Please wait a minute...
Journal of Integrative Agriculture  2017, Vol. 16 Issue (01): 190-198    DOI: 10.1016/S2095-3119(15)61308-9
Soil & Fertilization﹒Irrigation﹒Plant Nutrition﹒ Agro-Ecology & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Nitrogen (N) metabolism related enzyme activities, cell ultrastructure and nutrient contents as affected by N level and barley genotype
Jawad Munawar Shah, Syed Asad Hussain Bukhari, Zeng Jian-bin, Quan Xiao-yan, Essa Ali, Noor Muhammad, Zhang Guo-ping

Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou 310058, P.R.China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  Development of the new crop cultivars with high yield under low nitrogen (N) input is a fundamental approach to enhance agricultural sustainability, which is dependent on the exploitation of the elite germplasm.  In the present study, four barley genotypes (two Tibetan wild and two cultivated), differing in N use efficiency (NUE), were characterized for their physiological and biochemical responses to different N levels.  Higher N levels significantly increased the contents of other essential nutrients (P, K, Ca, Fe, Cu and Mn), and the increase was more obvious for the N-efficient genotypes (ZD9 and XZ149).  The observation of ultrastructure showed that chloroplast structure was severely damaged under low nitrogen, and the two high N efficient genotypes were relatively less affected.  The activities of the five N metabolism related enzymes, i.e.,  nitrate reductase (NR), glutamine synthetase (GS), nitrite reductase (NiR), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) all showed the substantial increase with the increased N level in the culture medium.  However the increased extent differed among the four genotypes, with the two N efficient genotypes showing more increase in comparison with the other two genotypes with relative N inefficiency (HXRL and XZ56).  The current findings showed that a huge difference exists in low N tolerance among barley genotypes, and improvement of some physiological traits (such as enzymes) could be helpful for increasing N utilization efficiency. 
Keywords:  barley      enzyme      genotype      nitrogen      ultrastructure  
Received: 09 November 2015   Online: 21 January 2016   Accepted:
Fund: 

This research was supported by the National Natural Science Foundation of China (31330055), the China Agriculture Research System (CARS-05) and the Jiangsu Collaborative Innovation Center for Modern Crop Production, China (JCIC-MCP).

Corresponding Authors:  ZHANG Guo-ping, Tel: +86-571-88982115, E-mail: zhanggp@zju.edu.cn    
About author:  Jawad Munawar Shah, E-mail: shah8712@yahoo.com

Cite this article: 

Jawad Munawar Shah, Syed Asad Hussain Bukhari, ZENG Jian-bin, QUAN Xiao-yan, Essa Ali, Noor Muhammad, ZHANG Guo-ping . 2017. Nitrogen (N) metabolism related enzyme activities, cell ultrastructure and nutrient contents as affected by N level and barley genotype. Journal of Integrative Agriculture, 16(01): 190-198.

Abdel-Ghani A H, Kumar B, Reyes-Matamoros J. 2013. Genotypic variation and relationships between seedling and adult plant traits in maize (Zea mays L.) inbred lines grown under contrasting nitrogen levels. Euphytica, 189, 123–133.

Anbessa Y, Juskiw P, Good A, Nyachiro J, Helm J. 2009. Genetic variability in nitrogen use efficiency of spring barley. Crop Science, 49, 1259–1269.

Bhaskar R, Syvertsen P J. 2005. Concurrent changes in net CO2 assimilation and chloroplast structure in nitrogen deficient citrus leaves. Environment and Experimental Botany, 54, 41–48.

Britto D T, Kronzucker H J. 2002. NH4+ toxicity in higher plants: a critical review. Journal of Plant Physiology, 159, 567–584.

Bukhari S A H, Shang S H, Zhang M, Zheng W T, Zhang G P, Wang T Z, Shamsi I H, Wu F B. 2015. Genome-wide identification of chromium stress responsive micro RNAs and their target genes in tobacco (Nicotiana tabacum) roots. Environment and Toxicology Chemestry, 34, 2573–2582.

Cheng S Z, Jie S Y. 1995. Study of effect of N fertilizer on Zn, Mn, Fe, Mg content of barley. Chinese Journal Soil Science, 30, 71–73. (in Chinese)

Crawford N M, Arst H N. 1993. The molecular genetics of nitrate assimilation in fungi and plants. Annual Review on Genetics, 27, 115–146.

Cren M, Hirel B. 1999. Glutamine synthetase in higher plants: Regulation of gene and protein expression from the organ to the cell. Plant and Cell Physiology, 40, 1187–1193.

Dai F, Nevo E, Wu D, Comadran J, Zhou M, Qiu L, Chen Z, Beiles A, Chen G, Zhang G. 2012. Tibet is one of the centers of domestication of cultivated barley. Proceedings of the National Academy of Sciences of the United States of America, 109, 16969–16973.

