|
|
|
|
|
| Comparative Analysis of Gene Expression Profiling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max |
| LI Bin, SUN Jun-ming, WANG Lan, ZHAO Rong-juan , WANG Lian-zheng |
| The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Soybean Biology (Beijing), MOA/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
|
|
|
摘要 Soybean cyst nematode (SCN) is one of the most devastating pathogen for soybean. Therefore, identification of resistant germplasm resources and resistant genes is needed to improve SCN resistance for soybean. Soybean varieties Huipizhiheidou and Wuzhaiheidou were distributed in China and exhibited broad spectrums of resistance to various SCN races. In this study, these two resistant varieties, combined with standard susceptible varieties (Lee and Essex), were utilized to identify the differentially expressed transcripts after infection with SCN race 4 between resistant and susceptible reactions by using the Affymetrix Soybean Genome GeneChip. Comparative analyses indicated that 21 common genes changed significantly in the resistant group, of which 16 increased and 5 decreased. However, 12 common genes changed significantly in the susceptible group, of which 9 increased and 3 decreased. Additionally, 27 genes were found in common between resistant and susceptible reactions. The 21 significantly changed genes in resistant reaction were associated with disease and defense, cell structure, transcription, metabolism, and signal transduction. The fold induction of 4 from the 21 genes was confirmed by quantitative RT-PCR (qRTPCR) analysis. Moreover, the gene ontology (GO) enrichment analyses demonstrated the serine family amino acid metabolic process and arginine metabolic process may play important roles in SCN resistance. This study provided a new insight on the genetic basis of soybean resistance to SCN race 4, and the identified resistant or resistant-related genes are potentially useful for SCN-resistance breeding in soybean.
Abstract Soybean cyst nematode (SCN) is one of the most devastating pathogen for soybean. Therefore, identification of resistant germplasm resources and resistant genes is needed to improve SCN resistance for soybean. Soybean varieties Huipizhiheidou and Wuzhaiheidou were distributed in China and exhibited broad spectrums of resistance to various SCN races. In this study, these two resistant varieties, combined with standard susceptible varieties (Lee and Essex), were utilized to identify the differentially expressed transcripts after infection with SCN race 4 between resistant and susceptible reactions by using the Affymetrix Soybean Genome GeneChip. Comparative analyses indicated that 21 common genes changed significantly in the resistant group, of which 16 increased and 5 decreased. However, 12 common genes changed significantly in the susceptible group, of which 9 increased and 3 decreased. Additionally, 27 genes were found in common between resistant and susceptible reactions. The 21 significantly changed genes in resistant reaction were associated with disease and defense, cell structure, transcription, metabolism, and signal transduction. The fold induction of 4 from the 21 genes was confirmed by quantitative RT-PCR (qRTPCR) analysis. Moreover, the gene ontology (GO) enrichment analyses demonstrated the serine family amino acid metabolic process and arginine metabolic process may play important roles in SCN resistance. This study provided a new insight on the genetic basis of soybean resistance to SCN race 4, and the identified resistant or resistant-related genes are potentially useful for SCN-resistance breeding in soybean.
|
|
Received: 19 November 2013
Accepted:
|
| Fund: This work was supported by the National Nature Science Foundation of China (31301345 and 31171576), the CAAS Innovation Project, the Genetically Modified Organisms Breeding Major Projects, China (2009ZX08004-003B and 2011ZX08004-003), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD35B06-3). |
|
Corresponding Authors:
WANG Lian-zheng, Tel: +86-10-8210-3007, Fax: +86-10-82105805, E-mail: wanglianzheng@caas.cn
E-mail: wanglianzheng@caas.cn
|
| About author: LI Bin, E-mail: libin02@caas.cn,These authors contributed equally to this work. |
Cite this article:
LI Bin, SUN Jun-ming, WANG Lan, ZHAO Rong-juan , WANG Lian-zheng.
2014.
