Please wait a minute...
Journal of Integrative Agriculture  2015, Vol. 14 Issue (3): 561-566    DOI: 10.1016/S2095-3119(14)60832-7
Section 2: Fungi, enzymes and new developments in direct-fed microbials Advanced Online Publication | Current Issue | Archive | Adv Search |
Prospective use of bacteriocinogenic Pediococcus pentosaceus as direct-fed microbial having methane reducing potential
 Sanjay Kumar, Sumit S Dagar, Seyed H Ebrahimi, Ravinder K Malik, Ramesh C Upadhyay, AnilK Puniya
1、Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
2、Dairy Cattle Nutrition, National Dairy Research Institute, Karnal 132001, India
3、Dairy Cattle Physiology, National Dairy Research Institute, Karnal 132001, India
4、Department of Clinical Studies, School of Veterninary Medicine, University of Pennsylvania, Pennsylvania 19348, USA
5、Microbial Science Division, Agharkar Research Institute, Pune 411004, India
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  Direct-fed microbials (DFM), generally regarded as safe status, are successfully used in improving rumen ecology, gastro-intestinal health, feed efficiency, milk production and growth rate in ruminants. On the other hand, methanogenesis in rumen, which accounts for a significant loss of ruminant energy and increased greenhouse gas in environment, is of great concern, therefore, use of DFM for improving productivity without compromising the animal health and ecological sustainability is encouraged. The present study was conducted to investigate the methane reducing potential of bacteriocinogenic strain Pediococcus pentosaceus-34. Since, the culture showed no hemolysis on blood agar and DNase activity, hence, it was considered to be avirulent in nature, a prerequisite for any DFM. The culture also showed tolerance to pH 5.0 for 24 h with 0.5% organic acid mixture, whereas when given a shock for 2 h at different pH and organic acids concentrations, it showed growth at pH 3.0 and 4.0 with 0.1 and 1.0% organic acids, respectively, as having good animal probiotics attributes. The total gas production was significantly (P<0.05) higher in live pedicoccal culture (LPC) and dead pedicoccal culture (DPC) both with wheat straw, when compared to the control. In sugarcane bagasse, gas production was significantly lower (P<0.05) with LPC compared to the control and DPC both. Methane was reduced by the inclusion of LPC in sugarcane bagasse (0.07 mL CH4 mg–1 dry matter digestibility) with no effect on other rumen fermentation parameters. However, with wheat straw and LPC total gas, in vitro dry matter digestibility, total volatile fatty acids increased significantly but no reduction in methane production was observed in comparison to the control. Therefore, further research is warranted in this direction, if the bacteriocinogenic strains can be used as DFM for ruminants to improve the ruminant productivity.

Abstract  Direct-fed microbials (DFM), generally regarded as safe status, are successfully used in improving rumen ecology, gastro-intestinal health, feed efficiency, milk production and growth rate in ruminants. On the other hand, methanogenesis in rumen, which accounts for a significant loss of ruminant energy and increased greenhouse gas in environment, is of great concern, therefore, use of DFM for improving productivity without compromising the animal health and ecological sustainability is encouraged. The present study was conducted to investigate the methane reducing potential of bacteriocinogenic strain Pediococcus pentosaceus-34. Since, the culture showed no hemolysis on blood agar and DNase activity, hence, it was considered to be avirulent in nature, a prerequisite for any DFM. The culture also showed tolerance to pH 5.0 for 24 h with 0.5% organic acid mixture, whereas when given a shock for 2 h at different pH and organic acids concentrations, it showed growth at pH 3.0 and 4.0 with 0.1 and 1.0% organic acids, respectively, as having good animal probiotics attributes. The total gas production was significantly (P<0.05) higher in live pedicoccal culture (LPC) and dead pedicoccal culture (DPC) both with wheat straw, when compared to the control. In sugarcane bagasse, gas production was significantly lower (P<0.05) with LPC compared to the control and DPC both. Methane was reduced by the inclusion of LPC in sugarcane bagasse (0.07 mL CH4 mg–1 dry matter digestibility) with no effect on other rumen fermentation parameters. However, with wheat straw and LPC total gas, in vitro dry matter digestibility, total volatile fatty acids increased significantly but no reduction in methane production was observed in comparison to the control. Therefore, further research is warranted in this direction, if the bacteriocinogenic strains can be used as DFM for ruminants to improve the ruminant productivity.
Keywords:  Pediococcus pentosaceus       bacteriocin       methane       direct-fed microbials       rumen       probiotics  
Received: 21 October 2013   Accepted:
Fund: 

