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Special Issue:
动物营养合辑Animal Nutrition
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| Long term effects of artificial rearing before weaning on the growth performance, ruminal microbiota and fermentation of fattening lambs |
| HUANG Wen-qin1, CUI Kai1, HAN Yong2, CHAI Jian-min1, 3, WANG Shi-qin1, LÜ Xiao-kang1, DIAO Qi-yu1, ZHANG Nai-feng1 |
1 Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, P.R.China
2 Guizhou Institute of Animal Husbandry and Veterinary Science, Guizhou 550005, P.R.China
3 Department of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, AR, 72701, USA |
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摘要
早期营养干预是调控成年动物肠道微生物区系的重要驱动力。然而，对于羔羊早期营养调控产生后效应的机制还没有统一的认识。本研究将60只20日龄羔羊分为随母哺乳（ER）和人工饲养（AR）两组，研究了AR策略对断奶前（20-60日龄）羔羊瘤胃微生物区系、发酵功能和组织形态的影响及其在育肥期（61-180日龄）的后效应。在断奶前阶段，ER羔羊随母哺乳并补饲开食料，AR羔羊则用代乳粉和开食料人工喂养。在育肥阶段，两个处理的所有羔羊均饲喂相同的育肥日粮。在60、120和180日龄时，每组分别屠宰6只羔羊，采集瘤胃内容物和组织样品以备检测相关指标。与ER羔羊相比，AR羔羊20-180日龄干物质采食量显著增加（P<0.05），61-120日龄日增重显著提高（P<0.05），121-180日龄日增重呈上升趋势（0.05<P<0.1）。虽然断奶前羔羊瘤胃短链脂肪酸（SCFA，包括乙酸、丙酸和丁酸）组间无显著差异（P>0.05），但育肥全期AR羔羊的瘤胃SCFA显著高于ER羔羊（P<0.05）。试验全期AR羔羊瘤胃角质层厚度低于ER羔羊（P<0.05）。随着羔羊从60-180日龄的生长，AR和ER组羔羊的瘤胃细菌多样性差异显著变大（P<0.05）。与ER羔羊相比，AR羔羊60日龄瘤胃螺旋体门（Spirochaetes）和密螺旋体属（Treponema）、120日龄瘤胃放线杆菌门（Actinobacteria）和琥珀酸杆菌属（Succiniclasticum）及180日龄瘤胃变形杆菌门（Proteobacteria）菌群相对丰度显著提高（P<0.05），但60-180日龄瘤胃硒单胞菌属（Selenomonas）、180日龄时瘤胃厌氧弧菌属（Anaerovibrio）菌群相对丰度显著降低（P<0.05）。综上所述，断奶前早期干预通过提高羔羊干物质采食量，促进了瘤胃发育、对瘤胃发酵产生积极的后效应，对肥育羔羊增重性能也产生了一定的后效作用。建议利用早期人工饲养策略改善育肥羔羊瘤胃发酵功能。
Abstract Early life intervention is important to shape the gut microbiome profiles of adult animals due to the tremendous alteration of diet components. Nevertheless, there is still no unified understanding about its long-term effects in lambs. In this study, sixty 20-day-old lambs were assigned into ewe-rearing (ER) and artificial-rearing (AR) treatments to evaluate the effects of AR strategy on ruminal microbiota, fermentation, and morphology of pre-weaning lambs (from 20 to 60 days of age) and its long-term effects in the fattening stage (from 61 to 180 days of age). During the pre-weaning stage, ER lambs were breastfed and supplemented starter, while AR lambs were artificially fed with milk replacer and starter. During the fattening stage, all lambs in both treatments were fed with the same fattening diets. At 60, 120 and 180 days of age, 6 lambs from each group were slaughtered to collect rumen content and tissue samples. Compared with ER lambs, the dry matter feed intakes of AR lambs increased (P<0.05) from 20 to 180 days of age, companying an increased average daily gain (ADG) from 61 to 120 days of age (P<0.05) and from 121 to 180 days of age (0.05<P<0.1). Although there was no difference in short-chain fatty acid (SCFA, including acetate, propionate, and butyrate) between treatments before weaning (P>0.05), it was higher (P<0.05) in AR lambs compared with ER lambs at the fattening stage. The rumen keratin layer of AR lambs was thinner (P<0.05) than that of ER lambs. Along with lamb growth from 60 to 180 days of age, the differences in rumen bacterial diversity between AR and ER treatments grew more distinct (P<0.05). Compared with ER lambs, AR lambs increased (P<0.05) rumen bacteria abundance, such as phylum Spirochaetes and genus Treponema at 60 days of age, phylum Actinobacteria and genus Succiniclasticum at 120 days of age, and phylum Proteobacteria at 180 days of age, but decreased genus Selenomonas from 60 to 180 days of age, and Anaerovibrio at 180 days of age. In summary, the early interventions before weaning could improve dry matter feed intake of lambs, which triggered robust rumen development and produced positive long-term effects on rumen fermentation and noticeable weight gain of fattening lambs. It suggests that the artificial rearing strategy is effective in improving rumen fermentation and microbial maturity of intensive fattening lambs.
