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Journal of Integrative Agriculture  2022, Vol. 21 Issue (12): 3626-3636    DOI: 10.1016/j.jia.2022.08.012
Special Issue: 农业生态环境-肥料及施用合辑Agro-ecosystem & Environment—Fertilizer
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Characteristics of inorganic phosphorus fractions and their correlations with soil properties in three non-acidic soils

ZHANG Nai-yu1, WANG Qiong1, 2, ZHAN Xiao-ying3, WU Qi-hua4, HUANG Shao-min5, ZHU Ping6, YANG Xue-yun7, ZHANG Shu-xiang1

1 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land, Beijing 100081, P.R.China

2 TERRA, Gembloux Agro-Bio Tech, University of Liege, Gembloux 5030, Belgium

3 Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

4 Institute of Bioengineering, Guangdong Academy of Sciences/Guangdong Modern Agricultural Technology Research and Development Center, Guangzhou 510316, P.R.China

5 Institute of Plant Nutrient, Agricultural Resources and Environmental Sciences, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China

6 Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun 130033, P.R.China

7 College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, P.R.China

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Understanding the characteristics and influences of various factors on phosphorus (P) fractions is of significance for promoting the efficiency of soil P.  Based on long-term experiments on black soil, fluvo-aquic soil, and loess soil, which belong to Phaeozems, Cambisols, and Anthrosols in the World Reference Base for Soil Resources (WRB), respectively, five fertilization practices were selected and divided into three groups: no P fertilizer (CK/NK), balanced fertilizer (NPK/NPKS), and manure plus mineral fertilizer (NPKM).  Soil inorganic P (Pi) fractions and soil properties were analyzed to investigate the characteristics of the Pi fractions and the relationships between Pi fractions and various soil properties.  The results showed that the proportion of Ca10-P in the sum of total Pi fractions was the highest in the three soils, accounting for 33.5% in black soil, 48.8% in fluvo-aquic soil, and 44.8% in loess soil.  Long-term fertilization practices resulted in periodic changes in soil Pi accumulation or depletion.  For black soil and fluvo-aquic soil, the Pi accumulation was higher in the late period (10–20 years) of fertilization than in the early period (0–10 years) under NPK/NPKS and NPKM, whereas the opposite result was found in loess soil.  The Pi accumulation occurred in all Pi fractions in black soil; mainly in Ca8-P, Fe-P, and Ca10-P in fluvo-aquic soil; and in Ca2-P, Ca8-P, and O-P in loess soil.  Under CK/NK, the soil Pi was depleted mainly in the early period in each of the three soils.  In addition to the labile Pi (Ca2-P) and moderately labile Pi (Ca8-P, Fe-P, Al-P), the Ca10-P in black soil and fluvo-aquic soil and O-P in loess soil could also be used by crops.  Redundancy analysis showed that soil properties explained more than 90% of the variation in the Pi fractions in each soil, and the explanatory percentages of soil organic matter (SOM) were 43.6% in black soil, 74.6% in fluvo-aquic, and 38.2% in loess soil.  Consequently, decisions regarding the application of P fertilizer should consider the accumulation rate and the variations in Pi fractions driven by soil properties in non-acidic soils.

Keywords:  non-acidic soils       long-term fertilization              phosphorus fractions              soil properties              organic matter  
Received: 07 December 2021   Accepted: 09 May 2022
Fund: This research was supported by the National Key Research and Development Program of China (2021YFD1500205) and the National Natural Science Foundation of China (41977103).
About author:  ZHANG Nai-yu, E-mail:; Correspondence ZHANG Shu-xiang, Tel: +86-10-82106202, E-mail:

Cite this article: 

ZHANG Nai-yu, WANG Qiong, ZHAN Xiao-ying, WU Qi-hua, HUANG Shao-min, ZHU Ping, YANG Xue-yun, ZHANG Shu-xiang. 2022. Characteristics of inorganic phosphorus fractions and their correlations with soil properties in three non-acidic soils. Journal of Integrative Agriculture, 21(12): 3626-3636.

