|
|
|
Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation |
LIXiao-juan2, GUOCheng-jin1, LUWen-jing2, DUANWei-wei1, ZHAOMiao3, MAChun-ying1, GUJun-tao2, XIAOKai1 |
1、College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China
2、College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China
3、Science & Technology College, North China Electric Power University, Baoding 071051, P.R.China |
|
|
摘要 Zinc finger protein (ZFP) genes comprise a large and diverse gene family, and are involved in biotic and abiotic stress responses in plants. In this study, a total of 126 ZFP genes classified into various types in wheat were characterized and subjected to expression pattern analysis under inorganic phosphate (Pi) deprivation. The wheat ZFP genes and their corresponding GenBank numbers were obtained from the information of a 4×44K wheat gene expression microarray chip. They were confirmed by sequence similarity analysis and named based on their homologs in Brachypodium distachyon or Oriza sativa. Expression analysis based on the microarray chip revealed that these ZFP genes are categorized into 11 classes according to their gene expression patterns in a 24-h of Pi deprivation regime. Among them, ten genes were differentially up-regulated, ten genes differentially down- regulated, and two genes both differentially up- and down-regulated by Pi deprivation. The differentially up- or down-regulated genes exhibited significantly more or less transcripts at one, two, or all of the checking time points (1, 6, and 24 h) of Pi stress in comparison with those of normal growth, respectively. The both differentially up- and down-regulated genes exhibited contrasting expression patterns, of these, TaWRKY70;5 showed significantly up-regulated at 1 and 6 h and down-regulated at 24 h whereas TaAN1AN20-8;2 displayed significantly upregulated at 1 h and downregulated at 6 h under deprivation Pi condition. Real time PCR analysis confirmed the expression patterns of the differentially expressed genes obtained by the microarray chip. Our results indicate that numerous ZFP genes in wheat respond to Pi deprivation and have provided further insight into the molecular basis that plants respond to Pi deprivation mediated by the ZFP gene family.
Abstract Zinc finger protein (ZFP) genes comprise a large and diverse gene family, and are involved in biotic and abiotic stress responses in plants. In this study, a total of 126 ZFP genes classified into various types in wheat were characterized and subjected to expression pattern analysis under inorganic phosphate (Pi) deprivation. The wheat ZFP genes and their corresponding GenBank numbers were obtained from the information of a 4×44K wheat gene expression microarray chip. They were confirmed by sequence similarity analysis and named based on their homologs in Brachypodium distachyon or Oriza sativa. Expression analysis based on the microarray chip revealed that these ZFP genes are categorized into 11 classes according to their gene expression patterns in a 24-h of Pi deprivation regime. Among them, ten genes were differentially up-regulated, ten genes differentially down- regulated, and two genes both differentially up- and down-regulated by Pi deprivation. The differentially up- or down-regulated genes exhibited significantly more or less transcripts at one, two, or all of the checking time points (1, 6, and 24 h) of Pi stress in comparison with those of normal growth, respectively. The both differentially up- and down-regulated genes exhibited contrasting expression patterns, of these, TaWRKY70;5 showed significantly up-regulated at 1 and 6 h and down-regulated at 24 h whereas TaAN1AN20-8;2 displayed significantly upregulated at 1 h and downregulated at 6 h under deprivation Pi condition. Real time PCR analysis confirmed the expression patterns of the differentially expressed genes obtained by the microarray chip. Our results indicate that numerous ZFP genes in wheat respond to Pi deprivation and have provided further insight into the molecular basis that plants respond to Pi deprivation mediated by the ZFP gene family.
|
Received: 21 October 2013
Accepted:
|
Fund: This work was supported by the National Natural Science Foundation of China (31201674 and 31371618), the Natural Science Foundation of Hebei Province, China (C2011204031) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China. |
Corresponding Authors:
XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn
E-mail: xiaokai@hebau.edu.cn
|
Cite this article:
LIXiao-juan2 , GUOCheng-jin1 , LUWen-jing2 , DUANWei-wei1 , ZHAOMiao3 , MAChun-ying1 , GUJun-tao2 , XIAOKai1 .
2014.
Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation. Journal of Integrative Agriculture, 13(8): 1621-1633.
|
Baldwin J C, Karthikeyan A S, Raghothama K G. 2002. LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato. Plant Physiology, 125, 728-737 Bariola P A, Howard C J, Taylor C B, Verburg M T, Jaglan V D, Green P J. 1994. The Arabidopsis ribonuclease gene RNS1 is tightly controlled in response to phosphate limitation. The Plant Journal, 6, 673-685 Bartels D, Sunkar R. 2005. Drought and salt tolerance in plants. Critical Review in Plant Sciences, 24, 23-58 Berg J M, Shi Y. 1996. The galvanization of biology: a growing appreciation for the roles of zinc. Science, 271, 1081-1085 Boocock G R B, Marit M R, Rommens J M. 2006. Phylogeny, sequence conservation, and functional complementation of the SBDS protein family. Genomics, 87, 758-771 Chebud Y, Naja G M, Rivero R. 2011. Phosphorus run-off assessment in a watershed. Journal of Environment Monitoring, 13, 66-73 Ciftci-Yilmaz S, Mittler R. 2008. The zinc finger network of plants. Cellular and Molecular Life Sciences, 65, 1150- 1160. Cui J, Jander G, Racki L R, Kim P D, Pierce N E, Ausubel F M. 2002. Signals involved in Arabidopsis resistance to Trichoplusiani caterpillars induced by virulent and avirulent strains of the phytopathogen Pseudomonas syringae. Plant Physiology, 129, 551-564 Devaiah B N, Karthikeyan A S, Raghothama K G. 2007a. WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiology, 143, 1789-1801 Devaiah B N, Nagarajna V K, Raghothama K G. 2007b. Phosphate homeostasis and root development in Arabidopsis are synchronized by the zing finger transcription factor ZAT6. Plant Physiology, 145, 147-159 Emerson R O, Thomas J H. 2009. Adaptive evolution in zinc finger transcription factors. PLoS Genetics, 5, e1000325. Englbrecht C C, Schoof H, Böhm S. 2004. Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome. BMC Genomics, 5, 39. Feurtado J A, Huang D, Wicki-Stordeur L, Hemstock L E, Potentier M S. 2011. The Arabidopsis C2H2 zinc finger INDETERMINATE DOMAIN1/EMJUDRPIS promotes the transition to germination by regulating light and hormonal signaling during seed maturation. The Plant Cell, 23, 1772-1794 Fowler S, Thomashow M F. 2002. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. The Plant Cell, 14, 1675-1690 Giri J, Vij S, Dansana P K, Tyagi A K. 2011. Rice A20/ AN1 zinc-finger containing stress-associated proteins (SAP1/11) and a receptor-like cytoplasmic kinase (OsRLCK253) interact via A20 zinc-finger and confer abiotic stress tolerance in transgenic Arabidopsis plants. New Phytologist, 191, 721-732 Gourcilleau D, Lenne C, Armenise C, Moulia B, Julien J. 2011. Phylogenetic study of plant Q-type C2H2 zinc finger proteins and expression analysis of poplar genes in response to osmotic, cold and mechanical stresses. DNA Research, 18, 77-92 Guo C J, Zhao X L, Liu X M, Zhang L J, Gu J T, Li X J, Lu W J, Xiao K. 2013. Function of wheat phosphate transporter gene TaPHT2;1 in Pi translocation and plant growth regulation under replete and limited Pi supply conditions. Planta, 237, 1163-1178 Hall T M. 2005. Multiple modes of RNA recognition by zinc finger proteins. Current Opinion in Structural Biology, 15, 367-373 Hammond J P, Bennett M J, Bowen H C, Broadley M R, Eastwood D C, May S T, Rahn C, Swarup R, Woolaway K E, White P J. 2003. Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants. Plant Physiology, 132, 578-596 Howarth R, Sharpley A, Walker D. 2002. Sources of nutrient pollution to coastal waters in the United States: Implications for achieving coastal water quality goals. Estuaries and Coasts, 25, 656-676 Kilian J, Peschke F, Berendzen K W, Harter K, Wanke D. 2012. Prerequisites, performance and profits of transcriptional profiling the abiotic stress response. Biochimica Biophysica Acta, 1819, 166-175 Kosarev P, Mayer K F, Hardtke C S. 2002. Evaluation and classification of RING-finger domains encoded by the Arabidopsis genome. Genome Biology, 3, RESEARCH0016. Laity J H, Lee B M, Wright P E. 2001. Zinc finger proteins: new insights into structural and functional diversity. Currrent Opinion in Structural Biology, 11, 39-46 Lijavetzky D, Carbonero P, Vicente-Carbajosa J. 2003. