|
Special Issue:
园艺-分子生物合辑Horticulture — Genetics · Breeding
|
|
|
|
| Preharvest application of melatonin induces anthocyanin accumulation and related gene upregulation in red pear (Pyrus ussuriensis) |
SUN Hui-li1*, WANG Xin-yue1*, SHANG Ye1, WANG Xiao-qian1, 2, DU Guo-dong1, 2, LÜ De-guo1, 2 |
1 College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China
2 Key Laboratory of Fruit Quality Development and Regulation of Liaoning Province, Shenyang Agricultural University, Shenyang 110866, P.R.China |
|
|
|
|
摘要
花青素是决定梨果皮颜色的重要成分。研究发现褪黑素可以影响花青素代谢，但褪黑素采前处理对果实着色的作用尚不清楚。本试验以‘南红梨’为材料，研究了50、200 μM褪黑素采前喷施梨果实，对果皮着色、酚类物质含量及相关基因表达的影响。结果表明，褪黑素采前喷施可以显著影响梨果实着色，提高果皮中花青素和黄酮醇的含量，降低羟基肉桂酸和黄烷醇的含量，同时增加多数花青素合成基因和相关转录因子的相对表达量。此外，外源褪黑素处理促进了褪黑素合成相关基因的表达，从而增加果皮中内源褪黑素的含量。试验结果为探索褪黑素调控果实花青素代谢提供了新的思路，并有助于外源褪黑素在农业上的应用。
Abstract Anthocyanins are important components in the peel of red pears and contribute to the appearance of the fruit. Melatonin application is known to affect anthocyanin biosynthesis, but the effect of preharvest melatonin application on fruit coloration remains largely unknown. The objective of this study was to determine the effects of preharvest melatonin application on pigmentation, phenolic compounds, and the expression of related genes in Nanhong pear (Pyrus ussuriensis). The applications were performed during the pre-color-change period by spraying 50 or 200 μmol L–1 of melatonin on fruits. We found that treatment with melatonin had a significant effect on color development. The concentrations of anthocyanins and favonols were enhanced by melatonin treatment, whereas hydroxycinnamate and favanol concentrations were reduced. Quantitative real-time PCR analyses indicated that the transcription levels for most anthocyanin biosynthetic genes and anthocyanin-related transcription factors were induced by melatonin. Melatonin application also stimulated the expression of melatonin biosynthesis-related genes and consequently caused an increase in endogenous melatonin concentration. These results provide insights into melatonin-induced fruit coloration and will facilitate the application of exogenous melatonin in agriculture.
|
|
Received: 24 March 2020
Accepted:
|
| Fund: This research was supported by the National Natural Science Foundation of China (31701865), the earmarked fund for the China Agriculture Research System (CARS-28), and the Liaoning Provincial Natural Science Foundation of China (2019-MS-276). |
|
Corresponding Authors:
Correspondence WANG Xiao-qian, Tel: +86-24-88487219, E-mail: wangxq873@syau.edu.cn; DU Guo-dong, Tel: +86-24-88487219, E-mail: guodongdu@syau.edu.cn
|
| About author: * These authors contributed equally to this study. |
Cite this article:
SUN Hui-li, WANG Xin-yue, SHANG Ye, WANG Xiao-qian, DU Guo-dong, LÜ De-guo.
2021.
Preharvest application of melatonin induces anthocyanin accumulation and related gene upregulation in red pear (Pyrus ussuriensis). Journal of Integrative Agriculture, 20(8): 2126-2137.
|
Ai Y, Zhu Z. 2018. Melatonin antagonizes jasmonate-triggered anthocyanin biosynthesis in Arabidopsis thaliana. Journal of Agricultural and Food Chemistry, 66, 5392–5400.
Arnao M B, Hernández-Ruiz J. 2018. Melatonin in its relationship to plant hormones. Annals of Botany, 121, 195–207.
Arnao M B, Hernández-Ruiz J. 2019a. Melatonin: A new plant hormone and/or a plant master regulator? Trends in Plant Science, 24, 38–48.
Arnao M B, Hernández-Ruiz J. 2019b. Melatonin and reactive oxygen and nitrogen species: A model for the plant redox network. Melatonin Research, 2, 152–168.
Arnao M B, Hernández-Ruiz J. 2019c. Role of melatonin to enhance phytoremediation capacity. Applied Sciences, 9, 5293.
Arnao M B, Hernández-Ruiz J. 2020a. Is phytomelatonin a new plant hormone? Agronomy, 10, 95.
Arnao M B, Hernández-Ruiz J. 2020b. Melatonin in flowering, fruit set and fruit ripening. Plant Reproduction, doi: 10.1007/s00497-020-00388-8.
Bai S L, Sun Y W, Qian M J, Yang F X, Ni J B, Tao R Y, Li L, Shu Q, Zhang D, Teng Y W. 2017. Transcriptome analysis of bagging-treated red Chinese sand pear peels reveals light-responsive pathway functions in anthoccyanin accumulation. Scientific Reports, 7, 1–15.
