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| Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development |
| SUN Jian-lei, SUI Xiao-lei, HUANG Hong-yu, WANG Shao-hui, WEI Yu-xia , ZHANG Zhenxian |
1、Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops/College of Agriculture and Biotechnology,
China Agricultural University, Beijing 100193, P.R.China
2、Plant Science Department, Beijing University of Agriculture, Beijing 102206, P.R.China |
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摘要 Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber (Cucumis sativus L.) in solar greenhouses in northern China. Here, two genotypes of cucumber (Deltastar and Jinyan 2) are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate (PN), stomatal conductance (gs), Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency (ФPSII) of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching (NPQ). Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m-2 s-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m-2 s-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.
Abstract Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber (Cucumis sativus L.) in solar greenhouses in northern China. Here, two genotypes of cucumber (Deltastar and Jinyan 2) are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate (PN), stomatal conductance (gs), Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency (ФPSII) of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching (NPQ). Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m-2 s-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m-2 s-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.
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Received: 28 February 2013
Accepted:
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| Fund: This work was supported by China Agriculture Research System (CARS-25), the National Basic Research Program of China (973 Program, 2009CB11900) and the National Key Technologies R&D Program of China (2011BAD12B01). |
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Corresponding Authors:
ZHANG Zhen-xian, Tel: +86-10-62731952, Fax: +86-10-62731952, E-mail: zhangzx@cau.edu.cn
E-mail: zhangzx@cau.edu.cn
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| About author: SUN Jian-lei, E-mail: sunjianlei06@hotmail.com; SUI Xiao-lei, E-mail: sui-office@cau.edu.cn |
Cite this article:
SUN Jian-lei, SUI Xiao-lei, HUANG Hong-yu, WANG Shao-hui, WEI Yu-xia , ZHANG Zhenxian.
2014.
Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development. Journal of Integrative Agriculture, 13(5): 997-1007.
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| Ananieva K, Ananiev E D, Mishev K, Georgieva K, Tzvetkova N, van Staden J. 2008. Changes in photosynthetic capacity and polypeptide patterns during natural senescence and rejuvenation of Cucurbita pepo L. (zucchini) cotyledons. Plant Growth Regulation, 54, 23- 29. Bate N J, Rothstein S J, Thompson J E. 1991. Expression of nuclear and chloroplast photosynthesis-specific genes during leaf senescence. Journal of Experimental Biology, 42, 801-811 Bilger W, Bjorkman O. 1990. Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbency changes, fluorescence and photosynthesis in leaves of hedera-canariensis. Photosynthetic Research, 25, 173-185 Bradford M M. 1976. New, rapid, sensitive method for protein determination. Federation Proceedings, 35, 274- 274. Brouwer B, Ziolkowska A, Bagard M, Keech O, Gardestrom P. 2012. The impact of light intensity on shade-induced leaf senescence. Plant Cell Environment, 35, 1084-1098 Butz N D, Sharkey T D. 1989. Activity ratios of ribulose- 1,5-bisphosphate carboxylase accurately reflect carbamylation ratios. Plant Physiology, 89, 735-739 Cen Y P, Sage R F.2005. The regulation of Rubisco activity in response tovariation in temperature and atmospheric CO2 partial pressure in sweet potato. Plant Physiology, 139, 979-990 Craftsbrandner S J. 