The work was conducted as a part of a Ph D project of Sanjay Kumar that was supported by NDRI (ICAR) fellowship. The authors are also grateful to National Initiative on Climate Resilient Agriculture, India (NICRA) for providing partial support.

Corresponding Authors:  Anil Kumar Puniya, E-mail: akpuniya@gmail.com   

Cite this article: 

Sanjay Kumar, Sumit S Dagar, Seyed H Ebrahimi, Ravinder K Malik, Ramesh C Upadhyay, AnilK Puniya. 2015. Prospective use of bacteriocinogenic Pediococcus pentosaceus as direct-fed microbial having methane reducing potential. Journal of Integrative Agriculture, 14(3): 561-566.

Abdel-Aziz N A, Salem A Z M, El-Adawy M M, Camacho LM, Kholif A E, Elghandour M M Y, Borhami B E. 2015.Biological treatments as a mean to improve feed utilizationin agriculture animals-An overview. Journal of IntegrativeAgriculture, 14, 534-543

Agarwal N, Kamra D, Chaudhary L, Sahoo A, Pathak N 2000Selection of Saccharomyces cerevisiae strains for use asa microbial feed additive. Letters in Applied Microbiology,31, 270-273

Albano H, Pinho C, Leite D, Barbosa J, Silva J, Carneiro L,Magalhães R, Hogg T, Teixeira P. 2009. Evaluation of abacteriocin-producing strain of Pediococcus acidilactici asa biopreservative. Food Control, 20, 764-770

Asa R, Tanaka A, Uehara A, Shinzato I, Toride Y, Usui N,Hirakawa K, Takahashi J. 2010. Effects of proteaseresistantantimicrobial substances produced by lactic acidbacteria on rumen methanogenesis. Asian-AustralasianJournal of Animal Science, 23, 700-707

Callaway T, Martin S, Wampler J, Hill N, Hill G. 1997. Malatecontent of forage varieties commonly fed to cattle. Journalof Dairy Science, 80, 1651-1655

Chaucheyras-Durand F, Duran H. 2010. Probiotics in animalnutrition and health. Beneficial Microbes, 1, 3-9

Elghandour M M Y, Vázquez Chagoyán J C, Salem A Z M, KholifA E, Martínez Castañeda J S, Camacho L M, Cerrillo-SotoM A. 2014. Effects of Saccharomyces cerevisiae at directaddition or pre-incubation on in vitro gas production kineticsand degradability of four fibrous feeds. Italian Journal ofAnimal Sciecne, 13, 295-301

Elghandour M M Y, Salem A Z M, Martínez Castañeda J S,Camacho L M, Kholif A E, Vázquez Chagoyán J C. 2015.Direct-fed microbes: A tool for improving the utilization oflow quality roughages in ruminants. Journal of IntegrativeAgriculture, 14, 526-533

Elsner H A, Sobottka I, Mack D, Laufs R, Claussen M, WirthR. 2000. Virulence factors of Enterococcus faecalis andEnterococcus faecium blood culture isolates. EuropeanJournal of Clinical Microbiology, 19, 39-42

Gamo Y, Mii M, Zhou X G, Sar C, Santoso B, Arai I, KimuraK,Takahashi J. 2002. Effects of lactic acid bacteria, yeastsand galactooligosaccharide supplementation on in vitrorumen methane production. In: Takahashi J, Young B A,eds., Greenhouse Gases and Animal Agriculture. ElsevierScience BV, Amsterdam, The Netherlands. pp. 201-204

Goering H K, van Soest P J. 1970. Forage fibre analysis:Apparatus, reagents, procedures and some applications.In: USDA Agricultural Handbook No. 379. US GovernmentPrinting Office, Washington, D.C. p. 20.

