resistance in chickpea. New Phytologist, 238, 798–816.

Shen H, He X, Poovaiah C R, Wuddineh W A, Ma J, Mann D G J, Wang H, Jackson L, Tang Y, Neal Stewart C, Chen F, Dixon R A. 2012. Functional characterization of the switchgrass (Panicum virgatum) R2R3‐MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks. New Phytologist, 193, 121–136.

Shi Y, Dai Z, Chen K. 2024. Transcription factors involved in tomato fruit quality improvement. Food Quality and Safety, 8, fyad050.

Tang Y, Zhang L, Sun R, Luo B, Zhou Y, Zhang Y, Liang Y, Xiao B, Wang C. 2023. Pulmonary delivery of mucus-traversing PF127-modified silk fibroin nanoparticles loading with quercetin for lung cancer therapy. Asian Journal of Pharmaceutical Sciences, 18, 100833.

Tohge T, Nishiyama Y, Hirai M Y, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe D B, Kitayama M, Noji M, Yamazaki M, Saito K. 2005. Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor. The Plant Journal, 42, 218-235.

Tohge T, Fernie A R. 2016. Specialized metabolites of the flavonol class mediate root phototropism and growth. Molecular Plant, 9, 1554–1555.

Ulusoy H G, Sanlier N. 2020. A minireview of quercetin: From its metabolism to possible mechanisms of its biological activities. Critical Reviews in Food Science and Nutrition, 60, 3290–3303.

Wang S, Zhang Z, Li L, Wang H, Zhou H, Chen X, Feng S. 2022. Apple MdMYB306‐like inhibits anthocyanin synthesis by directly interacting with MdMYB17 and MdbHLH33. The Plant Journal, 110, 1021–1034.

Xing M, Cao Y, Grierson D, Sun C, Li X. 2021a. The chemistry, distribution, and metabolic modifications of fruit flavonols. Fruit Research, 1, 1–11.

Xing M, Cao Y, Ren C, Liu Y, Li J, Grierson D, Martin C, Sun C, Chen K, Xu C, Li X. 2021b. Elucidation of myricetin biosynthesis in Morella rubra of the Myricaceae. The Plant Journal, 108, 411–425.

Yoshida K, Ma D, Constabel C P. 2015. The MYB182 protein down-regulates proanthocyanidin and anthocyanin biosynthesis in poplar by repressing both structural and regulatory flavonoid genes. Plant Physiology, 167, 693–710.

Zhang Q, Ge J, Liu X, Wang W, Liu X, Yin X. 2022. Consensus co-expression network analysis identifies AdZAT5 regulating pectin degradation in ripening kiwifruit. Journal of Advanced Research, 40, 59–68.

Zhang S, Zhu H. 2024. Development and prospect of gene-edited fruits and vegetables. Food Quality and Safety, 8, fyad045.

Zhang Y, De Stefano R, Robine M, Butelli E, Bulling K, Hill L, Rejzek M, Martin C, Schoonbeek H J. 2015. Different reactive oxygen species scavenging properties of flavonoids determine their abilities to extend the shelf life of tomato. Plant Physiology, 169, 1568-1583.

Zheng W, Wang H, Wang X, Li X, Hu J, Zi X, Zhou Y, Pan D, Fu Y. 2024. Kaempferol 3-O-rutinoside, a flavone derived from Tetrastigma hemsleyanum Diels et Gilg, reduces body temperature through accelerating the elimination of IL-6 and TNF-α in a mouse fever model. Molecules, 29, 1641.

 

