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Journal of Integrative Agriculture  2018, Vol. 17 Issue (04): 857-866    DOI: 10.1016/S2095-3119(17)61760-X
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Physiological mechanisms of resistance to cold stress associated with 10 elite apple rootstocks
WANG Yan-xiu1, HU Ya1, CHEN Bai-hong1, ZHU Yan-fang1, Mohammed Mujitaba Dawuda1, 2, Sofkova Svetla
1 College of Horticulture, Gansu Agricultural University, Lanzhou 730070, P.R.China
2 Deparment of Horticulture, Faculty of Agriculture, University for Development Studies, Tamale P.O. BoxTL 1882, Ghanas
3 Institute of Agriculture and Environment, Massey University, Palmerston North 4442, New Zealand
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Abstract  A study was conducted in attempting to identify the cold-resistant apple rootstocks and to establish a comprehensive evaluation system.  In this study, 10 elite apple dwarfing rootstocks (GM256, JM7, M26, M7, SC1, SH1, SH38, SH6, M9, and T337) were employed for the experiment and the following parameters were investigated under different low temperature stress conditions (0, –15, –20, –25, –30, and –35°C): the changes of the relative electrical conductivity (REC), anthocyanin content, protein content, soluble sugar content, soluble starch content, proline content, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and peroxidase (POD) activity of the dormant branches.  The inflection temperature that could represent the plant tissue semi-lethal temperature (LT50) was obtained by the measurements of REC.  The LT50 was used to evaluate eight other indices.  The results showed that there was no significant correlation between LT50 and POD activity as well as between the soluble sugar, protein and proline contents at 0 and –15°C.  Soluble starch content at 0 and –15°C and anthocyanin content at –15–(–30)°C were significantly but negatively correlated to the LT50 and the MDA content at 0–(–20)°C was significantly positively correlated to the LT50.  Statistical analysis based on principal component analysis and LT50 showed that cold resistant apple rootstocks in the decreasing order from high to low as GM256, SH6, SH38, SH1, SC1, M26, M7, JM7, T337, and M9.
Keywords:  cold resistance        LT50        principal component analysis        apple dwarfing rootstock  
Received: 06 March 2017   Accepted:

This work was supported by the Fundamental Research Funds for the Universities of Gansu Province, China (035-041051) and the Natural Science Foundation of Gansu Province, China (145RJZA167).

Corresponding Authors:  WANG Yan-xiu, Mobile: +86-13919489161, E-mail:   

Cite this article: 

WANG Yan-xiu, HU Ya, CHEN Bai-hong, ZHU Yan-fang, Mohammed Mujitaba Dawuda, Sofkova Svetla. 2018. Physiological mechanisms of resistance to cold stress associated with 10 elite apple rootstocks. Journal of Integrative Agriculture, 17(04): 857-866.

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