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Journal of Integrative Agriculture  2014, Vol. 13 Issue (12): 2577-2583    DOI: 10.1016/S2095-3119(14)60755-3
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D
 LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China
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摘要  Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspecific hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene(s) in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, flanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring (CS) nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The flanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.

Abstract  Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspecific hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene(s) in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, flanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring (CS) nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The flanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.
Keywords:  Puccinia striiformis f. sp. tritici       Psathyrostachys huashanica Keng       resistance gene       genetic analysis       SSR markers  
Received: 07 November 2013   Accepted:
Fund: 

The research was supported by the Programme of Introducing Talents of Discipline to Universities, Ministry of Education, China (111 Project, B07049), the National Basic Research Program of China (973 Program, 2013CB127700), the Science and Technology Co-ordinating Innovative Engineering Project of Shaanxi Province, China (2012KTCL02-10), the National Natural Science Foundation of China (30771397), and the China Postdoctoral Science Foundation (2012M512034).

Corresponding Authors:  WANG Bao-tong, Mobile: 13572410050, E-mail: wangbt@nwsuaf.edu.cn; LI Qiang, Mobile: 15319489826, E-mail: qiangli@nwsuaf.edu.cn     E-mail:  wangbt@nwsuaf.edu.cn;qiangli@nwsuaf.edu.cn
About author:  LIU Ze-guang, E-mail: liuzg881111@nwsuaf.edu.cn

Cite this article: 

LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue. 2014. Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D. Journal of Integrative Agriculture, 13(12): 2577-2583.

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