Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure
KE Xin, WANG Chun-yong, LI Run-dong , ZHANG Yun
1、College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, P.R.China
2、College of Land and Environment, Shenyang Agriculture University, Shenyang 110161, P.R.China
摘要 The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37°C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L-1, respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.
Abstract The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37°C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L-1, respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.
KE Xin, WANG Chun-yong, LI Run-dong , ZHANG Yun.
2014.
Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure. Journal of Integrative Agriculture, 13(6): 1373-1381.
Álvarez J A, Otero L, Lema J M, Omil F. 2010. The effectand fate of antibiotics during the anaerobic digestion ofpig manure. Bioresource Technology, 101, 8581-8586
APHA. 1995. Standard Methods for the Examination ofWater and Wastewater. 19th ed. American Public HealthAssociation, Washington, D. C.
Arikan O A, Sikora L J, Mulbry W, Khan S U, Rice C, FosterG D. 2006. The fate and effect of oxytetracycline during theanaerobic digestion of manure from therapeutically treatedcalves. Process Biochemistry, 41, 1637-1643
Asakawa S, Nagaoka K. 2003. Methanoculleus bourgensis,Methanoculleus olentangyi and Methanoculleusoldenburgensis are subjective synonyms. InternationalJournal of Systematic and Evolutionary Microbiology,53, 1551-1552
Ben W W, Qiang Z M, Adams C, Zhang H Q, Chen L P. 2008.Simultaneous determination of sulfonamides, tetracyclinesand tiamulin in swine wastewater by solid-phase extractionand liquid chromatography-mass spectrometry. Journal ofChromatography (A), 1202, 173-180
Beneragama N, Lateef S A, Iwasaki M, Yamashiro T,Umetsu K. 2013. The combined effect of cefazolinand oxytertracycline on biogas production fromthermophilic anaerobic digestion of dairy manure.Bioresource Technology, 133, 23-30
Bertolini J M, Wakabayashi H, Watral V G, Whipple M J,Rohovec J S. 1994. Electrophoretic detection of proteasesfrom selected strains of Flexibacter psychrophilus andassessment of their variability. Journal of Aquatic AnimalHealth, 6, 224-233
Chachkhiani M, Dabert P, Abzianidze T, Partskhaladze G,Tsiklauri L, Dudauri T, Godon J J. 2004. 16S rDNAcharacterisation of bacterial and archaeal communitiesduring start-up of anaerobic thermophilic digestion of cattlemanure. Bioresource Technology, 93, 227-232
Chee-Sanford J C, Aminov R I, Krapac I J, Garrigues-Jeanjean N, Mackie R I. 2001. Occurrence and diversity oftetracycline resistance genes in lagoons and groundwaterunderlying two swine production facilities. Applied andEnvironment Microbiology, 67, 1494-1502
Dröge S, Fröhlich J, Radek R, König H. 2006. Spirochaetacoccoides sp. nov., a novel coccoid spirochete from thehindgut of the termite neotermes castaneus. Applied andEnvironment Microbiology, 72, 392-397
Gamel-El-Din H. 1986. Biogas production from antibioticcontaminated cow manure. In: Proceedings of theInternational Conference on Biogas Technology, TransferDiffusion. Springer, Netherlands. pp. 480-487
Green D H, Llewellyn L E, Negri A P, Blackburn S I, BolchC J. 2004. Phylogenetic and functional diversity ofthe cultivable bacterial community associated with theparalytic shellfish poisoning dinoflagellate Gymnodiniumcatenatum. FEMS Microbiology Ecology, 47, 345-357
Hashizume T, Takai C, Naito M, Morisaki H. 2005.Characteristics of the mucus layer on the surface of thebluegill (Lepomis macrochirus) and the bacterial flora inthe mucus. Microbes and Environments, 20, 69-80
Higashioka Y, Kojima H, Nakagawa T, Sato S, Fukui M. 2009.A novel n-alkane-degrading bacterium as a minor memberof p-xylene-degrading sulfate-reducing consortium.Biodegradation, 20, 383-390
Holm-Hielsen J B, Alseadi T, Oleskowicz-Popiel P. 2009.The future of anaerobic digestion and biogas utilization.Bioresource Technology, 100, 5478-5484
Janssen P H, Kirs M. 2008. Structure of the archaeal communityin the rumen. Applied and Environment Microbiology, 74,3619-3625
Kamiński M, Kartanowicz R, Jastrzebski D, Kamiński M M.2003. Determination of carbon monoxide, methane andcarbon dioxide in refinery hydrogen gases and air by gaschromatography. Journal of Chromatography (A), 989,277-283
Konishi I, Hoshino T, Kondo Y, Saito K, Nishiguchi M, SatoK, Fujiwara T. 2009. Phylogenetic analyses and detectionof viridans streptococci based on sequences and denaturinggradient gel electrophoresis of the rod shape-determiningprotein gene. Journal of Oral Microbiology, doi:10.3402/jom.v1i0.2015
Kotzerke A, Hammesfahr U, Kleineidam K, Lamshoft M,Thiele-Bruhn S, Schloter M, Wilke B M. 2011. Influence ofdifloxacin-contaminated manure on microbial communitystructure and function in soils. Biology and Fertility ofSoils, 47, 177-186
Krakat N, Schmidt S, Scherer P. 2011. Potential impactof process parameters upon the bacterial diversityin the mesophilic anaerobic digestion of beet silage.Bioresource Technology, 102, 5692-5701
Lallai A, Mura G, Onnis N. 2002. The effects of certainantibiotics on biogas production in the anaerobic digestionof pig waste slurry. Bioresource Technology, 82, 205-208
Lee J H, Rhee M S, Kumar S, Lee G H, Chang D H, KimD S, Choi S H, Lee D W, Yoon M H, Kima B C. 2013.Genome sequence of methanobrevibacter sp. strain JH1,isolated from rumen of korean native cattle. GenomeAnnouncements, 1, e00002-e00013.