1992. Phosphorus-nutrition influence on leaf senescence in soybean. Plant Physiology, 98, 1128- 1132. Dean C, Pichersky E, Dunsmuir P. 1989. Dunsmuir, Structure, evolution, and regulation of Rbcs genes in higher-plants. Annual Review of Plant Physiology and Molecule Biology, 40, 415-439 Evans P T, Malmberg R L. 1989. Do polyamines have roles in plant development. Annual Review of Plant Physiology and Molecule Biology, 40, 235-269 Ford D M, Shibles R. 1988. Photosynthesis and other traits in relation to chloroplast number during soybean leaf senescence. Plant Physiology, 86, 108-111 Gao L H, Qu M, Ren H Z, Sui X L, Chen Q Y, Zhang Z X. 2010. Structure, function, application, and ecological benefit of a single-slope, energy-efficient solar greenhouse in China. HortTechnology, 20, 626-631 Genty B, Briantais J M, Baker N R. 1989. The relationship between the quantum yield of photosynthetic electron- transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta, 990, 87-92 Gepstein S. 1998. Photosynthesis. In: Noodén L D, Leopold A C, eds., Senescence and Aging in Plants. Academic Press, San Diego, CA. pp. 85-109 Guo Y, Cai Z, Gan S. 2004. Transcriptome of Arabidopsis leaf senescence. Plant Cell Environmet, 27, 521-549 He Z L, Caemmerer V S, Hudson G S, Price G D, Badger M R, Andrews T J. 1997. Ribulose-1,5-bisphosphate carboxylase/oxygenase activase deficiency delays senescence of ribulose-1,5-bisphosphate carboxylase/ oxygenase but progressively impairs its catalysis during tobacco leaf development Plant Physiology, 115, 1569- 1580. Hudson G S, Evans J R, Voncaemmerer S, Arvidsson Y B C, Andrews T J. 1992. Reduction of ribulose- 1,5-bisphosphate carboxylase oxygenase content by antisense RNA Reduces photosynthesis in transgenic tobacco plants. Plant Physiology, 98, 294-302 Imai K, Suzuki Y, Mae T, Makino A. 2008. Changes in the synthesis of Rubisco in rice leaves in relation to senescence and N influx. Annals of Botany, 101, 135- 144. Imai K, Suzuki Y, Makino A, Mae T. 2005. Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose- 1,5-bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence. Plant Cell Environment, 28, 1589-1600 J i a n g C Z, Rodermel S R, Shibles R M 1993. Photosynthesis, Rubisco activity and amount, and their regulation by transcription in senescing soybean leaves. Plant Physiology, 101, 105-112. J iang C Z, Rodermel S R. 1995. Regulation of photosynthesis during leaf development in Rbcs antisense DNA mutants of tobacco. Plant Physiology, 107, 215-224 Li W, Huang J L, Sui X L, Wang S H, Guan Q Z, Zhou M, Hu L P, Zhang Z X. 2008. Effects of low light on photosynthetic and fluorescent characteristics of seedlings of cucumis sativus. Acta Horticulturae Sinica, 35, 119-122 (in Chinese) Lu C M, Zhang J H. 1998. Changes in photosystem II function during senescence of wheat leaves. Physiologia Plantarum, 104, 239-247 M a k i n o A, Mae T, Ohira K. 1984. Changes in photosynthetic capacity in rice leaves from emergence through senescence - Analysis from ribulose-1,5- bisphosphate carboxylase and leaf conductance Plant Cell Physiology, 25, 511-521. Makino A, Sakashita H, Hidema J, Mae T, Ojima K, Osmond B. 1992. Distinctive responses of ribulose- 1,5-bisphosphate carboxylase and carbonic anhydrase in wheat Leaves to nitrogen nutrition and their possible relationships to CO2-transfer resistance. Plant Physiology, 100, 1737-1743 Miller A, Schlagnhaufer C, Spalding M, Rodermel S. 2000. Carbohydrate regulation of leaf development: Prolongation of leaf senescence in Rubisco antisense mutants of tobacco. Photosynthetic Research, 63, 1-8 Murchie E H, Hubbart S, Peng S, Horton P. 2005. Acclimation of photosynthesis to high irradiance in rice: gene expression and interactions with leaf development. Journal of Experimental Botany,56, 449-460 Nikolau B J, Klessig D F. 1987. Coordinate, organ- specific and developmental regulation of ribulose 1,5-bisphosphate carboxylase gene-expression in amaranthus-hypochondriacus. Plant