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Journal of Integrative Agriculture  2021, Vol. 20 Issue (11): 3060-3074    DOI: 10.1016/S2095-3119(20)63485-2
Special Issue: 食品科学合辑Food Science
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Melatonin treatment induces chilling tolerance by regulating the contents of polyamine, γ-aminobutyric acid, and proline in cucumber fruit
Miilion P MADEBO, LUO Si-ming, WANG Li, ZHENG Yong-hua, JIN Peng
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China
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以商业成熟期黄瓜果实为材料,研究褪黑素(melatonin,MT)诱导黄瓜果实耐冷性的机制。本研究中,施用100 µmol L-1 MT处理黄瓜果实,然后在4°C、90%相对湿度条件下贮藏15 d。与对照相比,MT处理减轻了黄瓜的冷害,电解质泄漏减少,硬度增强。在贮藏期间MT处理抑制了黄瓜果实中叶绿素酶的活性,提高了叶绿素的含量。MT处理增强了精氨酸脱羧酶(arginine decarboxylase,ADC)和鸟氨酸脱羧酶(ornithine decarboxylase,ODC)的活性,而且上调了CsADC (Cucumis sativus ADC)和CsODC (C. sativus ODC) 基因的表达,导致多胺含量的积累。类似的,处理后的黄瓜果实含有较高的脯氨酸水平。与此同时脯氨酸合成酶△1-吡咯啉-5-羧酸合成酶(△1-pyrroline-5-carboxylate synthetase,P5CS)和鸟氨酸转氨酶(ornithine aminotransferase,OAT)的活性显著提高,而脯氨酸降解酶脯氨酸脱氢酶(proline dehydrogenase,PDH)的活性受到抑制。MT也诱导了CsOAT (C. sativus OAT)和CsP5CS (C. sativus P5CS)基因的表达。此外,MT处理通过增强黄瓜果实中GABA转氨酶(GABA transaminase,GABA-T)和谷氨酸脱羧酶(glutamate decarboxylase,GAD)的活性,上调了CsGAD(C. sativus GAD)基因的表达,从而提高γ-氨基丁酸(γ-aminobutyric acid,GABA)的含量。综上所述,结果表明MT处理提高耐冷性,与调节多胺、脯氨酸和γ-氨基丁酸有关。

The mechanism of melatonin (MT) induced chilling tolerance in harvested cucumber fruit was investigated at commercial maturity.  In this study, cucumber fruits were treated with 100 μmol L–1 MT at 4°C and 90% relative humidity for 15 d of storage.  In comparison with the control, cucumber treatment with MT resulted in reduced chilling injury (CI), decreased electrolyte leakage and enhanced firmness.  The fruits treated with MT showed higher chlorophyll contents in storage conditions with suppressed chlorophyllase enzyme activity.  MT treatment increased arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) enzyme activities.  Moreover, enhanced expression of the Cucumis sativus ADC (CsADC) and C. sativus ODC (CsODC) genes resulted in the accumulation of polyamine contents.  Similarly, proline levels exhibited higher levels among treated fruits.  Meanwhile, the proline synthesizing enzymes △1-pyrroline-5-carboxylate syntheses (P5CS) and ornithine aminotransferase (OAT) were significantly increased, while a catabolic enzyme of proline dehydrogenase (PDH) activity was inhibited by treatment.  In addition, MT induced expression of C. sativus OAT (CsOAT) and C. sativus P5CS (CsP5CS) genes.  Cucumber fruits treated with MT also exhibited higher γ-aminobutyric acid (GABA) content by enhanced GABA transaminase (GABA-T) and glutamate decarboxylase (GAD) enzyme activities and a higher C. sativus GAD (CsGAD) gene expression.  To sum up, the results show that MT treatment enhanced chilling tolerance, which was associated with the regulation of polyamines, as well as proline and γ-aminobutyric acid.
Keywords:  cucumber        melatonin        polyamine        proline        GABA        chilling injury  
Received: 06 May 2020   Accepted:
Fund: This study was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Program for Student Innovation through Research and Training (SRT), China (1918C12).
Corresponding Authors:  Correspondence JIN Peng, Tel: +86-25-84395315, Fax: +86-25-84395618, E-mail:   
About author:  Miilion P MADEBO,E-mail:;

Cite this article: 

Miilion P MADEBO, LUO Si-ming, WANG Li, ZHENG Yong-hua, JIN Peng. 2021. Melatonin treatment induces chilling tolerance by regulating the contents of polyamine, γ-aminobutyric acid, and proline in cucumber fruit. Journal of Integrative Agriculture, 20(11): 3060-3074.

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