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Special Issue:
水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources
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| A rice geranylgeranyl reductase is essential for chloroplast development |
| LIU Xi, YI Xin, YANG Yan-rong, HUANG Qian-qian |
| Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huai’an 223300, P.R.China |
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摘要
叶绿体对植物光合作用和生长是必要的。许多基因被鉴定参与调控植物叶绿体发育。然而，在分子水平上这些基因如何调控叶绿体生物合成是未知的。在本研究中，我们分离了一个色素缺乏的突变体ygl2。YGL2编码一个牻牛儿基牻牛儿基还原酶。YGL2的第三外显子有一个碱基突变（T1361G），导致编码产物发生错义突变（L454R）。透射电镜显示，突变体ygl2的叶绿体发育受损。在突变体ygl2中，质体编码基因的表达水平发生显著变化。此外，通过酵母双杂交分析我们发现YGL2与RNA编辑因子MORF8互作。
Abstract Chloroplasts are essential for plant photosynthesis and growth. Many genes have been identified that regulate plant chloroplast development. However, it is not known at a molecular level how these genes regulate chloroplast biogenesis. In this study, we isolated a mutant ygl2 (yellow-green leaf2) that exhibited a pigment-defective phenotype. YGL2 encodes a geranylgeranyl reductase, and in mutant ygl2, there was a single base change (T1361G) located in the third exon of YGL2 that resulted in a missense mutation (L454R) in the encoded product. Transmission electron microscopy revealed that chloroplast development was impaired in the ygl2 mutant. The expression levels of plastid-encoded genes were significantly altered in the ygl2 mutant. Furthermore, in a yeast two-hybrid assay, we found that YGL2 interacted with the RNA editing factor MORF8.
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Received: 07 February 2020
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31900388), the Natural Science Foundation of Jiangsu Province, China (BK20191055) and the Natural Science Foundation of Colleges of Jiangsu Province, China (19KJB180009).
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Corresponding Authors:
Correspondence LIU Xi, Tel: +86-517-83525991, E-mail:1240623244@qq.com
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Cite this article:
LIU Xi, YI Xin, YANG Yan-rong, HUANG Qian-qian.
2021.
A rice geranylgeranyl reductase is essential for chloroplast development. Journal of Integrative Agriculture, 20(10): 2592-2600.
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