Gupta H, Malik R K. 2007. Incidence of virulence in bacteriocinproducingenterococcal isolates. Le Lait: Dairy Science andTechnology, 87, 587-601

Hu W L, Liu J X, Ye J A, Wu Y M, Guo Y Q. 2005. Effect oftea saponin on rumen fermentation in vitro. Animal FeedScience and Technology, 120, 333-339

Ike Y, Hashimoto H, Clewell D. 1987. High incidence ofhemolysin production by Enterococcus (Streptococcus)faecalis strains associated with human parenteral infections.Journal of Clinical Microbiology, 25, 1524-1528

Jin L, Ho Y, Abdullah N, Ali M, Jalaludin S. 1998. Effects ofadherent Lactobacillus cultures on growth, weight of organsand intestinal microflora and volatile fatty acids in broilers.Animal Feed Science and Technology, 70, 197-209

Kalmokoff M, Bartlett F, Teather R. 1996. Are ruminal bacteriaarmed with bacteriocins? Journal of Dairy Science, 79,2297-2306

Klieve A V, Hegarty R S. 1999. Opportunities for biologicalcontrol of ruminal methanogenesis. Asian-AustralasianJournal of Agricultural Research, 50, 1315-1320

Krehbiel C R, Rust S R, Zhang G, Gilliland S E. 2003. Bacterialdirect-fed microbials in ruminant diets: Performanceresponse and mode of action. Journal of Animal Science,81, E120-E132.

Kumar S, Choudhury P K, Carro M D, Griffith G W, Dagar S S,Puniya M, Calabro S, Ravella S R, Dhewa T, Upadhyay RC, Sirohi S K, Kundu S S, Wanapat M, Puniya A K. 2014.New aspects and strategies for methane mitigation fromruminants. Applied Microbiology and Biotechnology, 98,31-44

Kumar S, Dagar S S, Puniya A K, Upadhyay R C. 2013a.Changes in methane emission, rumen fermentation inresponse to dietand microbial interactions. Research inVeterinary Science, 94, 263-268

Kumar S, Dagar S S, Sirohi, S K, Upadhyay R C, Puniya AK. 2013b. Microbial profiles, in vitro gas production, drymatter digestibiltiy based on various ratio of roughage toconcentrate. Annals of Microbiology, 63, 541-545

Kumar S, Dagar S S, Puniya A K. 2012. Isolation andcharacterization of methanogens from rumen of Murrahbuffalo. Annals of Microbiology, 62, 345-350

Kumar S, Puniya A K, Puniya M, Dagar S S, Sirohi S K, SinghK, Griffith G W. 2009. Factors affecting rumen methanogensand methane mitigation strategies. World Journal ofMicrobiology and Biotechnology, 25, 1557-1566

Lee S S, Hsu J T, Mantovani H C, Russell J B. 2002. The effectof bovicin HC5, a bacteriocin from Streptococcus bovis HC5,on ruminal methane production in vitro. FEMS MicrobiologyLetters, 217, 51-55

Lila Z A, Mohammed N, Yasui T, Kurokawa Y, Kanda S,Itabashi H. 2004. Effects of a twin strain of Saccharomycescerevisiae live cells on mixed ruminal microorganismfermentation in vitro. Journal of Animal Science, 82,1847-1854

Martin S A, Nisbet D J. 1992. Effect of direct-fed microbials onrumen microbial fermentation. Journal of Dairy Science,75, 1736-1744

 Newbold C J, Wallace R J, Chen X B, McIntosh F M. 1995.Different strains of Saccharomyces cerevisiae differ in theireffects on ruminal bacterial numbers in vitro and in sheep.Journal of Animal Science, 73, 1811-1818

