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| Charactering protein fraction concentrations as influenced by nitrogen application in low-glutelin rice cultivars |
| LI Gang-hua, CHEN Yi-lu, DING Yan-feng, GENG Chun-miao, LI Quan, LIU Zheng-hui, WANG Shao-hua, TANG She |
| Jiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.China |
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摘要 To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars (W1240, W1721, W025) and an ordinary rice cultivar (H9405) with five N treatments were carried out to determine the effects of N application rate and genotype on protein fractions contents and Glutelin/Prolamin ratio (Glu/Pro). The difference of protein fraction concentrations affected by N application rate existed in genotypes. Ordinary rice cultivar had a larger increase in glutlein concentration affected by N application rate than low-glutelin rice cultivars did. Glutelin in H9405 had a increase of 30.6 and 41.0% under the N4 treatment (360 kg N ha–1) when compared with N0 treatment (no fertilizer N) in 2010 and 2011 respectively, while all the low-glutelin rice cultivars showed relatively smaller increases for two years. Variance analysis showed no significant effect of N application rate on glutelin in W1240 and W025 while the effects on albumin, globulin and prolamin were significant in low-glutelin rice. What’s more, N treatment had no significant influence on Glu/Pro ratios in low-glutelin rice cultivars while a significant increase in Glu/Pro ratio was observed in ordinary rice cultivar. So low-gultelin rice cultivars showed a different pattern from ordinary rice cultivars when influenced by N application rate.
Abstract To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars (W1240, W1721, W025) and an ordinary rice cultivar (H9405) with five N treatments were carried out to determine the effects of N application rate and genotype on protein fractions contents and Glutelin/Prolamin ratio (Glu/Pro). The difference of protein fraction concentrations affected by N application rate existed in genotypes. Ordinary rice cultivar had a larger increase in glutlein concentration affected by N application rate than low-glutelin rice cultivars did. Glutelin in H9405 had a increase of 30.6 and 41.0% under the N4 treatment (360 kg N ha–1) when compared with N0 treatment (no fertilizer N) in 2010 and 2011 respectively, while all the low-glutelin rice cultivars showed relatively smaller increases for two years. Variance analysis showed no significant effect of N application rate on glutelin in W1240 and W025 while the effects on albumin, globulin and prolamin were significant in low-glutelin rice. What’s more, N treatment had no significant influence on Glu/Pro ratios in low-glutelin rice cultivars while a significant increase in Glu/Pro ratio was observed in ordinary rice cultivar. So low-gultelin rice cultivars showed a different pattern from ordinary rice cultivars when influenced by N application rate.
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Received: 31 March 2015
Accepted:
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| Fund: Funding was provided by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B14, 2012BAD20B05, 2012BAD04B08). |
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Corresponding Authors:
DING Yan-feng, Tel/Fax: +86-25-84396475,E-mail: dingyf@njau.edu.cn
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| About author: LI Gang-hua, Tel/Fax: +86-25-84396475, E-mail: lgh@njau.edu.cn; |
Cite this article:
LI Gang-hua, CHEN Yi-lu, DING Yan-feng, GENG Chun-miao, LI Quan, LIU Zheng-hui, WANG Shao-hua, TANG She.
2016.
Charactering protein fraction concentrations as influenced by nitrogen application in low-glutelin rice cultivars. Journal of Integrative Agriculture, 15(3): 537-544.
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| Ashida K, Araki E, Maruyama-Funatsuki W, Fujimoto H, IkegamiM. 2013. Temperature during grain ripening affects the ratioof type-II/type-I protein body and starch pasting propertiesof rice (Oryza sativa L.). Journal of Cereal Science, 57,153-159Baxter G, Zhao J, Blanchard B. 2006. How do endospermproteins affect the pasting and texture of rice? - A studyof the physiochemical properties of rice using a modelsystem. In: IUFost 13th World Congress of Food Sciencesand Technology. Nantes, France. pp. 1105-1116Becthel D B, Juliano B O. 1980. Formation of protein bodiesin the starchy endosperm of rice (Oryza sativa L.). A reinvestigation.Annals of Botany, 45, 503-509Bollag D M, Edelstein S J. 1990. Protein Methods. Wiley-Liss,New York. pp. 50-56Borrell A K, Garside A L, Fukai S, Reid D J. 1999. Grain qualityof flooded rice is affected by season, nitrogen rate, andplant type. Australian Journal of Agricultural Research, 50,1399-1408Cagampang G B, Cruz L J, Espiritu S G, Santiago R G, JulianoB O. 1966. Studies on the extraction and composition ofrice proteins. Cereal Chemistry, 43, 145-155Furukawa S, Mizuma T, Kiyokawa Y, Masumura T, Tanaka K,Wakai Y. 2003. Distribution of storage protein in low-glutelinrice seed determined using a fluorescent antibody. Journalof Bioscience and Bioengineering, 96, 467-473Furukawa S, Mizuma T, Yanagiuchi T, Kiyokawa Y, Wakai Y.2000. Dissolution of protein from rice used for sake brewingin sake mash. Journal of Brewing and Society of Japan,95, 295-303Furukawa S, Itou M, Masumura T, Tanaka K, Kiyokawa Y, WakaiY. 