|
|
|
|
|
| Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) |
| CHEN Tai-yu1, 2, HOU Ji-xiang1, LIN Yong-jun1 |
1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China
2 College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China |
|
|
|
摘要 RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N. cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.
Abstract RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N. cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.
|
|
Received: 02 April 2015
Accepted: 07 April 2016
|
| Fund: This research was funded by the National Program of Transgenic Variety Development of China (2014ZX08001-001) and the China Postdoctoral Science Foundation (2013M531705). |
|
Corresponding Authors:
LIN Yong-jun, Tel: +86-27-87281719, E-mail: yongjunlin@mail.hzau.edu.cn
E-mail: yongjunlin@mail.hzau.edu.cn
|
| About author: CHEN Tai-yu, Tel: +86-27-87280516, E-mail: ctycxd@163.com |
Cite this article:
CHEN Tai-yu, HOU Ji-xiang, LIN Yong-jun.
2016.
Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler). Journal of Integrative Agriculture, 15(4): 840-847.
|
| Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.Baum J A, Bogaert T, Clinton W, Heck G R, Feldmann P, Ilagan O, Johnson S, Plaetinck G, Munyikwa T, Pleau M, Vaughn T, Roberts J. 2007. Control of coleopteran insect pests through RNA interference. Nature Biotechnology, 25, 1322–1326.Carthew R W, Sontheimer E J. 2009. Origins and mechanisms of miRNAs and siRNAs. Cell, 136, 642–655.Chen H, Tang W, Xu C, Li X, Lin Y, Zhang Q. 2005. Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests. Theoretical and Applied Genetics, 111, 1330–1337.Conesa A, Gotz S, Garcia-Gomez J M, Terol J, Talon M, Robles M. 2005. Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21, 3674–3676.Feinberg E H, Hunter C P. 2003. Transport of dsRNA into cells by the transmembrane protein SID-1. Science, 301, 1545–1547.Ferry N, Edwards M G, Gatehouse J A, Gatehouse A M. 2004. Plant-insect interactions: Molecular approaches to insect resistance. Current Opinion in Biotechnology, 15, 155–161.Firmino A A, Fonseca F C, de Macedo L L, Coelho R R, Antonino de Souza Jr J D, Togawa R C, Silva-Junior O B, Pappas-Jr G J, da Silva M C, Engler G, Grossi-de-Sa M F. 2013. Transcriptome analysis in cotton boll weevil (Anthonomus grandis) and RNA interference in insect pests. PLoS One, 8, e85079.Grabherr M G, Haas B J, Yassour M, Levin J Z, Thompson D A, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q. 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 29, 644–652.Jinek M, Doudna J A. 2009. A three-dimensional view of the molecular machinery of RNA interference. Nature, 457, 405–412.Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M. 2004. The KEGG resource for deciphering the genome. Nucleic Acids Research, 32, D277-D280.Kumar S, Tamura K, Nei M. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 5, 150–163.Li J, Chen Q, Lin Y, Jiang T, Wu G, Hua H. 2011. RNA interference in Nilaparvata lugens (Homoptera: Delphacidae) based on dsRNA ingestion. Pest Management Science, 67, 852–859.Ma W, Zhang Z, Peng C, Wang X, Li F, Lin Y. 2012. Exploring the midgut transcriptome and brush border membrane vesicle proteome of the rice stem borer, Chilo suppressalis (Walker). PLoS One, 7, e38151.Mao Y B, Cai W J, Wang J W, Hong G J, Tao X Y, Wang L J, Huang Y P, Chen X Y. 2007. Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nature Biotechnology, 25, 1307–1313.Matsumoto Y, Suetsugu Y, Nakamura M, Hattori M. 2014. Transcriptome analysis of the salivary glands of Nephotettix cincticeps (Uhler). Journal of Insect Physiology, 71, 170–176.Price D R, Gatehouse J A. 2008. RNAi-mediated crop protection against insects. Trends in Biotechnology, 26, 393–400.Senti K A, Brennecke J. 2010. The piRNA pathway: A fly’s perspective on the guardian of the genome. Trends in Genetics, 26, 499–509.Tang W, Chen H, Xu C, Li X, Lin Y, Zhang Q. 2006. Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene. Molecular Breeding, 18, 1–10.Tomizawa M, Noda H. 2013. High mortality caused by high dose of dsRNA in the green rice leafhopper Nephotettix cincticeps (Hemiptera: Cicadellidae). Applied Entomology and Zoology, 48, 553–559.Tomoyasu Y, Miller S C, Tomita S, Schoppmeier M, Grossmann D, Bucher G. 2008. Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium. Genome Biology, 9, R10.Winston W M, Molodowitch C, Hunter C P. 2002. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1. Science, 295, 2456–2459.Xu H, He X, Zheng X, Yang Y, Tian J, Lu Z. 2014. Southern rice black-streaked dwarf virus (SRBSDV) directly affects the feeding and reproduction behavior of its vector, Sogatella furcifera (Horvath) (Hemiptera: Delphacidae). Virology Journal, 11, 55.Ye J, Fang L, Zheng H, Zhang Y, Chen J, Zhang Z, Wang J, Li S, Li R, Bolund L. 2006. WEGO: A web tool for plotting GO annotations. Nucleic Acids Research, 34, W293-W297.Ye R, Huang H, Yang Z, Chen T, Liu L, Li X, Chen H, Lin Y. 2009. Development of insect-resistant transgenic rice with Cry1C*-free endosperm. Pest Management Science, 65, 1015–1020. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
