|
|
|
|
|
| Expression analysis for candidate genes associated with eggshell mechanical property |
| SUN Cong-jiao, DUaN Zhong-yi, QU Lu-jiang, ZhENg Jiang-xia, YaNg Ning, XU gui-yun |
| National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture/ College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China |
|
|
|
摘要 Damaged eggshells result in losses of eggs. The genetic mechanism of variable eggshell strength is still unclear. The current study was conducted to verify whether the eggshell calcification related genes, CALB1, SPP1, DMP4, BMP2 and SLIT2, were associated with eggshell mechanical property. For this purpose quantitative PCR (q-PCR) analysis was performed to detect gene expression between two groups of hens laying strong and weak eggs. The hens were selected from 360 White Leghorn layers at 60 wk to ensure that the strong and weak eggs differed significantly in breaking strength but not in eggshell thickness and weight. Using this special strong/weak eggshell model, we found that the expression of CALB1 in the uterus of strong shell group was about 3-fold higher (P<0.05) than that in weak shell group. The DMP4 expression was significantly higher (2-fold, P<0.05) in the uterus of weak shell group than that in strong shell group. However, no difference was observed for genes of SPP1, SLIT2 and BMP2 between these two groups. The current study provides a new insight to investigate the association of candidate genes with eggshell mechanical property.
Abstract Damaged eggshells result in losses of eggs. The genetic mechanism of variable eggshell strength is still unclear. The current study was conducted to verify whether the eggshell calcification related genes, CALB1, SPP1, DMP4, BMP2 and SLIT2, were associated with eggshell mechanical property. For this purpose quantitative PCR (q-PCR) analysis was performed to detect gene expression between two groups of hens laying strong and weak eggs. The hens were selected from 360 White Leghorn layers at 60 wk to ensure that the strong and weak eggs differed significantly in breaking strength but not in eggshell thickness and weight. Using this special strong/weak eggshell model, we found that the expression of CALB1 in the uterus of strong shell group was about 3-fold higher (P<0.05) than that in weak shell group. The DMP4 expression was significantly higher (2-fold, P<0.05) in the uterus of weak shell group than that in strong shell group. However, no difference was observed for genes of SPP1, SLIT2 and BMP2 between these two groups. The current study provides a new insight to investigate the association of candidate genes with eggshell mechanical property.
|
|
Received: 03 December 2014
Accepted:
|
| Fund: The current research was funded by the National Natural Science Foundation of China (31472084), the National High Technology Research and Development Program of China (2013AA102501), the National Key Technology R&D Program of China (2011BAD28B03), the Programs for Changjiang Scholars and Innovative Research in University, China (IRT1191), and the China Agriculture Research Systems (CARS-41). |
|
Corresponding Authors:
XU Gui-yun, Mobile: +86-13601372958,Fax: +86-10-62732741, E-mail: ncppt@cau.edu.cn
E-mail: ncppt@cau.edu.cn
|
| About author: SUN Cong-jiao, Mobile: +86-15910707895, E-mail: suncongjiao@163.com |
Cite this article:
SUN Cong-jiao, DUaN Zhong-yi, QU Lu-jiang, ZhENg Jiang-xia, YaNg Ning, XU gui-yun.
2016.
Expression analysis for candidate genes associated with eggshell mechanical property. Journal of Integrative Agriculture, 15(2): 397-402.
|
| Ahmed A M H, Rodriguez-Navarro A B, Vidal M L, GautronJ, García-Ruiz J M, Nys Y. 2005. Changes in eggshellmechanical properties, crystallographic texture and in matrixproteins induced by moult in hens. British Poultry Science,46, 268-279Arazi H, Yoselewitz I, Malka Y, Kelner Y, Genin O, Pines M.2009. Osteopontin and calbindin gene expression in theeggshell gland as related to eggshell abnormalities. PoultryScience, 88, 647-653Bain M M. 1990. Eggshell strength: A mechanical ultrastructuralevaluation. Ph D thesis, Faculty of Veterinary Medicine,University of Glasgow, UK.Bar A, Striem S, Vax E, Talpaz H, Hurwitz S. 1992a. Regulationof calbindin mRNA and calbindin turnover in intestine andshell gland of the chicken. American Journal of Physiology,262, 800-805Bar A, Vax E, Striem S. 1992b. Relationships between calbindin(Mr 28,000) and calcium transport by the eggshell gland.Comparative Biochemistry and Physiology, 101, 845-848Battye R, Stevens A, Perry R L, Jacobs J R. 