Andersson K O, Tighe M K, Guppy C N, Milham P J, McLaren T I. 2015. Incremental acidification reveals phosphorus release dynamics in alkaline vertic soils. Geoderma, 259, 35–44.
Audette Y, Smith D S, Parsons C T, Chen W B, Rezanezhad F, Van Cappellen P. 2020. Phosphorus binding to soil organic matter via ternary complexes with calcium. Chemosphere, 260, 127624.
Bao Y P, Bolan N S, Lai J H, Wang Y S, Jin X H, Kirkham M B, Wu X L, Fang Z, Zhang Y, Wang H L. 2021. Interactions between organic matter and Fe (hydr)oxides and their influences on immobilization and remobilization of metal(loid)s: A review. Critical Reviews in Environmental Science and Technology, 9, 1–22.
Chen M M, Zhang S R, Liu L, Wu L P, Ding X D. 2021. Combined organic amendments and mineral fertilizer application increase rice yield by improving soil structure, P availability and root growth in saline-alkaline soil. Soil and Tillage Research, 212, 105060. 
Chen M P, Graedel T E. 2016. A half-century of global phosphorus flows, stocks, production, consumption, recycling, and environmental impacts. Global Environmental Change, 36, 139–152. 
Chen S, Cade-Menun B J, Bainard L D, St Luce M, Hu Y F, Chen Q. 2021. The influence of long-term N and P fertilization on soil P forms and cycling in a wheat/fallow cropping system. Geoderma, 404, 115274. 
Cooper J, Lombardi R, Boardman D, Carliell-Marquet C. 2011. The future distribution and production of global phosphate rock reserves. Resources Conservation and Recycling, 57, 78–86.
Deiss L, de Moraes A, Maire V. 2018. Environmental drivers of soil phosphorus composition in natural ecosystems. Biogeosciences, 15, 4575–4592.
Eriksson A K, Gustafsson J P, Hesterberg D. 2015. Phosphorus speciation of clay fractions from long-term fertility experiments in Sweden. Geoderma, 241, 68–74.
Eriksson A K, Hillier S, Hesterberg D, Klysubun W, Ulen B, Gustafsson J P. 2016. Evolution of phosphorus speciation with depth in an agricultural soil profile. Geoderma, 280, 29–37.
Fink J R, Inda A V, Tiecher T, Barron V. 2016. Iron oxides and organic matter on soil phosphorus availability. Ciencia e Agrotecnologia, 40, 369–379.
Gatiboni L C, Schmitt D E, Tiecher T, Veloso M G, dos Santos D R, Kaminski J, Brunetto G. 2021. Plant uptake of legacy phosphorus from soils without P fertilization. Nutrient Cycling in Agroecosystems, 119, 139–151.
Ge X F, Wang L J, Zhang W J, Putnis C V. 2020. Molecular understanding of humic acid-limited phosphate precipitation and transformation. Environmental Science and Technology, 54, 207–215.
Hansen J C, Cade-Menun B J, Strawn D G. 2004. Phosphorus speciation in manure-amended alkaline soils. Journal of Environmental Quality, 33, 1521–1527.
Helfenstein J, Tamburini F, von Sperber C, Massey M S, Pistocchi C, Chadwick O A, Vitousek P M, Kretzschmar R, Frossard E. 2018. Combining spectroscopic and isotopic techniques gives a dynamic view of phosphorus cycling in soil. Nature Communications, 9, 3226. 
Jiang B F, Gu Y C. 1989. A suggested fractionation scheme of inorganic phosphorus in calcareous soils. Scientia Agricultura Sinica, 22, 58–66. (in Chinese)
Jiao X Q, Lyu Y, Wu X B, Li H G, Cheng L Y, Zhang C C, Yuan L X, Jiang R F, Jiang B W, Rengel Z, Zhang F S, Davies W J, Shen J B. 2016. Grain production versus resource and environmental costs: towards increasing sustainability of nutrient use in China. Journal of Experimental Botany, 67, 4935–4949.
Khan A, Lu G Y, Ayaz M, Zhang H T, Wang R J, Lv F L, Yang X Y, Sun B H, Zhang S L. 2018. Phosphorus efficiency, soil phosphorus dynamics and critical phosphorus level under long-term fertilization for single and double cropping systems. Agriculture Ecosystems and Environment, 256, 1–11.
