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Journal of Integrative Agriculture
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Enrichment, domestication, degradation, adaptive mechanism, and nicosulfuron bioremediation of bacteria consortium YM2
Yufeng Xiao1, Meiqi Dong1, Xian Wu2, Shuang Liang1, Ranhong Li1, Hongyu Pan3, Hao Zhang1#

1 College of Plant Protection, Jilin Agricultural University, Changchun 130118, China

2 Institute of Plant Protection, Jilin Academy of Agricultural Sciences, ChangChun 130000, China

3 College of Plant Sciences, Jilin University, Changchun 130062, China

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摘要  烟嘧磺隆(NSR)是一种磺酰脲类除草剂,极易进入土壤和水体,影响自然环境和人类健康。本研究从某农药厂的活性淤泥中富集和驯化出降解菌群YM2,对烟嘧磺隆进行生物修复。对菌群YM2的培养基优化,筛选出最适碳源、氮源和无机盐分别为葡萄糖、酵母粉和NaCl,对其响应面分析,确定最佳培养条件为:9.41 g L1麦芽糊精、21.37 g L1酵母粉和12.45g L1NaCl,在此培养基下菌群YM2对烟嘧磺隆的降解率达97.49%优化菌群YM2对烟嘧磺隆的在不同环境条件下的降解特性,最佳降解条件为30℃pH 6.0菌群YM2接种量1%20 mg L1烟嘧磺隆初始浓度。并且,菌群YM2Cd2+Pb2+Ni2+ZN2+等重金属离子耐受,可以在重金属离子胁迫下生长和降解。菌群YM2主要通过胞外酶降解烟嘧磺隆,降解率92.17%。在降解过程中,细胞膜通透性、细胞表面疏水性活性氧含量、超氧化物歧化酶、过氧化氢酶、过氧化物酶、丙二醛含量、和细胞凋亡率均呈现先升高后降低的趋势,受到烟嘧磺隆胁迫时,激发菌群YM2的氧化应激系统,提升抗氧化酶活性,降低活性氧,并降解烟嘧磺隆,是菌群YM2的适应机制,即对烟嘧磺隆胁迫的应激反应。生物膜形成相关基因luxSwaaEspo0Awza的表达随时间和烟嘧磺隆浓度的变化而变化。适量的烟嘧磺隆诱导生物膜形成和激发氧化应激系统。污水和土壤中的20 mg kg1烟嘧磺隆分别被降至1.92 mg L12.72 mg L1,降解率达90.4%86.4%在模拟污水处理装置中水力停留时间12 h时第一阶段(前3 d)仅添加烟嘧磺隆,第二阶段(3-10 d)添加菌群YM210天后菌群YM2降解了84.55%的烟嘧磺隆。本研究为烟嘧磺隆的微生物修复提供了理论依据。

Abstract  Nicosulfuron (NSR), a sulfonylurea herbicide, can easily enter water bodies, affecting aquatic life and human safety. Here, bacteria consortium YM2 was enriched and domesticated from the active sludge of a pesticide plant for the bioremediation of NSR wastewater. The response surface methodology data revealed that under optimal incubation conditions: 9.41 g L1 maltodextrin, 21.37 g L1 yeast extract, and 12.45 g L1 NaCl, YM2 bacteria consortium degraded 97.49% of NSR within 4 d. The optimal degradation conditions were temperature 30°C, pH 6.0, inoculum 1%, and initial NSR concentration 20 mg L1), The degradation system was tolerant to heavy metal ions such as Cd2+, Pb2+, Ni2+, and Zn2+ and mainly occurred through the bacterial extracellular enzymes (92.17%). Mechanistically, during the degradation process, reactive oxygen species, oxidative stress, cell membrane permeability, cell surface hydrophobicity, and apoptosis rate first increased and then decreased. Also, the expression of biofilm formation-related genes luxS, waaE, spo0A, and wza varied with time and concentration. NSR wastewater and soil were degraded to 1.92 mg L1 and 2.72 mg L1, respectively. In a simulated wastewater treatment unit (hydraulic retention time 12 h), YM2 degraded 84.55% of NSR after 10 d. This study provides a theoretical basis for the microbial remediation of NSR.
Keywords:  Sulfonylurea herbicide       Bioremediation             Response surface methodology             Microbial degradation process  
Online: 21 March 2024  
About author:  Yufeng XIAO, Tel: +86-17649813417, E-mail: xyfethan@163.com,; #Correspondence Hao ZHANG, Tel: +86-13756097428, E-mail: haozhang100@163.com,

Cite this article: 

Yufeng Xiao, Meiqi Dong, Xian Wu, Shuang Liang, Ranhong Li, Hongyu Pan, Hao Zhang. 2024. Enrichment, domestication, degradation, adaptive mechanism, and nicosulfuron bioremediation of bacteria consortium YM2. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2024.03.004

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