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Journal of Integrative Agriculture  2018, Vol. 17 Issue (12): 2601-2611    DOI: 10.1016/S2095-3119(18)61995-1
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Analysis of three types of resistance gene analogs in PmU region from Triticum urartu
ZHANG Lei1, ZHENG Xing-wei2, QIAO Lin-yi3, QIAO Ling2, ZHAO Jia-jia2, WANG Jian-ming1, ZHENG Jun2
1 College of Agriculture, Shanxi Agricultural University, Taigu 030801, P.R.China
2 Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen 041000, P.R.China
3 Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, P.R.China
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Abstract  Resistance gene analog (RGA) screening of mapped disease-resistant genes not only helps to clone these genes but also helps to develop efficient molecular markers for resistance breeding. The present study focused on the PmU region located on chromosome 7AuL of Triticum urartu, and recently, a nucleotide binding site (NBS)-encoding gene, Pm60, was cloned from the same chromosome arm. In this research, NBS, protein kinase (PK), and ATP-binding cassette (ABC), the three disease resistance-related gene families, were analyzed within PmU region by using informatics tools, and an expression experiment was conducted to verify their functions in vivo. Comparative genomic analysis revealed that 126 RGAs were included on chromosome 7AuL, and 30 of the RGAs as well as Pm60 were found in the PmU region. Transcriptome database analysis of T. urartu revealed 14 PmU-RGAs with expression data, and three PmU-NBSs exhibited significant changes in expression after inoculation with Blumeria graminis f. sp. tritici (Bgt); TRIUR3_14879 was up-regulated, while TRIUR3_00450 and TRIUR3_06270 were down-regulated. Cluster analysis showed that these three PmU-NBSs were clustered far from the cloned wheat resistance genes. Then, qRT-PCR was performed to investigate the expression of 14 PmU-RGAs and Pm60 after inoculation with Bgt race E09; the results showed that Pm60 was specifically expressed in UR206 which carrying PmU, but not in susceptible UR203; while TRIUR3_14879 was significantly up-regulated and TRIUR3_00450 was downregulated in UR206 after inoculation. These results indicated that PmU is Pm60, and TRIUR3_14879 and TRIUR3_00450 may also be involved in the defense against Bgt.
Keywords:  cluster analysis        expression analysis        nucleotide binding site (NBS)        powdery mildew        protein kinase (PK)  
Received: 16 November 2017   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31601307), the Key Scientific and Technological Innovation Platform of the Main Crop Germplasm Innovation and Molecular Breeding in Shanxi Province, China (201605D151002) and the Youth Foundation of Institute of Crop Science, Shanxi Academy of Agricultural Sciences (ZZQ1701).
Corresponding Authors:  Correspondence ZHENG Jun, Tel: +86-357-2882445,E-mail:; WANG Jian-ming, Tel: +86-354-6289739, E-mail:   
About author:  ZHANG Lei, Tel: +86-357-2882445, E-mail:;

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ZHANG Lei, ZHENG Xing-wei, QIAO Lin-yi, QIAO Ling, ZHAO Jia-jia, WANG Jian-ming, ZHENG Jun. 2018. Analysis of three types of resistance gene analogs in PmU region from Triticum urartu. Journal of Integrative Agriculture, 17(12): 2601-2611.

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