[1] Niu Wang, Weidong Zhang, Zhenyu Zhong, Xiongbo Zhou, Xinran Shi, Xin Wang. FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3583-3597.
[2] Lichao Zhai, Shijia Song, Lihua Zhang, Jinan Huang, Lihua Lv, Zhiqiang Dong, Yongzeng Cui, Mengjing Zheng, Wanbin Hou, Jingting Zhang, Yanrong Yao, Yanhong Cui, Xiuling Jia. Subsoiling before winter wheat alleviates the kernel position effect of densely grown summer maize by delaying post-silking root–shoot senescence[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3384-3402.
[3] Tiantian Chen, Lei Li, Dan Liu, Yubing Tian, Lingli Li, Jianqi Zeng, Awais Rasheed, Shuanghe Cao, Xianchun Xia, Zhonghu He, Jindong Liu, Yong Zhang. Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3311-3321.
[4] Zuxian Chen, Bingbing Zhao, Yingying Wang, Yuqing Du, Siyu Feng, Junsheng Zhang, Luxiang Zhao, Weiqiang Li, Yangbao Ding, Peirong Jiao. H5N1 avian influenza virus PB2 antagonizes duck IFN-β signaling pathway by targeting mitochondrial antiviral signaling protein[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3614-3625.
[5] Yang Sun, Yu Liu, Li Zhou, Xinyan Liu, Kun Wang, Xing Chen, Chuanqing Zhang, Yu Chen. Activity of fungicide cyclobutrifluram against Fusarium fujikuroi and mechanism of the pathogen resistance associated with point mutations in FfSdhB, FfSdhC2 and FfSdhD[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3511-3528.
[6] Yufeng Xiao, Meiqi Dong, Xian Wu, Shuang Liang, Ranhong Li, Hongyu Pan, Hao Zhang. Enrichment, domestication, degradation, adaptive mechanism, and nicosulfuron bioremediation of bacteria consortium YM2[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3529-3545.
[7] Yuxin He, Fei Deng, Chi Zhang, Qiuping Li, Xiaofan Huang, Chenyan He, Xiaofeng Ai, Yujie Yuan, Li Wang, Hong Cheng, Tao Wang, Youfeng Tao. Wei Zhou, Xiaolong Lei, Yong Chen, Wanjun Ren. Can a delayed sowing date improve the eating and cooking quality of mechanically transplanted rice in the Sichuan Basin, China?[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3368-3383.
[8] Dili Lai, Md. Nurul Huda, Yawen Xiao, Tanzim Jahan, Wei Li, Yuqi He, Kaixuan Zhang, Jianping Cheng, Jingjun Ruan, Meiliang Zhou. Evolutionary and expression analysis of sugar transporters from Tartary buckwheat revealed the potential function of FtERD23 in drought stress[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3334-3350.
[9] Zishuai Wang, Wangchang Li, Zhonglin Tang. Enhancing the genomic prediction accuracy of swine agricultural economic traits using an expanded one-hot encoding in CNN models[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3574-3582.
[10] Yunji Xu, Xuelian Weng, Shupeng Tang, Weiyang Zhang, Kuanyu Zhu, Guanglong Zhu, Hao Zhang, Zhiqin Wang, Jianchang Yang. Untargeted lipidomic analysis of milled rice under different alternate wetting and soil drying irrigation regimes[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3351-3367.
[11] Minghui Li, Yilan Chen, Siqiao Wang, Xueke Sun, Yongkun Du, Siyuan Liu, Ruiqi Li, Zejie Chang, Peiyang Ding, Gaiping Zhang. Plug-and-display nanoparticle immunization of the core epitope domain induces potent neutralizing antibody and cellular immune responses against PEDV[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3598-3613.
[12] Jing Zhou, Bingshuai Du, Yibo Cao, Kui Liu, Zhihua Ye, Yiming Huang, Lingyun Zhang. Genome-wide identification of sucrose transporter genes in Camellia oleifera and characterization of CoSUT4[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3494-3510.
[13] Lixia Sheng, Yuqi Zhang, Xiaoke Yang, Yujia Yin, Jianqiang Yu. Functional differences between two homologous MYB transcription factors in regulating fruit color in octoploid strawberry (Fragaria×ananassa)[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3484-3493.
[14] Mingxin Feng, Ying Hu, Xin Yang, Jingwen Li, Haochen Wang, Yujia Liu, Haijun Ma, Kai Li, Jiayin Shang, Yulin Fang, Jiangfei Meng. Uncovering the miRNA-mediated regulatory network involved in postharvest senescence of grape berries[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3465-3483.
[15] Li Liu, Yifeng Feng, Ziqi Han, Yaxiao Song, Jianhua Guo, Jing Yu, Zidun Wang, Hui Wang, Hua Gao, Yazhou Yang, Yuanji Wang, Zhengyang Zhao. Functional analysis of the xyloglucan endotransglycosylase/hydrolase gene MdXTH2 in apple fruit firmness formation[J]. >Journal of Integrative Agriculture, 2025, 24(9): 3418-3434.
No Suggested Reading articles found!