2007. Ultrastructure of low-glutelin rice endosperm. PlantBiotechnology, 24, 227-229.Guo J H, Liu X J, Zhang Y, Shen J L, Han W X, Zhang W F,Christie P, Goulding K, Vitousek P M, Zhang F S. 2010.Significant acidification in major Chinese croplands.Science, 327, 1008-1010Hashizume K, Okuda M, Numata M, Iwashita K. 2007. Bittertastingsake peptides derived from the N-terminus of therice glutelin acidic subunit. Food Science and TechnologyResearch, 13, 270-274Holme D J, Peck H. 1998. Analytical Biochemistry. 3rd ed.Addison Wesley Longman, New York. pp. 388-393Iida S, Sunohara Y, Maeda H, Matsushita K, Nemoto H, IshiiT, Yoshida T, Nakagawa N, Sakai M, Nishio T. 2004. Anew rice cultivar with good eating quality (low amylose)and low glutelin protein, “LGCsoft”. Bulletin of the NationalAgricultural Research Center for Western Region, 3, 57-74Islam N, Inanage S, Chishaki N, Horiguchii T. 1996. Effect of Ntop-dressing on protein content in japonica and indica ricegrains. Cereal Chemistry, 73, 571-573Iwano K, Takahashi K, Ito T, Nakazawa S. 2004. Search foramino acids affecting the taste of Japanese sake. Journalof Brewing and Society of Japan, 99, 659-664Kim J W, Kim B C, Lee J H, Lee D R, Rehman S, Yun S J.2013. Protein content and composition of waxy rice grains.Pakistan Journal of Botany, 45, 151-156Kizaki Y. 1999. Treatment of rice grain and hydrolysis of proteinin milled rice grain during sake making. Journal of Brewingand Society of Japan, 94, 48-50Kusaba M, Miyahara K, Iida S, Fukuoka H, Takano T, SassaH, Nishimura M, Nishio T. 2003. Low glutelin content 1: Adominant mutation that suppresses the glutelin multigenefamily via silencing in rice. The Plant Cell, 15, 1455-1467Leesawatwong M, Jamjod S, Kuo J, Dell B, Rerkasem B. 2005.Nitrogen fertilizer increases seed protein and milling qualityof rice. Cereal Chemistry, 82, 588-593Levey A S, Andreoli S P, DuBose T, Provenzano R, Collins AJ. 2007. Chronic kidney disease: Common, harmful andtreatable - world kidney day 2007. American Journal ofNephrology, 27, 108-112 Luthe D S. 1983. Storage protein accumulation in developingrice (Oryza sativa L.) seeds. Plant Science Letters, 32,147-158Martin M, Fitzgerald M A. 2002. Proteins in rice grains influencecooking properties. Journal of Cereal Science, 36, 285-294Ning H F, Qiao J F, Liu Z H, Lin Z M, Li G H, Wang Q S, Wang SH, Ding Y F. 2010. Distribution of proteins and amino acidsin milled and brown rice as affected by nitrogen fertilizationand genotype. Journal of Cereal Science, 52, 90-95Nishimura M, Kusaba M, Miyahara K, Nishio T, Iida S, Imbe T,Sato H. 2005. New rice cultivars with low levels of easyto-digest protein, “LGC-Katsu” and “LGC-Jun”. BreedingScience, 55, 103-105Ohdaira Y, Takeda H, Sasaki R. 2010. Effects of temperatureon the digestible protein content of grains during ripening inseed-protein mutant rice cultivar LGCsoft. Plant ProductionScience, 13, 132-140Ohta E, Akazawa M, Noda Y, Mandai S, Naito S, Ohta A,Sohara E, Okado T, Rai T, Uchida S, Sasaki S. 2012.Severe hyperparathyroidism in a pre-dialysis chronic kidneydisease patient treated with a very low protein diet. Journalof Bone and Mineral Metabolism, 30, 238-242Peng S, Buresh R J, Huang J, Zhong X, Zou Y, Yang J, WangG, Liu Y, Hu R, Tang Q, Cui K, Zhang F, Dobermann A.2010. Improving nitrogen fertilization in rice by site-specificN management. A review. Agronomy for SustainableDevelopment, 30, 649-656Su N, Wan X Y, Zhai H Q, Wan J M. 2008. Progress andProspect of functional rice researches. Agricultural Sciencesin China, 7, 1-9Tanaka K, Sugimoto T, Ogawa M, Kasai Z. 1980. Isolationand characterization of two types of protein bodies in therice endosperm. Agricultural and Biological Chemistry, 47,1633-1639Yoshizawa K, Ogawa Y. 2004. Rice in brewing. In: ChampagneE T, ed., RICE: Chemistry and Technology. 3rd ed. AACCInternational, Paul, MN, USA. pp. 541-567Zhang W, Bi J, Chen L, Zheng L, Ji S, Xia Y, Xie K, Zhao Z,Wang Y, Liu L, Jiang L, Wan J. 2008. QTL mapping for crudeprotein and protein fraction contents in rice (Oryza sativaL.). Journal of Cereal Science, 48, 539-547 |
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