2001. Repellentsignaling by slit requires the leucine-rich repeats. TheJournal of Neuroscience, 21, 4290-4298Chien Y C, Hincke M, Vali H, McKee M. 2008. Ultrastructuralmatrix-mineral relationships in avian eggshell, and effects ofosteopontin on calcite growth in vitro. Journal of StructuralBiology, 163, 84-99Chien Y C, Hincke M T, McKee M D. 2009. Ultrastructure ofavian eggshell during resorption following egg fertilization.Journal of Structural Biology, 168, 527-538Duprez D, Coltey M, Amthor H, Brickell P, Tickle C. 1996.Bone morphogenetic protein-2 (BMP-2) inhibits muscledevelopment and promotes cartilage formation in chicklimb bud cultures. Developmental Biology, 174, 448-452Gautron J, Hincke M T, Nys Y. 1997. Precursor matrix proteinsin the uterine fluid change with stages of eggshell formationin hens. Connective Tissue Research, 36, 195-210Hao J, Narayanan K, Muni T, Ramachandran A, George A.2007. Dentin matrix protein 4, a novel secretory calciumbindingprotein that modulates odontoblast differentiation.The Journal of Biological Chemistry, 282, 15357-15365Hincke M T, Chien Y C, Gerstenfeld L C, McKee M D.2008. Colloidal-gold immunocytochemical localization ofosteopontin in avian eggshell gland and eggshell. Journalof Histochemistry & Cytochemistry, 56, 467-476Jeong W, Lim W, Kim J, Ahn S E, Lee H C, Jeong J W,Han J Y, Song G, Bazer F W. 2012. Cell-specific andtemporal aspects of gene expression in the chickenoviduct at different stages of the laying cycle 1. Biology ofReproduction, 86, 172-180Jonchere V, Brionne A, Gautron J, Nys Y. 2012. Identificationof uterine ion transporters for mineralisation precursors ofthe avian eggshell. BMC Physiology, 12, 10.Jonchere V, Rehault-Godbert S, Hennequet-Antier C, CabauC, Sibut V, Cogburn L, Nys Y, Gautron J. 2010. Geneexpression profiling to identify eggshell proteins involvedin physical defense of the chicken egg. BMC Genomics,11, 57.Kobe B, Deisenhofer J. 1993. Crystal structure of porcineribonuclease inhibitor, a protein with leucine-rich repeats.Nature, 366, 751-756Lavelin I, Yarden N, Ben-Bassat S, Bar A, Pines M. 1998.Regulation of osteopontin gene expression during egg shellformation in the laying hen by mechanical strain. MatrixBiology, 17, 615-623Liu Z, Zheng Q, Zhang X, Lu L. 2013. Microarray analysisof genes involved with shell strength in layer shell gland atthe early stage of active calcification. Asian-AustralasianJournal of Animal Sciences, 26, 609-624Nys Y, Gautron J, Garcia-Ruiz J M, Hincke M T. 2004. Avianeggshell mineralization: Biochemical and functionalcharacterization of matrix proteins. Comptes RendusPalevol, 3, 549-562Nys Y, Hincke M, Arias J, Garcia-Ruiz J, Solomon S. 1999.Avian eggshell mineralization. Poultry and Avian BiologyReviews, 10, 143-166Nys Y, Mayel-Afshar S, Bouillon R, Van Baelen H, Lawson D.1989. Increases in calbindin D 28K mRNA in the uterusof the domestic fowl induced by sexual maturity and shellformation. General and Comparative Endocrinology, 76, 322-329Panheleux M, Nys Y, Williams J, Gautron J, Boldicke T, HinckeM T. 2000. Extraction and quantification by ELISA ofeggshell organic matrix proteins (ovocleidin-17, ovalbumin,ovotransferrin) in shell from young and old hens. PoultryScience, 79, 580-588Pines M. 2007. The involvement of matrix proteins in eggshellformation. In: Proceedings of the 19th Australian PoultryScience Symposium. Sydney, New South Wales, Australia.pp. 130-133Pines M, Knopov V, Bar A. 1995. Involvement of osteopontinin egg shell formation in the laying chicken. Matrix Biology,14, 765-771Preisinger R, Flock D K. 2000. Genetic Changes in LayerBreeding: Historical Trends and Future Prospects. BSASOccasional Publication, UK. pp. 20-28Rodriguez-Navarro A, Kalin O, Nys Y, Garcia-Ruiz J M. 2002.Influence of the microstructure on the shell strength of eggslaid by hens of different ages. British Poultry Science, 43,395-403Sekiya I, Larson B L, Vuoristo J T, Reger R L, Prockop D J.2005. Comparison of effect of BMP-2, -4, and -6 on in vitrocartilage formation of human adult stem cells from bonemarrow stroma. Cell and Tissue Research, 320, 269-276Sun C, Xu G, Yang N. 2013. Differential label-free quantitativeproteomic analysis of avian eggshell matrix and uterinefluid proteins associated with eggshell mechanical property.Proteomics, 13, 3523-3536Yang J, Zhao Z, Hou J, Zhou Z, Deng Y, Dai J. 2013. Expressionof TRPV6 and CaBP-D28k in the egg shell gland (uterus)during the oviposition cycle of the laying hen. British PoultryScience, 54, 398–406. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
