|
|
|
|
|
| Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana |
ZHANG Ya-bin1*, TANG Wei1, 2*, WANG Li-huan1, HU Ya-wen1, LIU Xian-wen2, LIU Yong-sheng1, 2 |
1 Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China
2 School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, P.R.China |
|
|
|
|
Abstract Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses, such as drought, heat, cold, water logging and phytopathogens. Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement. Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog (AcMYB3R) whose expression was induced by drought, salinity and cold stress. In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE. The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress. The expressions of stress-responsive genes such as RD29A, RD29B, COR15A and RD22 were prominently up-regulated by ectopic expression of AcMYB3R. Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.
|
|
Received: 29 January 2018
Accepted:
|
| Fund: This work was financially supported by the National Natural Science Foundation of China (31471157 and 31700266). |
|
Corresponding Authors:
Correspondence LIU Yong-sheng, Mobile: +86-18709832886, E-mail: liuyongsheng1122@hfut.edu.cn; TANG Wei, Mobile: +86-13339017320, E-mail: tangweitw@aliyun.com.cn
|
| About author:
ZHANG Ya-bin, Mobile: +86-18227630935, E-mail: kiwifruitactinidia@163.com;
* These authors contributed equally to this study. |
Cite this article:
ZHANG Ya-bin, TANG Wei, WANG Li-huan, HU Ya-wen, LIU Xian-wen, LIU Yong-sheng.
2019.
Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana. Journal of Integrative Agriculture, 18(2): 417-427.
|
| Allan A C, Hellens R P, Laing W A. 2008. MYB transcription factors that colour our fruit. Trends in Plant Science, 13, 99–102.
Cai H S, Tian S, Dong H S, Guo C H. 2015. Pleiotropic effects of TaMYB3R1 on plant development and response to osmotic stress in transgenic Arabidopsis. Gene, 558, 227–234.
Cai H S, Tian S, Liu C L, Dong H S. 2011. Identification of a MYB3R gene involved in drought, salt and cold stress in wheat (Triticum aestivum L.). Gene, 485, 146–152.
Dai X Y, Xu Y Y, Ma Q B, Xu W Y, Wang T, Xue Y B, Chong K. 2007. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiology, 143, 1739–1751.
Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. 2010. MYB transcription factors in Arabidopsis. Trends in Plant Science, 15, 573–581.
Feng G Q, Burleigh J G, Braun E L, Mei W B, Barbazuk W B. 2017. Evolution of the 3R-MYB gene family in plants. Genome Biology and Evolution, 9, 1013–1029.
Gao A L, Wu Q Y, Zhang Y, Miao Y C, Song C P. 2014. Arabidopsis calcium-dependent protein kinase CPK28 is potentially involved in the response to osmotic stress. Chinese Science Bulletin, 59, 1113–1122.
Gietz D, Stjean A, Woods R A, Schiestl R H. 1992. Improved method for high-efficiency transformation of intact yeast-cells. Nucleic Acids Research, 20, 1425–1425.
Haga N, Kato K, Murase M, Araki S, Kubo M, Demura T, Suzuki K, Muller I, Voss U, Jurgens G, Ito M. 2007. R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana. Development, 134, 1101–1110.
Haga N, Kobayashi K, Suzuki T, Maeo K, Kubo M, Ohtani M, Mitsuda N, Demura T, Nakamura K, Jurgens G, Ito M. 2011. Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis. Plant Physiology, 157, 706–717.
Hu J T, Chen G P, Yin W C, Cui B L, Yu X H, Lu Y, Hu Z L. 2017. Silencing of SlHB2 improves drought, salt stress tolerance, and induces stress-related gene expression in tomato. Journal of Plant Growth Regulation, 36, 578–589.
Huang S X, Ding J, Deng D J, Tang W, Sun H H, Liu D Y, Zhang L, Niu X L, Zhang X, Meng M, Yu J D, Liu J, Han Y, Shi W, Zhang D F, Cao S Q, Wei Z J, Cui Y L, Xia Y H, Zeng H P, et al. 2013. Draft genome of the kiwifruit Actinidia chinensis. Nature Communications, 4, 2640.