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Received: 30 August 2020
Accepted: 21 June 2021
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| Fund: This research was funded by the National Natural Science Foundation of China (31872385), the Fundamental Research Funds for Central Non-profit Scientific Institution, China (Y2019CG08) and the National Key R&D Program of China (2017YFD0502001). |
| About author: HUANG Wen-qin, E-mail: 1181288617@qq.com; Correspondence ZHANG Nai-feng, E-mail: zhangnaifeng@caas.cn |
Cite this article:
HUANG Wen-qin, CUI Kai, HAN Yong, CHAI Jian-min, WANG Shi-qin, LÜ Xiao-kang, DIAO Qi-yu, ZHANG Nai-feng.
2022.
Long term effects of artificial rearing before weaning on the growth performance, ruminal microbiota and fermentation of fattening lambs. Journal of Integrative Agriculture, 21(4): 1146-1160.
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Abecia L, Martín-García A I, Martínez G, Newbold C J, Yáñez-Ruiz D R. 2013. Nutritional intervention in early life to manipulate rumen microbial colonization and methane output by kid goats postweaning. Journal of Animal Science, 91, 4832–4840.
Abecia L, Ramos-Morales E, Martínez-Fernandez G, Arco A, Martín-García A I, Newbold C J, Yáñez-Ruiz D R. 2014a. Feeding management in early life influences microbial colonisation and fermentation in the rumen of newborn goat kids. Animal Production Science, 54, 1449.
Abecia L, Waddams K E, Martínez-Fernandez G, Martín-García A I, Ramos-Morales E, Newbold C J, Yáñez-Ruiz D R. 2014b. An antimethanogenic nutritional intervention in early life of ruminants modifies ruminal colonization by Archaea. Archaea, 2014, 841463.
AOAC (Association of Official Analytical Chemists). 2010. Official Methods of Analysis. Association of Official Analytical Chemists, Washington, D.C., USA.
Belanche A, Cooke J, Jones E, Worgan H J, Newbold C J. 2019a. Short- and long-term effects of conventional and artificial rearing strategies on the health and performance of growing lambs. Animal, 13, 740–749.
Belanche A, Newbold C J, Lin W, Rees Stevens P, Kingston-Smith, A H. 2017. A systems biology approach reveals differences in the dynamics of colonization and degradation of grass vs. hay by rumen microbes with minor effects of vitamin E supplementation. Frontiers in Microbiology, 8, 1456.
Belanche A, Yáñez Ruiz D R, Detheridge A P, Griffith G W, Kingston Smith A H, Newbold C J. 2019b. Maternal versus artificial rearing shapes the rumen microbiome having minor long-term physiological implications. Environmental Microbiology, 21, 4360–4377.
Bhatt R S, Tripathi M K, Verma D L, Karim S A. 2009. Effect of different feeding regimes on pre-weaning growth rumen fermentation and its influence on post-weaning performance of lambs. Journal of Animal Physiology and Animal Nutrition, 93, 568–576.