Comparative Analysis of Gene Expression Profiling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max. Journal of Integrative Agriculture, 13(12): 2594-2607.
|
| Alkharouf N W, Klink V P, Chouikha I B, Beard H S,MacDonald M H, Meyer S, Knap H T, Khan R, MatthewsB F. 2006. Timecourse microarray analyses reveal globalchanges in gene expression of susceptible Glycine max(soybean) roots during infection by Heterodera glycines(soybean cyst nematode). Planta, 224, 838-852Arelli A P, Wilcox J A, Myers Jr O, Gibson P T. 1997. Soybeangermplasm resistant to races 1 and 2 of Heteroderaglycines. Crop Science, 37, 1367-1369Bustin S A. 2002. Quantification of mRNA using realtimereverse transcription PCR (RT-PCR): Trends andproblems. Journal of Molecular Endocrinology, 29, 23-29Cai D, Kleine M, Kifle S, Harloff H J, Sandal N N, MarckerK A, Klein-Lankhorst R M, Salentijn E M, Lange W,Stiekema W J, Wyss U, Grundler F M, Jung C. 1997.Positional cloning of a gene for nematode resistance insugar beet. Science, 275, 832-834Caldwell B E, Brim C A, Ross J P. 1960. Inheritance ofresistance to soybean cyst nematode, Heterodera glycines.Agronomy Journal, 52, 635-636Cook D E, Lee T G, Guo X, Melito S, Wang K, Bayless A M,Wang J, Hughes T J, Willis D K, Clemente T E, Diers BW, Jiang J, Hudson M E, Bent A F. 2012. Copy numbervariation of multiple genes at Rhg1 mediates nematoderesistance in soybean. Science, 338, 1206-1209Du Z, Zhou X, Ling Y, Zhang Z, Su Z. 2010. AgriGO: A GOanalysis toolkit for the agricultural community. NucleicAcids Research, 38, W64-W70.Duan Y, Zhou B, Chen L, Wu H. 2008. Representative analysis of the estabolishment of a core collection focused on race3 of soybean cyst nematode. Soybean Science, 27, 366-372 (in Chinese)Endo B Y. 1965. Histological responses of resistantand susceptible soybean varieties, and backcrossprogeny to entry development of Heterodera glycines.Phytopathology, 55, 375-381Hwang C F, Bhakta A V, Truesdell G M, Pudlo W M,Williamson V M. 2000. Evidence for a role of the Nterminus and leucine-rich repeat region of the Mi geneproduct in regulation of localized cell death. The PlantCell, 12, 1319-1329Ithal N, Recknor J, Nettleton D, Hearne L, Maier T, Baum TJ, Mitchum M G. 2007a. Parallel genome-wide expressionprofiling of host and pathogen during soybean cystnematode infection of soybean. Molecular Plant-MicrobeInteractions, 20, 293-305Ithal N, Recknor J, Nettleton D, Maier T, Baum T J, MitchumM G. 2007b. Developmental transcript profiling of cystnematode feeding cells in soybean roots. Molecular Plant-Microbe Interactions, 20, 510-525Jones J T, Furlanetto C, Phillips M S. 2007. The role offlavonoids produced in response to cyst nematode infectionof Arabidopsis thaliana. Nematology, 9, 671-677Kim Y H, Riggs R D, Kim K S. 1987. Structural changesassociated with resistance of soybean to Heteroderaglycines. The Journal of Nematology, 19, 177-187Klink V P, Hosseini P, MacDonald M H, Alkharouf N W,Matthews B F. 2009a. Population-specific gene expressionin the plant pathogenic nematode Heterodera glycinesexists prior to infection and during the onset of a resistantor susceptible reaction in the roots of the Glycine maxgenotype Peking. BMC Genomics, 10, 111.Klink V P, Hosseini P, Matsye P, Alkharouf N W, MatthewsB F. 2009b. A gene expression analysis of syncytia lasermicrodissected from the roots of the Glycine max (soybean)genotype PI 548402 (Peking) undergoing a resistantreaction after infection by Heterodera glycines (soybeancyst nematode). Plant Molecular Biology, 71, 525-567Klink V P, Overall C C, Alkharouf N W, MacDonald MH, Matthews B F. 2007a. A time-course comparativemicroarray analysis of an incompatible and compatibleresponse by Glycine max (soybean) to Heterodera glycines(soybean cyst nematode) infection. Planta, 226, 1423-1447Klink V P, Overall C C, Alkharouf N W, MacDonald M H,Matthews B F. 2007b. Laser capture microdissection(LCM) and comparative microarray