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Journal of Integrative Agriculture  2017, Vol. 16 Issue (07): 1537-1546    DOI: 10.1016/S2095-3119(16)61595-2
Special Issue: 线虫合辑Nematology
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Golden Promise barley (Hordeum vulgare) is a suitable candidate model host for investigation interaction with Heterodera avenae
LUO Shu-jie1, 2, KONG Ling-an1, PENG Huan1, HUANG Wen-kun1, CUI Jiang-kuan1, LIU Jing1, QIAO Fen1, JIAN Heng2, PENG De-liang1
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 Key Laboratory of Plant Pathology, Ministry of Agriculture/College of Plant Protection, China Agricultural University, Beijing 100193, P.R.China
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Abstract      Heterodera avenae (cereal cyst nematode, CCN) infects many cereal crops and causes serious yield losses worldwide. Interaction studies investigating H. avenae and its hosts are still in their infancy. In this study, a barley model plant, the Hordeum vulgare cultivar Golden Promise, was investigated for its potential as a candidate model host to study its interaction with H. avenae. CCN-infective juveniles were attracted by the root tips and gathered around the root elongation zones of Golden Promise on 0.7% water agar plates. The juveniles invaded the roots and developed successfully until maturation at 40 days after inoculation in sterile sand soil. The cryotomy and syncytium measurements indicated that the syncytia enlarged gradually throughout the development of the nematodes and caused the corresponding root regions to swell obviously. Quantitative real-time PCR analysis showed that the down-regulation of defence-related barley genes and up-regulation of development-related barley genes contribute to the understanding of compatible interaction between H. avenae and Golden Promise. Barley stripe mosaic virus (BSMV) virus-induced gene silencing (VIGS) can be used in the roots of Golden Promise. In conclusion, the Hordeum vulgare cultivar Golden Promise is a suitable candidate model host for interaction studies with Heterodera avenae. The studies presented above document the first CCN host that not only has published genome context but also be compatible to BSMV VIGS.  
Keywords:  Golden Promise barley        Heterodera avenae        candidate model host        interaction  
Received: 13 February 2017   Accepted:

This project was supported by the National Natural Science Foundation of China (31571988), the Special Fund for Agro-scientific Research in the Public Interest, China (201503114), and the National Key Basic Research Program of China (973 Program, 2013CB127502).

Corresponding Authors:  Correspondence PENG De-liang, E-mail:; JIAN Heng, E-mail:   
About author:  LUO Shu-jie, E-mail:;

Cite this article: 

LUO Shu-jie, KONG Ling-an, PENG Huan, HUANG Wen-kun, CUI Jiang-kuan, LIU Jing, QIAO Fen, JIAN Heng, PENG De-liang . 2017. Golden Promise barley (Hordeum vulgare) is a suitable candidate model host for investigation interaction with Heterodera avenae. Journal of Integrative Agriculture, 16(07): 1537-1546.

Aditya J, Lewis J, Shirley N J, Tan H T, Henderson M, Fincher G B, Burton R A, Mather D E, Tucker M R. 2015. The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance. New Phytologist, 207, 135–147.

Bakker E, Dees R, Bakker J, Goverse A. 2006. Mechanisms involved in plant resistance to nematodes. In: Tuzun S, Bent E, eds., Multigenic and Induced Systemic Resistance in Plants. Springer, Boston, MA, US. pp. 314–334.

Chen S Y, Lang P, Chronis D, Zhang S, De Jong W S, Mitchum M G, Wang X H. 2015. In planta processing and glycosylation of a nematode CLAVATA3/ENDOSPERM SURROUNDING REGION-like effector and its interaction with a host CLAVATA2-like receptor to promote parasitism. Plant Physiology, 167, 262–272.

Chitwood D J. 2003. Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service. Pest Management Science, 59, 748–753.

Curtis R H C. 2008. Plant-nematode interactions: Environmental signals detected by the nematode’s chemosensory organs control changes in the surface cuticle and behaviour. Parasite, 15, 310–316.