Dawson J C, Huggins D R, Jones S S. 2008. Characterizing nitrogen use efficiency to improve crop performance in organic and sustainable agricultural systems. Field Crops Research, 107, 89–101.

Dubois F, Terce-Laforgue B M, Gonzalez-Moro, Estavillo M J, Sangwan R, Gallais A, Hirel B. 2003. Glutamate dehydrogenase in plants: Is there a new story for an old enzyme? Plant Physiology and  Biochemistry, 41, 565–576.

Ellis R P, Forster B P, Robinson D, Handley L L, Gordon D C, Russell J R, Powell W. 2000. Wild barley: A source of genes for crop improvement in the 21st century. Journal of Experimental Botany, 51, 9–17.

Fageria V D. 2001. Nutrient interactions in crop plants. Journal of Plant Nutrition, 24, 1269–1290.

Feng Z Y, Zhang L L, Zhang Y Z, Ling H Q. 2006. Genetic diversity and geographical differentiation of cultivated six-rowed naked barley landraces from the Qinghai-Tibet plateau of China detected by SSR analysis. Genetic and Molecular Biology, 29, 330–338.

Good A G, Shrawat A K, Muench D G. 2004. Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production. Trends in Plant Science, 9, 597–605.

Gregorio G B, Senadhira D, Htut H, Graham R D. 2000. Breeding for trace mineral density in rice. Food Nutrition Bulletin, 21, 382–386.

Grotz N, Guerinot M L. 2006. Molecular aspects of Cu, Fe and Zn homeostasis in plants. Biochimica et Biophysica Acta, 1763, 595–608.

Grub A, Machler F. 1990. Photosynthesis and light activation of ribulose 1,5-bisphosphate carboxylase in the presence of starch. Journal  of Experimental Botany, 41, 1293–1301.

Guo J H, Liu X J, Zhang Y, Shen J L, Han W X, Zhang W F, Christie P, Goulding K W T, Vitousek P M, Zhang F S. 2010. Significant acidification in major Chinese croplands. Science, 327, 1008–1010.

Gupta N, Gupta A K, Gaur V S, Kumar A. 2012. Relationship of nitrogen use efficiency with the activities of enzymes involved in nitrogen uptake and assimilation of finger millet genotypes grown under different nitrogen inputs. Scientific World Journal, doi: 10.1100/2012/625731

Hageman R H, Hucklesby D P. 1971. Nitrate reductase. Methods of Enzymology, 23, 497–503.

Hakeem K R, Ahmad A, Iqbal M, Gucel S, Ozturk M. 2011. Nitrogen-efficient rice cultivars can reduce nitrate pollution. Environmental Science and Pollution Research, 18, 1184–1193.

Husted S, Mattsson M, Mollers C, Wallbraun M, Schjoerring J K. 2002. Photorespiratory NH4+ production in leaves of wild-type and glutamine synthetase 2 antisense oilseed rape. Plant Physiology, 130, 989–998.

Ikram S, Bedu M, Daniel-Vedele F, Chaillou S, Chardon F. 2012. Natural variation of Arabidopsis response to nitrogen availability. Journal of Experimental Botany, 63, 91–105.

Kemp A. 1983. The effects of fertilizer treatment of grassland on the biological availability of magnesium to ruminants. In: Fontenot J P, Bunce G E, Webb K E, Allen V G, eds., Role of Magnesium in Animal Nutrition. VPISU, Blacksburg, VA. pp. 143–157.

Kessel B, Schierholt A, Becker H C. 2012. Nitrogen use efficiency in a genetically diverse set of winter oilseed rape (Brassica napus L.). Crop Science, 52, 2546–2554.

Lea P J, Ireland R J. 1999. Nitrogen metabolism in higher plants. In: Singh B K, ed., Plant Amino Acids, Biochemistry and Biotechnology. Marcel Dekker, New York. pp. 1–4.

Lea P J, Leegood R C. 1993. Nitrogen metabolism. In: Lea P J, Leegood R C, eds., Plant Biochemistry and Molecular Biology. Wiley, New York, USA. pp. 155–180.

Liu X, Zhang Y, Han W, Tang A H, Shen J, Cui Z, Vitousek P, Erisman J W, Goulding K, Christie P, Fangmeier A, Zhang F. 2013. Enhanced nitrogen deposition over China. Nature, 494, 459–462.

Masclaux C, Quillere I, Gallis A, Hirel B. 2001. The challenge of remobilization in plant nitrogen economy: A survey of physioagronomic and molecular approaches. Annals of Applied Biology, 138, 69–81.

Najeeb U, Xu L, Ali S, Jilani G, Gong H J, Shen W Q, Zhou W J. 2009. Citric acid enhances the phytoextraction of manganese and plant growth by alleviating the ultra-structural damages in Juncus effuses L. Journal of  Hazardous Material, 170, 115–1163.