Chen J X, Mao L C, Mi H B, Lu W J, Ying T J, Luo J S. 2016. Involvement of abscisic acid in postharvest water-deficit stress associated with the accumulation of anthocyanins in strawberry fruit. Postharvest Biology and Technology, 111, 99–105.
Chen L, Tian J, Wang S F, Song T T, Zhang J, Yao Y C. 2019. Application of melatonin promotes anthocyanin accumulation in crabapple leaves. Plant Physiology and Biochemistry, 142, 332–341.
Cui T, Nakamura K, Ma L, Li J Z, Kayahara H. 2005. Analyses of arbutin and chlorogenic acid, the major phenolic constituents in oriental pear. Journal of Agricultural and Food Chemistry, 53, 3882–3887.
Debnath B, Hussain M, Li M, Lu X C, Sun Y T, Qiu D L. 2018. Exogenous melatonin improves fruit quality features, health promoting antioxidant compounds and yield traits in tomato fruits under acid rain stress. Molecules, 23, 1868.
Debnath B, Islam W, Li M, Sun Y T, Lu X C, Mitra S, Hussain M, Liu S, Qiu D L. 2019. Melatonin mediates enhancement of stress tolerance in plants. International Journal of Molecular Sciences, 20, 1040.
Feng S Q, Wang Y L, Yang S, Xu Y T, Chen X S. 2010. Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10. Planta, 232, 245–255.
Flores G, Ruiz del Castillo M L. 2016. Accumulation of anthocyanins and flavonols in black currants (Ribes nigrum L.) by pre-harvest methyl jasmonate treatments. Journal of the Science of Food and Agriculture, 96, 4026–4031.
He J G, Cheng Y D, Guan J F, Ge W Y, Zhao Z. 2017. Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development. Journal of Integrative Agriculture, 16, 471–477.
Henry-Kirk R A, McGhie T K, Andre C M, Hellens R P, Allan A C. 2012. Transcriptional analysis of apple fruit proanthocyanidin biosynthesis. Journal of Experimental Botany, 63, 5437–5450.
Huang C H, Yu B, Teng Y W, Su J, Shu Q, Ceng J Q, Zeng L Q. 2009. Effects of fruit bagging on coloring and related physiology, and qualities of red Chinese sand pears during fruit maturation. Scientia Horticulturae, 121, 149–158.
Li D, Zhang X C, Xu Y Q, Li L, Aghdam M S, Luo Z S. 2019. Effect of exogenous sucrose on anthocyanin synthesis in postharvest strawberry fruit. Food Chemistry, 289, 112–120.
Liu B Y, Wang L, Wang S M, Li W J, Liu D, Guo X F, Qu B H. 2019. Transcriptomic analysis of bagging-treated ‘Pingguo’ pear shows that MYB4-like1, MYB4-like2, MYB1R1 and WDR involved in anthocyanin biosynthesis are up-regulated in fruit peels in response to light. Scientia Horticulturae, 244, 428–434.
Liu C H, Zheng H H, Sheng K L, Liu W, Zheng L. 2018. Effects of melatonin treatment on the postharvest quality of strawberry fruit. Postharvest Biology and Technology, 139, 47–55.
Liu Y L, Che F, Wang L X, Meng R, Zhang X J, Zhao Z Y. 2013. Fruit coloration and anthocyanin biosynthesis after bag removal in non-red and red apples (Malus domestica Borkh.). Molecules, 18, 1549–1563.
Meng J F, Yu Y, Shi T C, Fu Y S, Zhao T, Zhang Z W. 2019. Melatonin treatment of pre-veraison grape berries modifies phenolic components and antioxidant activity of grapes and wine. Food Science and Technology, 39, 35–42.
Moustafa-Farag M, Almoneafy A, Mahmoud A, Elkelish A, Arnao M B, Li L F, Ai S Y. 2020. Melatonin and its protective role against biotic stress impacts on plants. Biomolecules, 10, 54.
Mukherjee S. 2019. Recent advancements in the mechanism of nitric oxide signaling associated with hydrogen sulfide and melatonin crosstalk during ethylene-induced fruit ripening in plants. Nitric Oxide, 82, 25–34.
Ni J B, Bai S L, Zhao Y, Qian M J, Tao R Y, Yin L, Gao L, Teng Y W. 2019. Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in ‘Red Zaosu’ pear fruits by interacting with MYB114. Plant Molecular Biology, 99, 67–78.
Portu J, Santamaria P, Lopez-Alfaro L, López R, Garde-Cerdán T. 2015. Methyl jasmonate foliar application to tempranillo vineyard improved grape and wine phenolic content. Journal of Agricultural and Food Chemistry, 63, 2328–2337.
Pothinuch P, Tongchitpakdee S. 2011. Melatonin contents in mulberry (Morus spp.) leaves: Effects of sample preparation, cultivar, leaf age and tea processing. Food Chemistry, 128, 415–419.