Ito M, Araki S, Matsunaga S, Itoh T, Nishihama R, Machida Y, Doonan J H, Watanabe A. 2001. G2/M-phase–specific transcription during the plant cell cycle is mediated by c-Myb–like transcription factors. Plant Cell, 13, 2159–2159.
Jefferson R A, Kavanagh T A, Bevan M W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher-plants. EMBO Journal, 6, 3901–3907.
Jin H L, Martin C. 1999. Multifunctionality and diversity within the plant MYB-gene family. Plant Molecular Biology, 41, 577–585.
Li T, Sun J K, Bi Y P, Peng Z Y. 2016. Overexpression of an MYB-related gene FvMYB1 from fraxinus velutina increases tolerance to salt stress in transgenic tobacco. Journal of Plant Growth Regulation, 35, 632–645.
Li Y X, Deng H, Miao M, Li H R, Huang S X, Wang S H, Liu Y S. 2016. Tomato MBD5, a methyl CpG binding domain protein, physically interacting with UV-damaged DNA binding protein-1, functions in multiple processes. New Phytologist, 210, 208–226.
Liu F F, Guo X H, Yao Y C, Tang W, Zhang W, Cao S Q, Han Y, Liu Y S. 2016. Cloning and function characterization of two dehydroascorbate reductases from kiwifruit (Actinidia chinensis L.). Plant Molecular Biology Reporter, 34, 815–826.
Liu J K, Li H J, Miao M, Tang X F, Giovannoni J, Xiao F M, Liu Y S. 2012. The tomato UV-damaged DNA-binding protein-1 (DDB1) is implicated in pathogenesis-related (PR) gene expression and resistance to Agrobacterium tumefaciens. Molecular Plant Pathology, 13, 123–134.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 25, 402–408.
Ma Q B, Dai X Y, Xu Y Y, Guo J, Liu Y J, Chen N, Xiao J, Zhang D J, Xu Z H, Zhang X S, Chong K. 2009. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes. Plant Physiology, 150, 244–256.
Mittler R, Blumwald E. 2010. Genetic engineering for modern agriculture: Challenges and perspectives. Annual Review of Plant Biology, 61, 443–462.
Rosinski J A, Atchley W R. 1998. Molecular evolution of the Myb family of transcription factors: Evidence for polyphyletic origin. Journal of Molecular Evolution, 46, 74–83.
Song L, Huang S S C, Wise A, Castanon R, Nery J R, Chen H M, Watanabe M, Thomas J, Bar-Joseph Z, Ecker J R. 2016. A transcription factor hierarchy defines an environmental stress response network. Science, 354, aag1550.
Tang W, Sun J Q, Liu J, Liu F F, Yan J, Gou X J, Lu B R, Liu Y S. 2014. RNAi-directed downregulation of betaine aldehyde dehydrogenase 1 (OsBADH1) results in decreased stress tolerance and increased oxidative markers without affecting glycine betaine biosynthesis in rice (Oryza sativa). Plant Molecular Biology, 86, 443–454.
Tang W, Zheng Y, Dong J, Yu J, Yue J Y, Liu F F, Guo X H, Huang S X, Wisniewski M, Sun J Q, Niu X L, Ding J, Liu J, Fei Z J, Liu Y S. 2016. Comprehensive transcriptome profiling reveals long noncoding RNA expression and alternative splicing regulation during fruit development and ripening in kiwifruit (Actinidia chinensis). Frontiers in Plant Science, 7, 335.
Yamaguchi-Shinozaki K, Shinozaki K. 2001. Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Rice Biotechnology: Improving Yield, Stress Tolerance and Grain Quality, 236, 176–189.
Yamaguchi-Shinozaki K, Shinozaki K. 2006. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology, 57, 781–803.
Yang Y N, Li R G, Qi M. 2000. In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves. The Plant Journal, 22, 543–551.
Yue J Y, Liu J, Ban R J, Tang W, Deng L, Fei Z J, Liu Y S. 2015. Kiwifruit information resource (KIR): A comparative platform for kiwifruit genomics. Database - The Journal of Biological Databases and Curation, 2015, bav113.
Zhang X R, Henriques R, Lin S S, Niu Q W, Chua N H. 2006. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nature Protocols, 1, 641–646.
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