Nevo E. 2005. Population genetic structure of wild barley and wheat in the Near East Fertile Crescent: regional and local adaptive patterns. In: Gupta P K, Varshney R K, eds., Cereal Genome. Springer, Netherlands. pp. 135–163.

O’Neal D, Joy K W. 1973. Glutamine synthetase of pea leaves: Purification, stabilization and pH optima. Archive of Biochemistry and  Biophysics, 159, 113–122.

Peoples M B, Gifford R M. 1993. Long-distance transport of carbon and nitrogen from sources to sinks in higher plants. In: Dennis D T, Turpin D H, eds., Biochemistry and Molecular Biology. Wiley, New York, USA. pp. 434–447.

Shi W M, Xu W F, Li S M, Zhao X Q, Dong G Q. 2010. Responses of two rice cultivars differing in seedling-stage nitrogen use efficiency to growth under low-nitrogen conditions. Plant and Soil, 326, 291–302.

Silveira J A G, Matos J C S, Cecatto V M, Viegas R A, Oliveira J T A. 2001. Nitrate reductase ctivity, distribution, and response to nitrate in two contrasting Phaseolus species inoculated with Rhizobium spp. Environmental and Experimental Botany, 46, 37–46.

Sumner M E, Farina M P W. 1986. Phosphorus interactions with other nutrients and lime in field cropping systems. Advance in Soil Science, 5, 201–236.

Terashima I J, Evans J R. 1988. Effects of light and nitrogen nutrition on the organization of the photosynthetic apparatus in spinach. Plant and Cell Physiology, 29, 143–155.

Vijayalakshmi P, Vishnukiran T, Ramana K B, Srikanth B, Subhakar R I, Swamy K N, Surekha K, Sailaja N, Subbarao L V, Raghuveer R P, Subrahmanyam D, Neeraja C N, Voleti S R. 2015. Biochemical and physiological characterization for nitrogen use efficiency in aromatic rice genotypes. Field Crops Research, 179, 132–143.

Vincentz M, Moureaux T, Leydecker M T, Vaucheret H, Caboche M. 1993. Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites. The Plant Journal, 3, 315–324.

Wang A, Yu Z, Ding Y. 2009. Genetic diversity analysis of wild close relatives of barley from Tibet and the Middle East by ISSR and SSR markers. Biologies, 332, 393–403.

Wei D, Cui K, Ye G, Pan J, Xiang J, Huang J, Nie L, Estavillo J M. 2012. QTL mapping for nitrogen-use efficiency and nitrogen-deficiency tolerance traits in rice. Plant and Soil, 359, 281–295.

Wilkinson S R, Grunes D L, Sumner M E. 1999. Nutrient interactions in soil and plant nutrition. In: Sumner M E, ed., Handbook of Soil Science. CRC Press, Boca Raton, FL. pp. 89–112.

Xu G, Fan X, Miller A J. 2012. Plant nitrogen assimilation and use efficiency. Annual Review on Plant Biology, 63, 153–182.

Yan J, Chen G, Cheng J, Nevo E, Gutterman Y. 2008. Phenotypic variation in caryopsis dormancy and seedling salt tolerance in wild barley, Hordeum spontaneum, from different habitats in Israel. Genetic Resource and Crop Evolution, 55, 995–1005.

Ye X, Hong J, Shi L, Xu F. 2010. Adaptability mechanism of nitrogen-efficient germplasm of natural variation to low nitrogen stress in Brassica napus. Journal of Plant Nutrition, 33, 2028–2040.
 
[1] Gaozhao Wu, Xingyu Chen, Yuguang Zang, Ying Ye, Xiaoqing Qian, Weiyang Zhang, Hao Zhang, Lijun Liu, Zujian Zhang, Zhiqin Wang, Junfei Gu, Jianchang Yang. An optimized strategy of nitrogen-split application based on the leaf positional differences in chlorophyll meter readings[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2605-2617.
[2] Sainan Geng, Lantao Li, Yuhong Miao, Yinjie Zhang, Xiaona Yu, Duo Zhang, Qirui Yang, Xiao Zhang, Yilun Wang. Nitrogen rhizodeposition from corn and soybean, and its contribution to the subsequent wheat crops[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2446-2457.
[3] Wenjie Yang, Jie Yu, Yanhang Li, Bingli Jia, Longgang Jiang, Aijing Yuan, Yue Ma, Ming Huang, Hanbing Cao, Jinshan Liu, Weihong Qiu, Zhaohui Wang. Optimized NPK fertilizer recommendations based on topsoil available nutrient criteria for wheat in drylands of China[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2421-2433.
[4] Hanzhu Gu, Xian Wang, Minhao Zhang, Wenjiang Jing, Hao Wu, Zhilin Xiao, Weiyang Zhang, Junfei Gu, Lijun Liu, Zhiqin Wang, Jianhua Zhang, Jianchang Yang, Hao Zhang.