Carmody R N, Gerber G K, Luevano J M, Jr Gatti D M, Somes L, Svenson K L, Turnbaugh P J. 2015. Diet dominates host genotype in shaping the murine gut microbiota. Cell Host & Microbe, 17, 72–84.
Chai J, Wang B, QI M, Wang S, Tu Y, Tao X, Diao Q, Zhang N. 2018. Effect of weaning liquid diet at different level of creep feed intake on growth and development of lambs. Scientia Agricultura Sinica, 51, 341–350. (in Chinese)
Chai J M, Ma T, Wang H C, Qi M L, Tu Y, Diao Q Y, Zhang N F. 2017. Effect of early weaning age on growth performance, nutrient digestibility, and serum parameters of lambs. Animal Production Science, 57, 110–115.
Chaney A L, Marbach E P. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry, 8, 130–132.
Connor E E, Baldwin R L T, Li C J, Li R W, Chung H. 2013. Gene expression in bovine rumen epithelium during weaning identifies molecular regulators of rumen development and growth. Functional and Integrative Genomics, 13, 133–142.
Cui X, Wang Z, Yan T, Chang S, Wang H, Hou F. 2019. Rumen bacterial diversity of Tibetan sheep (Ovis aries) associated with different forage types on the Qinghai–Tibetan Plateau. Canadian Journal of Microbiology, 65, 859–869.
Fernando S C, Purvis H T, Najar F Z, Sukharnikov L O, Krehbiel, C R, Nagaraja T G, Roe B A, Desilva U. 2010. Rumen microbial population dynamics during adaptation to a high-grain diet. Applied and Environmental Microbiology, 76, 7482–7490.
Haschke F, Grathwohl D, Detzel P, Steenhout P, Wagemans N, Erdmann P. 2016. Postnatal high protein intake can contribute to accelerated weight gain of infants and increased obesity risk. Nestle Nutrition Institute Workshop Series, 85, 101–109.
Lesmeister K E, Tozer P R, Heinrichs A J. 2004. Development and analysis of a rumen tissue sampling procedure. Journal of Dairy Science, 87, 1336–1344.
Li C, Wang W, Liu T, Zhang Q, Wang G, Li F, Li F, Yue X, Li T. 2018. Effect of early weaning on the intestinal microbiota and expression of genes related to barrier function in lambs. Frontiers in Microbiology, 9, 1431.
Li R W, Connor E E, Li C, Baldwin VI R L, Sparks M E. 2012. Characterization of the rumen microbiota of pre-ruminant calves using metagenomic tools. Environmental Microbiology, 14, 129–139.
Liang T G. 2012. Research on lactation rules of Shanbei white cashmere goat and weight growth rules of lambs during lactation period. MSc thesis, Northwest A&F University, Shaanxi. (in Chinese)
Liu T, Li F, Wang W, Yue X, Li F, Li C, Pan X, Mo F, Wang F, La Y, Li B. 2016. Effects of lamb early starter feeding on the expression of genes involved in volatile fatty acid transport and pH regulation in rumen tissue. Animal Feed Science and Technology, 217, 27–35.
Lv X, Chai J, Diao Q, Huang W, Zhuang Y, Zhang N. 2019. The signature microbiota drive rumen function shifts in goat kids introduced to solid diet regimes. Microorganisms, 7, 516.
Malmuthuge N, Griebel P J, Guan le L. 2014. Taxonomic identification of commensal bacteria associated with the mucosa and digesta throughout the gastrointestinal tracts of preweaned calves. Applied and Environmental Microbiology, 80, 2021–2028.
Malmuthuge N, Guan L L. 2017. Understanding host–microbial interactions in rumen: Searching the best opportunity for microbiota manipulation. Journal of Animal Science and Biotechnology, 8, 8.