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families. BMC Evolutional Biology, 3, 17. Liu X M, Zhao X L, Zhang L J, Lu W J, Li X J, Xiao K. 2013. TaPht1;4, a high-affinity phosphate transporter gene in wheat (Triticum aestivum L.), plays an important role in plant phosphate acquisition under phosphorus deprivation. Functional Plant Biology, 40, 329-341 Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25, 402-408 Ma W, Ma L, Li J, Wang F, Sisak I, Zhang F. 2011. Phosphorus flows and use efficiencies in production and consumption of wheat, rice, and maize in China. Chemosphere, 84, 814-821 MacDonald G K, Bennett E M, Potter P A, Ramankutty N. 2011. Agronomic phosphorus imbalances across the world’s croplands. Proceedings of the National Academy Sciences of the United States of America, 108, 3086-3091 Mackay J P, Crossley M. 1998. Zinc fingers are sticking together. Trends in Biochemical Sciences, 23, 1-4 Misson J, Raghothama K G, Jain A, Jouhet J, Block M A, Bligny R, Ortet P, Creff A, Somerville S, Rolland N. 2005. A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proceedings of the National Academy Sciencesof the United States of America, 102, 11934-11939 Moore M, Ullman C. 2003. Recent developments in the engineering of zinc finger proteins. Brief Funct Genomic Proteomic, 1, 342-355 Moss B. 2008. Water pollution by agriculture. Philosophical Transactions of the Royal Society of London (B: Biological Sciences), 363, 659-666 Mudge S R, Rae A L, Diatloff E, Smith F W. 2002. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. The Plant Journal, 31, 341-353 Mukhopadhyay A, Vij S, Tyagi A K. 2004. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proceedings of the National Academy Sciences of the United States of America, 101, 6309-6314 Nakano T, Suzuki K, Fujimura T, Shinshi H. 2006. Genome- wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology, 140, 411-432 Rabbani M A, Maruyama K, Abe H, Khan M A, Katsura K, Ito Y, Yoshiwara K, Seki M, Shinozaki K, Yamaguchi- Shinozaki K. 2003. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiology, 133, 1755-1767 Raghothama K G. 1999. Phosphate acquisition. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 665-693 Ronald P, Leung H. 2002. The rice genome: The most precious things are not jade and pearls. Science, 296, 58-59 Rubio V, Francisco L, Roberto S, Ana C, Martin J I, Antonio L, Paz-Ares J. 2001. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes & Development, 15, 2122-2133 Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K. 2004. Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiology, 136, 2734-2746 Schachtman D P, Reid R J, Ayling S. 1998. Phosphorus uptake by plants: from soil to cell. Plant Physiology, 116, 447-453 Schumann U, Prestele J, O’Geen H, Brueggeman R, Wanner G, Gietl C. 2007. Requirement of the C3HC4 zinc RING finger of the Arabidopsis PEX10 for photorespiration and leaf peroxisome contact with chloroplasts. Proceedings of the National Academy Sciences of the United States of America, 104, 1069-1074 Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T. 2002. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. The Plant Journal, 31, 279-292 Sun Z H, Ding C H, Li X J, Xiao K. 2012. Molecular characterization and expression analysis of TaZFP15, a C2H2-type zinc finger transcription factor gene in wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 11, 31-42 IBI (The International Brachypodium Initiative). 2010. Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature, 463, 763-768 Uhde-Stone C, Zinn K E, Ramirez-Yanez M, Li A, Vance C P, Allan D L. 2003. Nylon filter arrays reveal differential gene expression in proteoid roots of white lupin in response to phosphorus deficiency. Plant Physiology, 131, 1064-1079 Vance C P, Uhde-Stone C, Allan D L. 2003. Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytologist, 157, 423-447 Woo J, MacPherson C R, Liu J, Wang H, Kiba T, Hannah M A, Wang X J, Bajic V B, Chua N H. 2012. The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation. BMC Plant Biology, 12, 62. Wu P, Ma L, Hou X, Wang M, Wu Y, Liu F, Deng X W. 2003. Phosphate starvation triggers distinct alterations of genome expression in Arabidopsis roots and leaves. Plant Physiology, 132, 1260-1271 Yamaguchi-Shinozaki K, Shinozaki K. 2006. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology, 57, 781-803 Zhang Y, Wang L. 2005. The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evolutional Biology, 5, 1. |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|