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Journal of Integrative Agriculture  2023, Vol. 22 Issue (6): 1618-1630    DOI: 10.1016/j.jia.2022.10.014
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Variations in chlorophyll content, stomatal conductance and photosynthesis in Setaria EMS mutants

TANG Chan-juan*, LUO Ming-zhao*, ZHANG Shuo, JIA Guan-qing, TANG Sha, JIA Yan-chao, ZHI Hui, DIAO Xian-min#

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
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摘要  

叶绿素(Chl)含量,尤其是Chl b含量和气孔导度(Gs)是影响净光合速率(Pn)的关键因素。谷子(Setaria italica)是二倍体C4禾本科植物,基因组简单,转化效率高,已被广泛应用于光合作用和耐旱研究中。本研究对48EMS诱变的谷子材料进行了Chl含量、Gs以及Pn的表征。发现分别有2434以及35个突变体材料在Chl含量、Gs以及Pn上发生了显著的变异。相关分析表明,Gs的增加与Pn的增加存在显著正相关关系,Chl b含量的降低与Pn的降低存在弱相关关系。值得注意的是,我们鉴定到两个突变体,Chl b含量显著低于对照豫谷1号,但Pn显著高于豫谷1号。同时,有7个突变体,Gs显著降低于对照,但Pn不显著降低。由此,本研究鉴定了丰富的遗传变异株系,对这些株系对进一步研究将有助于解析Chl含量、GsPn之间的关系,以及C4物种高效光合和节水的机理



Abstract  

Chlorophyll (Chl) content, especially Chl b content, and stomatal conductance (Gs) are key factors that greatly affect net photosynthetic rate (Pn).  Setaria italica, a diploid C4 panicoid species with a simple genome and high transformation efficiency, has been widely accepted as a model in photosynthesis and drought-tolerance research.  In the current study, Chl content, Gs, and Pn of 48 Setaria mutants induced by ethyl methanesulfonate were characterized.  A total of 24, 34 and 35 mutants had significant variations in Chl content, Gs, and Pn, respectively. Correlation analysis showed that positive correlation exists between increased Gs and increased Pn, and a weak correlation between decreased Chl b content and decreased Pn was also found. Remarkably, two mutants behaved significantly decreased Chl b content but increased Pn when compared that of Yugu 1. Seven mutants behaved significantly decreased Gs but non-decreasing Pn when compared that of Yugu 1.  The current study thus identified various genetic lines, further exploration of which would be beneficial to elucidate the relationship between Chl content, Gs and Pn and the mechanism underlying why C4 species are efficient at photosynthesis and water saving.

Keywords:  photosynthetic capacity        chlorophyll content        stomatal conductance        EMS mutant variation        Setaria italica  
Received: 03 June 2022   Online: 01 November 2022   Accepted: 18 August 2022
Fund: This work was supported by the National Natural Science Foundation of China (32241042 and 31771807), the National Key R&D Program of China (2021YFF1000103), the China Agricultural Research System (CARS-06-04), and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.
About author:  TANG Chan-juan, E-mail: 1406044224@qq.com; #Correspondence DIAO Xian-min, E-mail: diaoxianmin@caas.cn * These authors contribute equally to this study.

Cite this article: 

TANG Chan-juan, LUO Ming-zhao, ZHANG Shuo, JIA Guan-qing, TANG Sha, JIA Yan-chao, ZHI Hui, DIAO Xian-min. 2023. Variations in chlorophyll content, stomatal conductance and photosynthesis in Setaria EMS mutants. Journal of Integrative Agriculture, 22(6): 1618-1630.

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