Nollet L, Mbanzamihigo L, Demeyer D, Verstraete W. 1998.Effect of the addition of Peptostreptococcus productusATCC 35244 on reductive acetogenesis in the ruminalecosystem after inhibition of methanogenesis by cell-freesupernatant of Lactobacillus plantarum 80. Animal FeedScience and Technology, 71, 49-66

Puniya A K, Salem A Z M, Kumar S, Dagar S S, Griffith G W,Puniya M, Ravella S R, Kumar N, Dhewa T, Kumar R. 2015.Role of live microbial feed supplements with reference toanaerobic fungi in ruminant productivity: A review. Journalof Integrative Agriculture, 14, 550-560

Russell J B, Mantovani H C. 2002. The bacteriocins of ruminalbacteria and their potential as an alternative to antibiotics.Journal of Molecular Microbiology and Biotechnology, 4, 347-355

Sandri M, Manfrin C, Pallavicini A, Stefanon B. 2014. Microbialdiversity of the liquid fraction of rumen content from lactatingcows. Animal, 8, 572-579

Tabe E S, Oloya J, Doetkott D K, Bauer M L, Gibbs P S,Khaitsa M L. 2008. Comparative effect of direct-fedmicrobials on fecal shedding of Escherichia coli O157:H7and Salmonella in naturally infected feedlot cattle. Journalof Food Protection, 71, 539-544

Tilley J M A, Terry R A. 1963. A two-stage technique for the invitro digestion of forage crops. Grass and Forage Science,18, 104-111

Weinberg Z, Shatz O, Chen Y, Yosef E, Nikbahat M, Ben-Ghedalia D, Miron J. 2007. Effect of lactic acid bacteriainoculants on in vitro digestibility of wheat and corn silages.Journal of Dairy Science, 90, 4754-4762

Wilkinson L. 1992. SYSTAT for Windows: Statistics, Graphics,Data, Getting Started. Version 5. Systat, USA.
[1] Weiwei Wang, Wei Guo, Jianxin Jiao, Emilio M Ungerfeld, Xiaoping Jing, Xiaodan Huang, Allan A Degen, Yu Li, Sisi Bi, Ruijun Long. Effects of ratios of yak to cattle inocula on methane production and fiber digestion in rumen in vitro cultures[J]. >Journal of Integrative Agriculture, 2025, 24(4): 1270-1284.
[2] Haokai Ma, Dengke Liu, Rui Liu, Yang Li, Modinat Tolani Lambo, Baisheng Dai, Weizheng Shen, Yongli Qu, Yonggen Zhang. 16S amplicon sequencing and untargeted metabolomics reveal changes in rumen microorganisms and metabolic pathways involved in the reduction of methane by cordycepin[J]. >Journal of Integrative Agriculture, 2025, 24(4): 1310-1326.
[3] Shakoor Abdul, Zaib Gul, Ming Xu. Tracing the contribution of cattle farms to methane emissions through bibliometric analyses[J]. >Journal of Integrative Agriculture, 2025, 24(4): 1220-1233.
[4] Yunlong Liu, Mi Zhou, Qiyu Diao, Tao Ma, Yan Tu. Seaweed as a feed additive to mitigate enteric methane emissions in ruminants: Opportunities and challenges[J]. >Journal of Integrative Agriculture, 2025, 24(4): 1327-1341.
[5] Hairen Shi, Pei Guo, Jieyan Zhou, Zhen Wang, Meiyue He, Liyuan Shi, Xiaojuan Huang, Penghui Guo, Zhaoxia Guo, Yuwen Zhang, Fujiang Hou. Effects of stocking rate on growth performance, energy and nitrogen utilization, methane emission, and grazing behavior in Tan sheep grazed on typical steppe[J]. >Journal of Integrative Agriculture, 2025, 24(4): 1234-1245.
[6] Wenjuan Li, Tao Ma, Naifeng Zhang, Kaidong Deng, Qiyu Diao. Dietary fat supplement affected energy and nitrogen metabolism efficiency and shifted rumen fermentation toward glucogenic propionate production via enrichment of Succiniclasticum in male twin lambs[J]. >Journal of Integrative Agriculture, 2025, 24(4): 1285-1295.
[7] Yajun Zhang, Xiao Chang, Bing Wang, Dawei Wei, Rongzhen Zhong, Yansheng Guo, Min Du, Guijie Zhang. Supplementation of Lycium barbarum residue increases the growth rate of Tan sheep by enhancing their feed intake and regulating their rumen microbiome and metabolome[J]. >Journal of Integrative Agriculture, 2024, 23(9): 3129-3144.
[8] Wenqiang Wang, Xizhen Guan, Yong Gan, Guojun Liu, Chunhao Zou, Weikang Wang, Jifa Zhang, Huifei Zhang, Qunqun Hao, Fei Ni, Jiajie Wu, Lynn Epstein, Daolin Fu.