Meale S J, Li S, Azevedo P, Derakhshani H, Plaizier J C, Khafipour E, Steele M A. 2016. Development of ruminal and fecal microbiomes are affected by weaning but not weaning strategy in dairy calves. Frontiers in Microbiology, 7, 582.
Meale S J, Li S C, Azevedo P, Derakhshani H, DeVries T J, Plaizier J C, Steele M A, Khafipour E. 2017. Weaning age influences the severity of gastrointestinal microbiome shifts in dairy calves. Scientific Reports, 7, 198.
Myer P R, Freetly H C, Wells J E, Smith T P L, Kuehn L A. 2017. Analysis of the gut bacterial communities in beef cattle and their association with feed intake, growth, and efficiency. Journal of Animal Science, 95, 3215–3224.
Napolitano F, Cifuni G F, Pacelli C, Riviezzi A M, Girolami A. 2002. Effect of artificial rearing on lamb welfare and meat quality. Meat Science, 60, 307–315.
Napolitano F, De Rosa G, Sevi A. 2008. Welfare implications of artificial rearing and early weaning in sheep. Applied Animal Behaviour Science, 110, 58–72.
NY/T 816-2004. 2004. Feeding standard of meat-producing sheep and goats. Agricultural Industry Standards of the People’s Republic of China. (in Chinese)
Paz H A, Anderson C L, Muller M J, Kononoff P J, Fernando S C. 2016. Rumen bacterial community composition in Holstein and Jersey cows is different under same dietary condition and is not affected by sampling method. Frontiers in Microbiology, 7, 1206.
Petri R M, Schwaiger T, Penner G B, Beauchemin K A, Forster R J, McKinnon J J, McAllister T A. 2013. Characterization of the core rumen microbiome in cattle during transition from forage to concentrate as well as during and after an acidotic challenge. PLoS ONE, 8, e83424.
Qian W, Ao W, Hui X, Wu J. 2018. Lower dietary concentrate level increases bacterial diversity in the rumen of Cervus elaphus yarkandensis. Canadian Journal of Microbiology, 64, 501–509.
Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett W. 2011. Metagenomic biomarker discovery and explanation. Genome Biology, 12, R60.
Van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597.
Sun D M, Mao S Y, Zhu W Y, Liu J H. 2018. Effect of starter diet supplementation on rumen epithelial morphology and expression of genes involved in cell proliferation and metabolism in pre-weaned lambs. Animal, 12, 2274–2283.
Wang W, Li C, Li F, Wang X, Zhang X, Liu T, Nian F, Yue X, Li F, Pan X, La Y, Mo F, Wang F, Li B. 2016. Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs. Scientific Reports, 6, 32479.
Xie B, Huang W, Zhang C, Diao Q, Cui K, Chai J, Wang S, Lv X, Zhang N. 2020. Influences of starter NDF level on growth performance and rumen development in lambs fed isocaloric and isonitrogenous diets. Journal of Animal Science, 98, skaa093.
Xie X, Yang C, Guan L L, Wang J, Xue M, Liu J X. 2018. Persistence of cellulolytic bacteria fibrobacter and treponema after short-term corn stover-based dietary intervention reveals the potential to improve rumen fibrolytic function. Frontiers in Microbiology, 9, 1363.
Xiong B H, Pang Z H, Luo Q Y. 2012. Tables of feed composition and nutritive values in China (23rd edition, 2012). China Feed, 473, 33–34. (in Chinese)
Yáñez-Ruiz D R, Leticia A, Newbold C J. 2015. Manipulating rumen microbiome and fermentation through interventions during early life: A review. Frontiers in Microbiology, 6, 1133.
Yáñez-Ruiz D R, Macías B, Pinloche E, Charles J. Newbold C J. 2010. The persistence of bacterial and methanogenic archaeal communities residing in the rumen of young lambs. FEMS Microbiology Ecology, 72, 272–278.
Zhang L, Chung J, Jiang Q, Sun R, Zhang J, Zhong Y, Ren N. 2017. Characteristics of rumen microorganisms involved in anaerobic degradation of cellulose at various pH values. RSC Advances, 7, 40303–40310.
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