Leschine S, Paster B J, Canale-Parola E. 2006. Free-livingsaccharolytic spirochetes: The genus Spirochaeta. In: The Prokaryotes. Springer New York. pp. 195-210
Luo Y, Mao D, Rysz M, Zhou Q, Zhang H, Xu L, Alvarez P JJ. 2010. Trends in antibiotic resistance genes occurrencein the Haihe River, China. Environmental Science &Technology, 44, 7220-7225
Macauley J J, Adams C D, Mormile M R. 2007. Diversityof tetresistance genes in tetracycline-resistant bacteriaisolated from a swine lagoon with low antibiotic impact.Canadian Journal of Microbiology, 53, 1307-1315
Massé D I, Lu D, Masse L, Droste R L. 2000. Effect ofantibiotics on psychrophilic anaerobic digestion ofswine manure slurry in sequencing batch reactors.Bioresource Technology, 75, 205-211
Mikucki J A, Liu Y, Delwiche M, Colwell F S, Boone DR. 2003. Isolation of a methanogen from deep marinesediments that contain methane hydrates, and descriptionof Methanoculleus submarinus sp. nov. Applied andEnvironment Microbiology, 69, 3311-3316.
Sarmah A K, Meyer M T, Boxall A. 2006. A global perspectiveon the use, sales, exposure pathways, occurrence, fate andeffects of veterinary antibiotics (VAs) in the environment.Chemosphere, 65, 725-759.
Supaphol S, Jenkins S N, Intomo P, Waite I S, O’DonnellA G. 2011. Microbial community dynamics inmesophilic anaerobic co-digestion of mixed waste.Bioresource Technology, 102, 4021-4027.
Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4:Molecular evolutionary genetics analysis (MEGA)software version 4.0. Molecular Biology and Evolution,24, 1596-1599.
Trzcinski A P, Ray M J, Stuckey D C. 2010. Performance ofa three-stage membrane bioprocess treating the organicfraction of municipal solid waste and evolution of itsarchaeal and bacterial ecology. Bioresource Technology,101, 1652-1661.
Tu W, Zhang L X, Zhou Z, Liu X, Fu Z. 2011. The developmentof renewable energy in resource-rich region: A case inChina. Renewable and Sustainable Energy Reviews, 15,856-860.
Wang H, Lehtomäki A, Tolvanen K, Puhakka J, Rintala J.2009. Impact of crop species on bacterial communitystructure during anaerobic co-digestion of crops and cowmanure. Bioresource Technology, 100, 2311-2315.
Wang H, Tolvanen K, Lehtomäki A, Puhakka J, Rintala J.2010. Microbial community structure in anaerobic codigestionof grass silage and cow manure in a laboratorycontinuously stirred tank reactor. Biodegradation, 21,135-146.
Xia S, Jia R, Feng F, Xie K, Li H, Jing D, Xu X. 2012. Effectof solids retention time on antibiotics removal performanceand microbial communities in an A/O-MBR process.Bioresource Technology, 106, 36-43.
Yoon J H, Park S, Jung Y T. 2013. Mucilaginibactercalamicampi s p . n o v . , a member of t h efamily Sphingobacteriaceae isolated from soil at a fieldof reeds. Antonie van Leeuwenhoek, 104, 37-45.
Yoshikawa S, Yasokawa D, Nagashima K, Yamazaki K,Kurihara H, Ohta T, Kawai Y. 2010. Microbiota duringfermentation of chum salmon (Oncorhynchus keta) saucemash inoculated with halotolerant microbial starters:Analyses using the plate count method and PCR-denaturinggradient gel electrophoresis (DGGE). Food Microbiology,27, 509-514.
