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| Genome characterization of the Caprine arthritis-encephalitis virus in China: A retrospective genomic analysis of the earliest Chinese isolates |
WANG Deng-feng1, 2, YANG Xue-yun2, WEI Yu-rong2, LI Jian-jun2, BOLATI Hongduzi2, MENG Xiao-xiao2, TUERXUN Gunuer2, NUERDAN Nuerbaiheti2, WU Jian-yong2#
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1 Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
2 Xinjiang Key Laboratory of Animal Infectious Diseases, Institute of Veterinary Medicine, Xinjiang Academy of Animal Sciences, Urumqi 830013, P.R.China
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摘要 【目的】山羊关节炎-脑炎病毒（Caprine arthritis-encephalitis virus，CAEV）属于反转录病毒科慢病毒属成员，与梅迪-维斯纳病毒（Maedi visna virus）合称为小反刍动物慢病毒（Small ruminant lentiviruses）。该病毒可感染山羊和绵羊，以奶山羊最为易感。在过去四十年间，CAEV在我国传播扩散，并存在不同程度的感染，但有关该病毒基因组特征和可能的来源研究仍然较少，获取在中国流行的CAEV毒株的基因组数据对于开发诊断方法和根除相关疾病具有重要意义。【方法】本研究使用分段扩增和测序的方法获得了1989–1994年间从我国甘肃、贵州、陕西、山东和四川等地感染奶山羊中分离的5株CAEV病毒基因组，通过基因型分析、全基因组比对、遗传进化和种群结构分析来阐释该病毒基因组特征。【结果】我国CAEV与国外毒株基因组相似度为58%–93%，属于B型小反刍动物慢病毒，可进一步归为B1基因型。遗传进化分析结果显示本研究测定的5株病毒与其它两株中国毒株聚集成簇，亲缘关系最近，同源性为98.3%–99.3%；其次是与美国毒株Clements（GenBank登录号NC_001463.1），相似性为91.8%–92.3%；种群结构分析表明我国已测定的7株病毒拥有与其它已知毒株不同的祖先成分。【结论】跨度5年从我国不同省份分离的CAEV毒株具有高度同源性，分离株可能起源于非已知毒株的其它来源。【创新点】本研究完成了我国最早分离的5株CAEV病毒的基因组测序和基因特征分析，确认我国分离毒株高度同源，可能起源于特定的同一祖先，该结果为研制适用于我国CAEV检测的试剂和CAEV流行病学调查与防控奠定了基础。
Abstract
Caprine arthritis-encephalitis virus (CAEV) is an under-studied virus infecting caprines and ovines worldwide. Over the last four decades, CAEV has spread in China, obtaining genomic data on CAEV strains circulating in China is of importance for developing diagnostic methods and eradicating associated diseases. However, there is limited information on the genome, including characterizations, and the probable origin. This work aimed to characterize Chinese CAEV genomes and population structures. Five CAEV strains isolated from infected dairy goats between 1989 and 1994 in Gansu, Guizhou, Shaanxi, Shandong and Sichuan provinces were cloned and sequenced. The Chinese CAEV had a 58–93% genome similarities to strains outside of China, and they belonged to subgenotype B1. The highest similarity levels (98.3–99.3%) were with two other Chinese strains, and they shared a 91.8–92.3% similarity with the strain Clements (GenBank accession no. NC_001463.1) from outside of China. The Chinese CAEV strains isolated from different provinces over five years were still highly homologous and contained unique ancestral population components, indicating that these Chinese strains had a common origin that differed from other known strains. Our results provide genomic data on circulating Chinese CAEV strains and will be useful for future epidemiological investigations and CAEV eradication programs.
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Received: 07 December 2021
Accepted: 22 May 2022
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| Fund:
This work was funded by the National Key Research and Development Program of China (2016YFD0500908).
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| About author: WANG Deng-feng, E-mail: wangdengfeng713@163.com; #Correspondence WU Jian-yong, Tel: +86-991-3091202, Fax: +86-991-3098109, E-mail: chienyung@foxmail.com |
Cite this article:
WANG Deng-feng, YANG Xue-yun, WEI Yu-rong, LI Jian-jun, BOLATI Hongduzi, MENG Xiao-xiao, TUERXUN Gunuer, NUERDAN Nuerbaiheti, WU Jian-yong.
2023.
Genome characterization of the Caprine arthritis-encephalitis virus in China: A retrospective genomic analysis of the earliest Chinese isolates. Journal of Integrative Agriculture, 22(3): 872-880.
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