Qian M J, Yu B, Li X, Sun Y W, Zhang D, Teng Y W. 2014. Isolation and expression analysis of anthocyanin biosynthesis genes from the red chinese sand pear, Pyrus pyrifolia Nakai cv. Mantianhong, in response to methyl jasmonate treatment and UV-B/VIS conditions. Plant Molecular Biology Reporter, 32, 428–437.
Ruiz-García Y, Romero-Cascales I, Belén Bautista-Ortín A, Gil-Muñoz R, Martínez-Cutillas A, Gómez-Plaza E. 2013. Increasing bioactive phenolic compounds in grapes: Response of six monastrell grape clones to benzothiadiazole and methyl jasmonate treatments. American Journal of Enology and Viticulture, 64, 459–465.
Schaart J G, Dubos C, Fuente I R D L, Houwelingen A M M L, Vos R C H, Jonker H H, Xu W J, Routaboul J M, Lepiniec L, Bovy A G H. 2013. Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria ananassa) fruits. New Phytologist, 197, 454–467.
Sharif R, Xie C, Zhang H, Arnao M B, Ali M, Ali Q, Muhammad I, Shalmani A, Nawaz M A, Chen P, Li Y H. 2018. Melatonin and its effects on plant systems. Molecules, 23, 2352.
Sun Q Q, Zhang N, Wang J F, Cao Y Y, Li X S, Zhang H J, Zhang L, Tan D X, Guo Y D. 2016. A label-free differential proteomics analysis reveals the effect of melatonin on promoting fruit ripening and anthocyanin accumulation upon postharvest in tomato. Journal of Pineal Research, 61, 138–153.
Sun Y L, Liu K J, Xi B, Dai H J. 2019. Study on the regulation of anthocyanin biosynthesis by exogenous abscisic acid in grapevine. Scientia Horticulturae, 250, 294–301.
Wang S Y, Shi X C, Wang R, Wang H L, Liu F, Laborda P. 2020. Melatonin in fruit production and postharvest preservation: A review. Food Chemistry, 320, 126642.
Wang X, Liu S, Liu C, Liu Y, Lu X, Du G, Lyu D. 2020. Biochemical characterization and expression analysis of lignification in two pear (Pyrus ussuriensis Maxim.) varieties with contrasting stone cell content. Protoplasma, 257, 261–274.
Wang X, Wei Z, Ma F. 2015. The effects of fruit bagging on levels of phenolic compounds and expression by anthocyanin biosynthetic and regulatory genes in red-fleshed apples. Process Biochemistry, 50, 1774–1782.
Xie Q, Yan F, Hu Z, Wei S, Lai J, Chen G. 2019. Accumulation of anthocyanin and its associated gene expression in purple tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) sprouts when exposed to light, dark, sugar and methyl jasmonate. Journal of Agricultural and Food Chemistry, 67, 856–866.
Xu L L, Yue Q Y, Bian F, Zhai H, Yao Y X. 2017. Melatonin enhances phenolics accumulation partially via ethylene signaling and resulted in high antioxidant capacity in grape berries. Frontiers in Plant Science, 8, 1426.
Xu T, Chen Y, Kang H. 2019. Melatonin is a potential target for improving post-harvest preservation of fruits and vegetables. Frontiers in Plant Science, 10, 1388.
Yao G F, Ming M L, Allan A C, Gu X, Li L, Wu X, Wang R Z, Chang Y J, Qi K J, Zhang S L, Wu J. 2017. Map-based cloning of the pear gene MYB114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis. The Plant Journal, 92, 437–451.
Yan Y Y, Shi Q H, Gong B. 2020. Review of melatonin in horticultural crops. Melatonin, doi: 10.5772/intechopen.90935.
Zhai R, Liu J L, Liu F X, Zhao Y X, Liu L L, Fang C, Wang H B, Li X Y, Wang Z G, Ma F W, Xu L F. 2018. Melatonin limited ethylene production, softening and reduced physiology disorder in pear (Pyrus communis L.) fruit during senescence. Postharvest Biology and Technology, 193, 38–46.
Zhang D, Qian M J, Yu B, Teng Y W. 2013. Effect of fruit maturity on UV-B-induced post-harvest anthocyanin accumulation in red Chinese sand pear. Acta Physiologiae Plantarum, 35, 2857–2866.
Zhang N, Sun Q Q, Li H F, Li X S, Cao Y Y, Zhang H J, Li S T, Zhang L, Qi Y, Ren S X, Zhao, B, Guo Y D. 2016. Melatonin improved anthocyanin accumulation by regulating gene expressions and resulted in high reactive oxygen species scavenging capacity in cabbage. Frontiers in Plant Science, 7, 197.
Zhao Y, Tan D X, Lei Q, Chen H, Wang L, Li Q T, Gao Y A, Kong J. 2012. Melatonin and its potential biological functions in the fruits of sweet cherry. Journal of Pineal Research, 55, 79–88.
Zhu Y F, Su J, Yao G F, Liu H N, Gu C, Qin M F, Bai B, Cai S S, Wang G M, Wang R Z, Shu Q, Wu J. 2018. Different light-response patterns of coloration and related gene expression in red pears (Pyrus L.). Scientia Horticulturae, 229, 240–251.
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