Fudali S L, Wang C L, Williamson V M. 2013. Ethylene signaling pathway modulates attractiveness of host roots to the root-knot nematode Meloidogyne hapla. Molecular Plant-Microbe Interactions, 26, 75–86.

Gheysen G, Fenoll C. 2011. Arabidopsis as a tool for the study of plant-nematode interactions. In: Jones J, Gheysen G, Fenoll C, eds., Genomics and Molecular Genetics of Plant-Nematode Interactions. Springer, Dordrecht, the Netherlands. pp. 139–156.

Gheysen G, Mitchum M G. 2011. How nematodes manipulate plant development pathways for infection. Current Opinion in Plant Biology, 14, 415–421.

Giovanini M P, Puthoff D P, Nemacheck J A, Mittapalli O, Saltzmann K D, Ohm H W, Shukle R H, Williams C E. 2006. Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst. Molecular Plant-Microbe Interactions, 19, 1023–1033.

Grundler F M W, Sobczak M, Golinowski W. 1998. Formation of wall openings in root cells of Arabidopsis thaliana following infection by the plant-parasitic nematode Heterodera schachtii. European Journal of Plant Pathology, 104, 545–551.

Guo Y F, Ni J, Denver R, Wang X H, Clark S E. 2011. Mechanisms of molecular mimicry of plant CLE peptide ligands by the parasitic nematode Globodera rostochiensis. Plant Physiology, 157, 476–484.

Haegeman A, Mantelin S, Jones J T, Gheysen G. 2012. Functional roles of effectors of plant-parasitic nematodes. Gene, 492, 19–31.

Hamamouch N, Li C Y, Hewezi T, Baum T J, Mitchum M G, Hussey  R S, Vodkin L O, Davis E L. 2012. The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism. Journal of Experimental Botany, 63, 3683–3695.

Hewezi T. 2015. Cellular signaling pathways and posttranslational modifications mediated by nematode effector proteins. Plant Physiology, 169, 1018–1026.

Hewezi T, Howe P, Maier T R, Hussey R S, Mitchum M G, Davis E L, Baum T J. 2008. Cellulose binding protein from the parasitic nematode Heterodera schachtii interacts with Arabidopsis pectin methylesterase: Cooperative cell wall modification during parasitism. The Plant Cell, 20, 3080–3093.

Hiei Y, Ishida Y, Komari T. 2014. Progress of cereal transformation technology mediated by Agrobacterium tumefaciens. Frontiers in Plant Science, 5, 628.

Holzberg S, Brosio P, Gross C, Pogue G P. 2002. Barley stripe mosaic virus-induced gene silencing in a monocot plant. The Plant Journal, 30, 315–327.

Huang G Z, Dong R H, Allen R, Davis E L, Baum T J, Hussey R S. 2006. A root-knot nematode secretory peptide functions as a ligand for a plant transcription factor. Molecular Plant-Microbe Interactions, 19, 463–470.

INBGSC (International Barley Genome Sequencing Consortium), Mayer K F, Waugh R, Brown J W, Schulman A, Langridge P, Platzer M, Fincher G B, Muehlbauer G J, Sato K, Close T J, Wise R P, Stein N. 2012. A physical, genetic and functional sequence assembly of the barley genome. Nature, 491, 711–716.

Jayaprakash A, Rao Y S. 1982. Life history and behaviour of the cyst nematode, Heterodera oryzicola Rao and Jayaprakash, 1978 in rice (Oryza sativa L.). Proceedings of the Indian Academy of Sciences - Animal Sciences, 91, 283–295.

Jones J D, Dangl J L. 2006. The plant immune system. Nature, 444, 323–329.

Kammerhofer N, Radakovic Z, Regis J M A, Dobrev P, Vankova R, Grundler F M W, Siddique S, Hofmann J, Wieczorek K. 2015. Role of stress-related hormones in plant defence during early infection of the cyst nematode Heterodera schachtii in Arabidopsis. New Phytologist, 207, 778–789.