Physiology, 85, 167-173 Pandey S K, Singh H. 2011. A simple, cost-effective method for leaf area estimation. Journal of Bontany, doi:10.1155/2011/658240. Quick W P, Schurr U, Fichtner K, Schulze E D, Rodermel S R, Bogorad L, Stitt M. 1991. The impact of decreased Rubisco on photosynthesis, growth, allocation and storage in tobacco plants which have been transformed with antisense Rbcs. The Plant Journal, 1, 51-58 Rodermel S, Haley J, Jiang C Z, Tsai C H, Bogorad L. 1996. A mechanism for intergenomic integration: Abundance of ribulose bisphosphate carboxylase small-subunit protein influences the translation of the large- subunit mRNA. Proceedings of the National Academy of Sciences of the United States of America, 93, 3881-3885 Secor J, Shibles R, Stewart C R. 1984. A metabolic comparison between progressive and monocarpic senescence of soybean. Canadian Journal of Botnay, 62, 806-811 Smart C M. 1994. Gene-expression during leaf senescence. New Phytologist, 126, 419-448 Sui X L, Mao S L, Wang L H, Zhang B X, Zhang Z X. 2012. Effect of low light on the characteristics of photosynthesis and chlorophyll a fluorescence during leaf development of sweet pepper. Journal of Integrative Agriculture, 11, 1633-1643 Sui X L, Sun J L, Wang S H, Li W, Hu L P, Meng F Z, Fan Y Y, Zhang Z X. 2011. Photosynthetic induction in leaves of two cucumber genotypes differing in sensitivity to low-light stress. African Journal of Biotechnology, 10, 2238-2247 Suzuki Y, Makino A, Mae T. 2001. Changes in the turnover of Rubisco and levels of mRNAs of rbcL and rbcS in rice leaves from emergence to senescence. Plant Cell Environment, 24, 1353-1360 Suzuki Y, Miyamoto T, Yoshizawa R, Mae T, Makino A. 2009a. Rubisco content and photosynthesis of leaves at different positions in transgenic rice with an over expression of RBCS. Plant Cell Environment, 32, 417- 427. Suzuki Y, Nakabayashi K, Yoshizawa R, Mae T, Makino A. 2009b. Differences in expression of the RBCS multigene family and Rubisco protein content in various rice plant tissues at different growth stages. Plant Cell Physiology, 50, 1851-1855 Suzuki Y, Ohkubo M, Hatakeyama H, Ohashi K, Yoshizawa R, Kojima S, Hayakawa T, Yamaya T, Mae T, Makino A. 2007. Increased Rubisco content in transgenic rice transformed with the “sense” rbcS gene. Plant Cell Physiology, 48, 626-637 Tholen D, Pons T L, Voesenek L A C J, Poorter H. 2007. Ethylene insensitivity results in down-regulation of Rubisco expression and photosynthetic capacity in tobacco. Plant Physiology, 144, 1305-1315 Weng X Y, Xu H X, Jiang D A. 2005. Characteristics of gas exchange, chlorophyll fluorescence and expression of key enzymes in photosynthesis during leaf senescence in rice plant. Journal of Integrative Plant Biology, 47, 560- 566. Wingler A, Schaewen V A, Leegood R C, Lea P J, Quick W P. 1998. Quick, Regulation of leaf senescence by cytokinin, sugars, and light - Effects on NADH- dependent hydroxypyruvate reductase. Plant Physiology, 116, 329-335 Winterma J f, Demots A. 1965. Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochimica et Biophysica Acta, 109, 448-453 Yoo S D, Greer D H, Laing W A, McManus M T. 2003. Changes in photosynthetic efficiency and carotenoid composition in leaves of white clover at different developmental stages. Plant Physiology and Biochemistry, 41, 887-893 Zhang N, Schurmann P, Portis A R. 2001.Characterization of the regulatory function of the 46-kDa isoform of Rubisco activase from Arabidopsis. Photosynthetic Research, 68, 29-37 Zheng B S. 2006. The Research Technology of Modern Plant Physiology and Biochemistry. China Meteorological Press, Beijing. (in Chinese) Zhou Y H, Yu J Q, Huang L F, Nogues S. 2004. The relationship between CO2 assimilation, photosynthetic electron transport and water-water cycle in chill-exposed cucumber leaves under low light and subsequent recovery. Plant Cell Environment, 27, 1503-1514 |
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