The response of roots and the rhizosphere environment to integrative cultivation practices in paddy rice [J]. >Journal of Integrative Agriculture, 2024, 23(6): 1879-1896.

[5] Ping Chen, Qing Du, Benchuan Zheng, Huan Yang, Zhidan Fu, Kai Luo, Ping Lin, Yilin Li, Tian Pu, Taiwen Yong, Wenyu Yang.

Coordinated responses of leaf and nodule traits contribute to the accumulation of N in relay intercropped soybean [J]. >Journal of Integrative Agriculture, 2024, 23(6): 1910-1928.

[6] Shuo Yuan, Ruonan Li, Yinjie Zhang, Hao'an Luan, Jiwei Tang, Liying Wang, Hongjie Ji, Shaowen Huang.

Effects of long-term partial substitution of inorganic fertilizer with pig manure and/or straw on nitrogen fractions and microbiological properties in greenhouse vegetable soils [J]. >Journal of Integrative Agriculture, 2024, 23(6): 2083-2098.

[7] Yuguang Zang, Gaozhao Wu, Qiangqiang Li, Yiwen Xu, Mingming Xue, Xingyu Chen, Haiyan Wei, Weiyang Zhang, Hao Zhang, Lijun Liu, Zhiqin Wang, Junfei Gu, Jianchang Yang.

Irrigation regimes modulate non-structural carbohydrate remobilization and improve grain filling in rice (Oryza sativa L.) by regulating starch metabolism [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1507-1522.

[8] Zhao Liu, Yuan Gao, Kun Wang, Jianrong Feng, Simiao Sun, Xiang Lu, Lin Wang, Wen Tian, Guangyi Wang, Zichen Li, Qingshan Li, Lianwen Li, Dajiang Wang.

Identification of S-RNase genotype and analysis of its origin and evolutionary patterns in Malus plants [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1205-1221.

[9] Junnan Hang, Bowen Wu, Diyang Qiu, Guo Yang, Zhongming Fang, Mingyong Zhang.

OsNPF3.1, a nitrate, abscisic acid and gibberellin transporter gene, is essential for rice tillering and nitrogen utilization efficiency [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1087-1104.

[10] Yongjian Chen, Lan Dai, Siren Cheng, Yong Ren, Huizi Deng, Xinyi Wang, Yuzhan Li, Xiangru Tang, Zaiman Wang, Zhaowen Mo.

Regulation of 2-acetyl-1-pyrroline and grain quality in early-season indica fragrant rice by nitrogen and silicon fertilization under different plantation methods [J]. >Journal of Integrative Agriculture, 2024, 23(2): 511-535.

[11] Ping’an Zhang, Mo Li, Qiang Fu, Vijay P. Singh, Changzheng Du, Dong Liu, Tianxiao Li, Aizheng Yang.

Dynamic regulation of the irrigation–nitrogen–biochar nexus for the synergy of yield, quality, carbon emission and resource use efficiency in tomato [J]. >Journal of Integrative Agriculture, 2024, 23(2): 680-697.

[12] Qiuyan Yan, Linjia Wu, Fei Dong, Shuangdui Yan, Feng Li, Yaqin Jia, Jiancheng Zhang, Ruifu Zhang, Xiao Huang.

Subsoil tillage enhances wheat productivity, soil organic carbon and available nutrient status in dryland fields [J]. >Journal of Integrative Agriculture, 2024, 23(1): 251-266.

[13] Akmaral Baidyussen, Gulmira Khassanova, Maral Utebayev, Satyvaldy Jatayev, Rystay Kushanova, Sholpan Khalbayeva, Aigul Amangeldiyeva, Raushan Yerzhebayeva, Kulpash Bulatova, Carly Schramm, Peter Anderson, Colin L. D. Jenkins, Kathleen L. Soole, Yuri Shavrukov. Assessment of molecular markers and marker-assisted selection for drought tolerance in barley (Hordeum vulgare L.)[J]. >Journal of Integrative Agriculture, 2024, 23(1): 20-38.
[14] Qiao Li, Jianying Guo, Han Zhang, Mengxin Zhao.

The competition between Bidens pilosa and Setaria viridis alters soil microbial composition and soil ecological function [J]. >Journal of Integrative Agriculture, 2024, 23(1): 267-282.

[15] Xiaotong Guo, Xiangju Li, Zheng Li, Licun Peng, Jingchao Chen, Haiyan Yu, Hailan Cui. Effect of mutations on acetohydroxyacid synthase (AHAS) function in Cyperus difformis L.[J]. >Journal of Integrative Agriculture, 2024, 23(1): 177-186.
No Suggested Reading articles found!