Creating large EMS populations for functional genomics and breeding in wheat [J]. >Journal of Integrative Agriculture, 2024, 23(2): 484-493.

[9] GUO Yun-xia, YANG Ruo-chen, DUAN Chun-hui, WANG Yong, HAO Qing-hong, JI Shou-kun, YAN Hui, ZHANG Ying-jie, LIU Yue-qin. Effect of dioscorea opposite waste on growth performance, blood parameters, rumen fermentation and rumen bacterial community in weaned lambs[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1833-1846.
[10] HUANG Wen-qin, CUI Kai, HAN Yong, CHAI Jian-min, WANG Shi-qin, LÜ Xiao-kang, DIAO Qi-yu, ZHANG Nai-feng. Long term effects of artificial rearing before weaning on the growth performance, ruminal microbiota and fermentation of fattening lambs[J]. >Journal of Integrative Agriculture, 2022, 21(4): 1146-1160.
[11] DONG Li-feng, JIA Peng, LI Bin-chang, WANG Bei, YANG Chun-lei, LIU Zhi-hao, DIAO Qi-yu. Quantification and prediction of enteric methane emissions from Chinese lactating Holstein dairy cows fed diets with different dietary neutral detergent fiber/non-fibrous carbohydrate (NDF/NFC) ratios[J]. >Journal of Integrative Agriculture, 2022, 21(3): 797-811.
[12] YIN Xue-jiao, JI Shou-kun, DUAN Chun-hui, TIAN Pei-zhi, JU Si-si, YAN Hui, ZHANG Ying-jie, LIU Yue-qin. Dynamic change of fungal community in the gastrointestinal tract of growing lambs[J]. >Journal of Integrative Agriculture, 2022, 21(11): 3314-3328.
[13] Zhang Hao, Cheng Xuan, Mabrouk ELSABAGH, Lin Bo, Wang Hong-rong. Effects of formic acid and corn flour supplementation of banana pseudostem silages on nutritional quality of silages, growth, digestion, rumen fermentation and cellulolytic bacterial community of Nubian black goats[J]. >Journal of Integrative Agriculture, 2021, 20(8): 2214-2226.
[14] TONG Jin-jin, ZHANG Hua, WANG Jia, LIU Yun, MAO Sheng-yong, XIONG Ben-hai, JIANG Lin-shu. Effects of different molecular weights of chitosan on methane production and bacterial community structure in vitro[J]. >Journal of Integrative Agriculture, 2020, 19(6): 1644-1655.
[15] PENG Quan-hui, CHENG Long, KANG Kun, Tian Gang, Mohammad AL-MAMUN, XUE Bai, WANG Li-zhi, ZOU Hua-wei, Mathew Gitau GICHEHA, WANG Zhi-sheng. Effects of yeast and yeast cell wall polysaccharides supplementation on beef cattle growth performance, rumen microbial populations and lipopolysaccharides production[J]. >Journal of Integrative Agriculture, 2020, 19(3): 810-819.
No Suggested Reading articles found!