Khosravi A, Zarei M, Ronaghi A. 2017. Influence of biofertilizers and phosphate sources on the phosphorus uptake of lettuce and chemical forms of phosphorus in soil. Communications in Soil Science and Plant Analysis, 48, 2701–2714.
Li F Y, Zhang Q, Klumpp E, Bol R, Nischwitz V, Ge Z, Liang X Q. 2021. Organic carbon linkage with soil colloidal phosphorus at regional and field scales: Insights from size fractionation of fine particles. Environmental Science and Technology, 55, 5815–5825.
Li H, Huang G, Meng Q, Ma L, Yuan L, Wang F, Zhang W, Cui Z, Shen J, Chen X, Jiang R, Zhang F. 2011. Integrated soil and plant phosphorus management for crop and environment in China. A review. Plant and Soil, 349, 157–167. 
Li J Q, Nie M, Pendall E. 2020. Soil physico-chemical properties are more important than microbial diversity and enzyme activity in controlling carbon and nitrogen stocks near Sydney, Australia. Geoderma, 366, 114201.
Lin J W, Zhao Y Y, Zhan Y H, Wang Y. 2020. Influence of coexisting calcium and magnesium ions on phosphate adsorption onto hydrous iron oxide. Environmental Science and Pollution Research, 27, 11303–11319.
Liu J, Han C Q, Zhao Y H, Yang J J, Cade-Menun B J, Hu Y F, Li J M, Liu H, Sui P, Chen Y Q, Ma Y B. 2020. The chemical nature of soil phosphorus in response to long-term fertilization practices: Implications for sustainable phosphorus management. Journal of Cleaner Production, 272, 123093.
Liu J, Yang J J, Cade-Menun B J, Hu Y F, Li J M, Peng C, Ma Y B. 2017. Molecular speciation and transformation of soil legacy phosphorus with and without long-term phosphorus fertilization: Insights from bulk and microprobe spectroscopy. Scientific Reports, 7, 15354.
Liu Y T, Hesterberg D. 2011. Phosphate bonding on noncrystalline Al/Fe-hydroxide coprecipitates. Environmental Science and Technology, 45, 6283–6289.
Lu R K. 1999. Soil and Agro-Chemistry Analytical Method. China Agricultural Science and Technology Press, Beijing. (in Chinese)
Ma J, Ma Y L, Wei R F, Chen Y L, Weng L P, Ouyang X X, Li Y T. 2021. Phosphorus transport in different soil types and the contribution of control factors to phosphorus retardation. Chemosphere, 276, 130012.
Ma Y L, Ma J, Peng H, Weng L P, Chen Y L, Li Y T. 2019. Effects of iron, calcium, and organic matter on phosphorus behavior in fluvo-aquic soil: Farmland investigation and aging experiments. Journal of Soils and Sediments, 19, 3994–4004.
MacDonald G K, Bennett E M, Potter P A, Ramankutty N. 2011. Agronomic phosphorus imbalances across the world’s croplands. Proceedings of the National Academy of Sciences of the United States of America, 108, 3086–3091.
Mehlich A. 1984. Mehlich 3 soil test extractant: A modification of Mehlich 2 extractant. Communications in Soil Science and Plant Analysis, 15, 1409–1416.
Mehra O P, Jackson M L. 1960. Iron oxides removal from soil and clays by a dithionite-citrate system buffered with sodium bicarbonate. Clays and Clay Minerals, 7, 317–327.
Meyer G, Bell M J, Lombi E, Doolette C L, Brunetti G, Novotny E H, Klysubun W, Zhang Y Q, Kopittke P M. 2021. Phosphorus speciation in the fertosphere of highly concentrated fertilizer bands. Geoderma, 403, 115208.
Murphy J, Riley J P. 1962. A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta, 27, 31–36.
De Oliveira L E Z, Nunes R D, de Sousa D M G, de Figueiredo C C. 2020. Dynamics of residual phosphorus forms under different tillage systems in a Brazilian Oxisol. Geoderma, 367, 114254.
Pizzeghello D, Berti A, Nardi S, Morari F. 2011. Phosphorus forms and P-sorption properties in three alkaline soils after long-term mineral and manure applications in north-eastern Italy. Agriculture Ecosystems and Environment, 141, 58–66.