expression analysis ofsyncytial cells isolated from incompatible and compatiblesoybean (Glycine max) roots infected by the soybean cystnematode (Heterodera glycines). Planta, 226, 1389-1409Klink V P, Overall C C, Alkharouf N W, Macdonald M H,Matthews B F. 2010. Microarray detectioncall methodologyas a means to identify and compare transcripts expressedwithin syncytial cells from soybean (Glycine max) rootsundergoing resistant and susceptible reactions to the soybean cyst nematode (Heterodera glycines). Journal ofBiomedicine and Biotechnology, 2010, 491217.Koenning S R, Wrather J A. 2010. Suppression of soybeanyield potential in the continental United States fromplant diseases estimated from 2006 to 2009. Plant HealthProgress. http://dx.doi.org/10.1094/PHP-2010-1122-01-RSLi Y, Qi X, Chang R, Qiu L. 2011. Evaluation and utilizationof soybean germplasm for resistance to cyst nematode inChina. In: Sudaric A ed., Soybean-Molecular Aspects ofBreeding. InTech, Croatia. pp. 373-396Li Y, Wang Z, Jiao G, Chang R. 1991. Studies on resistanceof soybean germplasm resources to race 4 of soybeancyst nematode. Scientia Agricultura Sinica, 24, 64-69.(in Chinese)Liu S, Kandoth P K, Warren S D, Yeckel G, Heinz R, Alden J,Yang C, Jamai A, El-Mellouki T, Juvale P S, Hill J, BaumT J, Cianzio S, Whitham S A, Korkin D, Mitchum M G,Meksem K. 2012. A soybean cyst nematode resistancegene points to a new mechanism of plant resistance topathogens. Nature, 492, 256-260Liu Y, Dammann C, Bhattacharyya M K. 2001. The matrixmetalloproteinase gene GmMMP2 is activated in responseto pathogenic infections in soybean. Plant Physiology,127, 1788-1797Maleck K, Levine A, Eulgem T, Morgan A, Schmid J, LawtonK A, Dangl J L, Dietrich R A. 2000. The transcriptome ofArabidopsis thaliana during systemic acquired resistance.Nature Genetics, 26, 403-410Matson A L, Williams L F. 1965. Evidence of a fourth gene forresistance to soybean cyst nematode. Crop Science, 5, 477.Matthews B F, Beard H, MacDonald M H, Kabir S, Youssef RM, Hosseini P, Brewer E. 2013. Engineered resistance andhypersusceptibility through functional metabolic studies of100 genes in soybean to its major pathogen, the soybeancyst nematode. Planta, 237, 1337-1357Mazarei M, Liu W, AI-Ahmad H, Arelli P R, Pantalone V R,Stewart Jr C N. 2011. Geneexpression profiling of resistantand susceptible soybean lines infected with soybean cystnematode. Theoretical and Applied Genetics, 123, 1193-1206Ndamukong I, Abdallat A A, Thurow C, Fode B, ZanderM, Weigel R, Gatz C. 2007. SA-inducible Arabidopsisglutaredoxin interacts with TGA factors and suppressesJA-responsive PDF1.2 transcription. The Plant Journal,50, 128-139Rao-Arelli A P, Anand S C, Wrather A J. 1992. Soybeanresistance to soybean cyst nematode race 3 is conditionedby an additional dominant gene. Crop Science, 32, 862-864Schmitt D P, Shannon G. 1992. Differentiating soybeanresponses to Heterodera Glycines races. Crop Science,32, 275-277Shen Q H, Saijo Y, Mauch S, Biskup C, Bieri S, Keller B,Seki H, Ulker B, Somssich I E, Schulze-Lefert P. 2007.Nuclear activity of MLA immune receptors links isolatespecificand basal disease-resistance responses. Science,315, 1098-1103Wuyts N, Lognay G, Swennen R, de Waele D. 2006. Nematodeinfection and reproduction in transgenic and mutantArabidopsis and tobacco with an altered phenylpropanoidmetabolism. Journal of Experimental Botany, 57, 2825-2835Yan Q, Chen P, Wang L. 1996. Mechanism of resistanceto race 4 of Heterodera Glycines in Chinese blacksoybeans.1. The effects of resistant varities on thepenetration and devolepment of Heterodera glycines. ActaPhytopathologica Sinica, 26, 317-323. (in Chinese)Zander M, Chen S, Imkampe J, Thurow C, Gatz C. 2012.Repression of the Arabidopsis thaliana jasmonic acid/ethylene-induced defense pathway by TGA-interactingglutaredoxins depends on their C-terminal ALWL motif.Molecular Plant, 5, 831-840 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