Kong L A, Wu D Q, Cui J K, Huang W K, Peng H, Peng D L. 2016. Testing and modelling the potential of three diploid plants in Poaceae as a new pathosystem to investigate the interactions between cereal hosts and cereal cyst nematode (Heterodera avenae). Plant Pathology, 65, 682–688.

Linsell K J, Riley I T, Davies K A, Oldach K H. 2014. Characterization of resistance to Pratylenchus thornei (Nematoda) in wheat (Triticum aestivum): Attraction, penetration, motility, and reproduction. Phytopathology, 104, 174–187.

Lozano-Torres J L, Wilbers R H P, Gawronski P, Boshoven J C, Finkers-Tomczak A, Cordewener J H G, America A H P, Overmars H A, Van ‘t Klooster J W, Baranowski L, Sobczak M, Ilyas M, Van Der Hoorn R, Schots A, De Wit  P J G M, Bakker J, Goverse A, Smant G. 2012. Dual disease resistance mediated by the immune receptor Cf-2 in tomato requires a common virulence target of a fungus and a nematode. Proceedings of the National Academy of Sciences of the United States of America, 109, 10119–10124.

Lozano-Torres J L, Wilbers R H P, Warmerdam S, Finkers-Tomczak A, Diaz-Granados A, Van Schaik C C, Helder J, Bakker J, Goverse A, Schots A, Smant G. 2014. Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors. PLOS Pathogens, 10, e1004569.

Matsumoto T, Tanaka T, Sakai H, Amano N, Kanamori H, Kurita K, Kikuta A, Kamiya K, Yamamoto M, Ikawa H, Fujii N, Hori K, Itoh T, Sato K. 2011. Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries. Plant Physiology, 156, 20–28.

Mcgrann G R D, Townsend B J, Antoniw J F, Asher M J C, Mutasa-Göttgens E S. 2008. Barley elicits a similar early basal defence response during host and non-host interactions with Polymyxa root parasites. European Journal of Plant Pathology, 123, 5–15.

Mitchum M G, Hussey R S, Baum T J, Wang X H, Elling A A, Wubben M, Davis E L. 2013. Nematode effector proteins: An emerging paradigm of parasitism. New Phytologist, 199, 879–894.

Mitchum M G, Wang X H, Davis E L. 2008. Diverse and conserved roles of CLE peptides. Current Opinion in Plant Biology, 11, 75–81.

Pacak A, Geisler K, Jørgensen B, Barciszewska-Pacak M, Nilsson L, Nielsen T H, Johansen E, Grønlund M, Jakobsen I, Albrechtsen M. 2010. Investigations of Barley stripe mosaic virus as a gene silencing vector in barley roots and in Brachypodium distachyon and oat. Plant Methods, 6, 26.

Patel N, Hamamouch N, Li C Y, Hewezi T, Hussey R S, Baum T J, Mitchum M G, Davis E L. 2010. A nematode effector protein similar to annexins in host plants. Journal of Experimental Botany, 61, 235–248.

Postma W J, Slootweg E J, Rehman S, Finkers-Tomczak A, Tytgat T O G, Van Gelderen K, Lozano-Torres J L, Roosien J, Pomp R, Van Schaik C, Bakker J, Goverse A, Smant G. 2012. The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants. Plant Physiology, 160, 944–954.

Rehman S, Postma W, Tytgat T, Prins P, Qin L, Overmars H, Vossen J, Spiridon L N, Petrescu A J, Goverse A, Bakker J, Smant G. 2009. A secreted SPRY Domain-Containing Protein (SPRYSEC) from the plant-parasitic nematode Globodera rostochiensis interacts with a CC-NB-LRR protein from a susceptible tomato. Molecular Plant-Microbe Interactions, 22, 330–340.

Yuan C, Li C, Yan L, Jackson AO, Liu Z, Han C, Yu J, Li D. 2011. A high throughput Barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots. PLoS ONE, 6, e26468. 
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