Sato S, Solomon D, Hyland C, Ketterings Q M, Lehmann J. 2005. Phosphorus speciation in manure and manure-amended soils using XANES spectroscopy. Environmental Science and Technology, 39, 7485–7491.
Schmieder F, Bergstrom L, Riddle M, Gustafsson J P, Klysubun W, Zehetner F, Condron L, Kirchmann H. 2018. Phosphorus speciation in a long-term manure-amended soil profile - Evidence from wet chemical extraction, 31P-NMR and P K-edge XANES spectroscopy. Geoderma, 322, 19–27.
Schubert S, Steffens D, Ashraf I. 2020. Is occluded phosphate plant-available? Journal of Plant Nutrition and Soil Science, 183, 338–344.
Shen P, Xu M, Zhang H, Yang X, Huang S, Zhang S, He X. 2014. Long-term response of soil Olsen P and organic C to the depletion or addition of chemical and organic fertilizers. Catena, 118, 20–27.
Shen Y, Duan Y H, McLaughlin N, Huang S M, Guo D D, Xu M H. 2019. Phosphorus desorption from calcareous soils with different initial Olsen-P levels and relation to phosphate fractions. Journal of Soils and Sediments, 19, 2997–3007.
Tunesi S, Poggi V, Gessa C. 1999. Phosphate adsorption and precipitation in calcareous soils: the role of calcium ions in solution and carbonate minerals. Nutrient Cycling in Agroecosystems, 53, 219–227.
Wang H, Zhu J, Fu Q L, Hong C, Hu H Q, Violante A. 2016. Phosphate adsorption on uncoated and humic acid-coated iron oxides. Journal of Soils and Sediments, 16, 1911–1920.
Wang X, Phillips B L, Boily J F, Hu Y, Hu Z, Yang P, Feng X, Xu W, Zhu M. 2019. Phosphate sorption speciation and precipitation mechanisms on amorphous aluminum hydroxide. Soil Systems, 3, 20.
Wang Y, Bauke S L, von Sperber C, Tamburini F, Guigue J, Winkler P, Kaiser K, Honermeier B, Amelung W. 2021. Soil phosphorus cycling is modified by carbon and nitrogen fertilization in a long-term field experiment. Journal of Plant Nutrition and Soil Science, 184, 282–293.
Wang Z C, Huang S, Li D H. 2019. Decomposition of cyanobacterial bloom contributes to the formation and distribution of iron-bound phosphorus (Fe-P): Insight for cycling mechanism of internal phosphorus loading. Science of the Total Environment, 652, 696–708. 
Weihrauch C, Opp C. 2018. Ecologically relevant phosphorus pools in soils and their dynamics: The story so far. Geoderma, 325, 183–194.
Wu Q H, Zhang S X, Zhu P, Huang S M, Wang B R, Zhao L P, Xu M G. 2017. Characterizing differences in the phosphorus activation coefficient of three typical cropland soils and the influencing factors under long-term fertilization. PLoS ONE, 12, e0176437. 
Yadav B K, Verma A. 2012. Phosphate solubilization and mobilization in soil through microorganisms under arid ecosystems. The Functioning of Ecosystems, 27, 93–108.
Yamamoto K, Hashimoto Y, Kang J, Kobayashi K. 2018. Speciation of phosphorus zinc and copper in soil and water dispersible colloid affected by a long-term application of swine manure compost. Environmental Science and Technology, 52, 13270–13278. 
Yan Z J, Chen S, Dari B, Sihi D, Chen Q. 2018. Phosphorus transformation response to soil properties changes induced by manure application in a calcareous soil. Geoderma, 322, 163–171.
Yang X Y, Chen X W, Yang X T. 2019. Effect of organic matter on phosphorus adsorption and desorption in a black soil from Northeast China. Soil and Tillage Research, 187, 85–91.
Zhang H Z, Shi L L, Lu H B, Shao Y H, Liu S R, Fu S L. 2020. Drought promotes soil phosphorus transformation and reduces phosphorus bioavailability in a temperate forest. Science of the Total Environment, 732, 139295.
Zhu J, Li M, Whelan M. 2018. Phosphorus activators contribute to legacy phosphorus availability in agricultural soils: A review. Science of the Total Environment, 612, 522–537.

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