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Journal of Integrative Agriculture  2021, Vol. 20 Issue (3): 821-828    DOI: 10.1016/S2095-3119(19)62863-7
Section 4: Integrated pest management Advanced Online Publication | Current Issue | Archive | Adv Search |
Analysis of phototactic responses in Spodoptera frugiperda using Helicoverpa armigera as control
LIU Ying-jie1, 2, ZHANG Dan-dan2, YANG Li-yu1, DONG Yong-hao1, LIANG Ge-mei2, Philip DONKERSLEY3, REN Guang-wei1, XU Peng-jun1, WU Kong-ming2 
1 Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, P.R.China
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK
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摘要  

诱虫灯在现代农业的害虫预测预报和防治工作中得到广泛的应用。草地贪夜蛾于2018年底经云南迁飞入侵中国,严重威胁我国农作物的安全生产。为明确诱虫灯对草地贪夜蛾成虫的诱集效率,我们首先通过转录组测序确定其视蛋白基因的种类和数量。聚类分析表明,与其它夜蛾科昆虫类似,草地贪夜蛾表达4个视蛋白基因。但是草地贪夜蛾视蛋白基因的表达量显著低于棉铃虫中相对应的基因。趋光行为研究表明,棉铃虫和草地贪夜蛾成虫均具有显著正趋光性,其中草地贪夜蛾雌、雄成虫飞向光源的速度均显著高于棉铃虫,这可能是由于草地贪夜蛾飞行速度高于棉铃虫所致。然而,诱虫灯结果表明草地贪夜蛾的上灯率显著低于棉铃虫,这与视蛋白基因在两种昆虫中的表达水平一致。综上所述,本研究表明草地贪夜蛾成虫具有趋光性,诱虫灯可用于该虫的预测预报及防治工作,但诱集效率低于棉铃虫。




Abstract  
Light traps are widely utilized to monitor and manage insect pest populations.  In late 2018, the fall armyworm (FAW), Spodoptera frugiperda, invaded China through Yunnan Province representing a huge threat to grain production.  To estimate the efficiency of light traps on FAW moths, we first identified the opsin genes from FAW by using the transcriptome.  Phylogenetic analysis indicated that the four opsins of FAW were clustered with those of other Noctuidae species.  The expressed levels of opsins in S. frugiperda were lower than in Helicoverpa armigera, suggesting a different phototactic response between the two species.  Then, we determined the phototactic behavior of FAW using H. armigera as a control, which is widely monitored and managed using light traps in China.  Our results indicated that the two moths species showed significantly different phototactic behavior and both female and male FAW displayed faster flight-to-light speed than H. armigera.  This may be due to a faster flight capacity in FAW compared to H. armigera.  However, the capture rate of both female and male of S. frugiperda was significantly lower than that of H. armigera, which was consistent with the expression levels of opsins.  These results support the positive phototaxis of S. frugiperda moths and suggest light traps could be used for monitoring and managing the pests, but with a lower efficiency than H. armigera.
Keywords:  Spodoptera frugiperda        Helicoverpa armigera        light performance        opsin genes        light trap  
Received: 12 September 2019   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2017YFB0403905), the Central Public-interest Scientific Institution Basal Research Fund, China (Y2019YJ06), and the Agricultural Science and Technology Innovation Program of China (ASTIP-TRIC04).
Corresponding Authors:  Correspondence XU Peng-jun, E-mail: xupengjun@163.com; REN Guang-wei, E-mail: renguangwei@caas.cn   

Cite this article: 

LIU Ying-jie, ZHANG Dan-dan, YANG Li-yu, DONG Yong-hao, LIANG Ge-mei, Philip DONKERSLEY, REN Guang-wei, XU Peng-jun, WU Kong-ming . 2021. Analysis of phototactic responses in Spodoptera frugiperda using Helicoverpa armigera as control. Journal of Integrative Agriculture, 20(3): 821-828.

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