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Journal of Integrative Agriculture  2022, Vol. 21 Issue (8): 2357-2371    DOI: 10.1016/S2095-3119(21)63896-0
Special Issue: 动物科学合辑Animal Science
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene

SUN Yu-hang1, 2, 3, 4*, ZHAI Gui-ying1, 2, 3*, PANG Yong-jia1, 2, 3, LI Rui1, 2, 3, LI Yu-mao1, 2, 3, CAO Zhi-ping1, 2, 3, WANG Ning1, 2, 3, LI Hui1, 2, 3, WANG Yu-xiang1, 2, 3 

1  Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, P.R.China

2 Key Laboratory of Animal Genetics, Breeding and Reproduction, Heilongjiang General Colleges and Universities, Harbin 150030, P.R.China

3 College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.China

4 Fujian Sunnzer Biotechnology Development Co., Ltd., Guangze 354100, P.R.China

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Perilipin1 (PLIN1) is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets (LDs) in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol (TG).  Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptor-gamma (PPARγ), the master regulator of adipogenesis.  However, the regulatory mechanism of the chicken Plin1 (cPlin1) gene is poorly understood.  The present study aimed to investigate whether Plin1 is regulated by PPARγ in chickens and identify its exact molecular mechanism.  Reporter gene and expression assays showed that PPARγ2, but not PPARγ1, activated (P<0.01) the cPlin1 gene promoter.  An electrophoretic mobility shift assay and mutational analysis revealed that PPARγ2 bound to a special site in the cPlin1 gene promoter to enhance its expression.  In summary, our results show that PPARγ promotes the expression of the cPlin1 gene and that PPARγ2 is the main regulatory isoform.

Keywords:  chicken        PPAR gamma        isoform        Plin1        transcriptional regulation  
Received: 22 June 2021   Accepted: 24 January 2022
Fund: This work was supported by the National Natural Science Foundation of China (31201796 and 32072704), the China Agriculture Research System of MOF and MARA (CARS-41), and the Natural Science Foundation of Heilongjiang Province, China (LH2020C017).  
About author:  SUN Yu-hang, E-mail:; ZHAI Gui-ying, E-mail:; Correspondence WANG Yu-xiang, Tel: +86-451-55191495, E-mail: * These authors contributed equally to this study.

Cite this article: 

SUN Yu-hang, ZHAI Gui-ying, PANG Yong-jia, LI Rui, LI Yu-mao, CAO Zhi-ping, WANG Ning, LI Hui, WANG Yu-xiang. 2022. PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene. Journal of Integrative Agriculture, 21(8): 2357-2371.

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