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| Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene (ZmALDH7B6) in Response to Abiotic Stresses |
| AN Xia, DUAN Feng-ying, GUO Song, CHEN Fan-jun, YUAN Li-xing , GU Ri-liang |
| Department of Plant Nutrition, Center for Resources, Environment and Food Security, China Agricultural University, Beijing 100193, P.R.China |
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摘要 Aldehyde dehydrogenases (ALDHs) represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of the ALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maize ALDH7 subfamily member (ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-specific and stress-induced expression patterns, the 1.5-kb 5´-flanking ZmALDH7B6 promoter region was fused to the β-glucuronidase (GUS) reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply, confirming ammonium-dependent expression of ZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested that ZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.
Abstract Aldehyde dehydrogenases (ALDHs) represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of the ALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maize ALDH7 subfamily member (ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-specific and stress-induced expression patterns, the 1.5-kb 5´-flanking ZmALDH7B6 promoter region was fused to the β-glucuronidase (GUS) reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply, confirming ammonium-dependent expression of ZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested that ZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.
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Received: 15 March 2013
Accepted:
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| Fund: This work was financially supported by the National 863 Program of China (2012AA100306), the National 973 Program of China (2011CB100305), the National Natural Science Foundation of China (30971863, 31121062), and the Ministry of Agriculture of China (2011ZX08003-005). |
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Corresponding Authors:
GU Ri-liang, Tel: +86-10-62734424, Fax: +86-10-62731016, E-mail: rilianggu@cau.edu.cn
E-mail: rilianggu@cau.edu.cn
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| About author: AN Xia, E-mail: anxia0217@163.com |
Cite this article:
AN Xia, DUAN Feng-ying, GUO Song, CHEN Fan-jun, YUAN Li-xing , GU Ri-liang.
2014.
Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene (ZmALDH7B6) in Response to Abiotic Stresses. Journal of Integrative Agriculture, 13(9): 1900-1908.
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| Bartels D. 2001. Targeting detoxification pathways: An efficient approach to obtain plants with multiple stress tolerance? Trends in Plant Science, 6, 284-286 Bouche N, Fait A, Bouchez D, Moller S G, Fromm H. 2003. Mitochondrial succinic-semialdehyde dehydrogenase of the gamma-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants. Proceedings of the National Academy of Sciences of the United States of America, 100, 6843-6848 Brocker C, Vasiliou M, Carpenter S, Carpenter C, Zhang Y, Wang X, Kotchoni S O, Wood A J, Kirch H H, Kope?ný D, Nebert D W, Vasiliou V. 2012. Aldehyde dehydrogenase (ALDH) superfamily in plants: Gene nomenclature and comparative genomics. Planta, 237, 189-210 Chen X, Zeng Q, Wood A J. 2002. The stress-responsive Tortula ruralis gene ALDH21A1 describes a novel eukaryotic aldehyde dehydrogenase protein family. Journal of Plant Physiology, 159, 677-684 Czechowski T, Stitt M, Altmann T, Udvardi M K, Scheible W R. 2005. Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiology, 139, 5-17 Duncan D R, Williams M E, Zehr B E, Widholm J M. 1985. The production of callus capable of plant regeneration from immature embryos of numerous Zea mays (L.) genotypes. Planta, 165, 322-332 Gao C X, Han B. 2009. Evolutionary and expression study of the aldehyde dehydrogenase (ALDH) gene superfamily in rice (Oryza sativa). Gene, 431, 86-94 Gu R L, Zhao L, Zhang Y, Chen X P, Bao J, Zhao J F, Wang Z Y, Fu J J, Liu T S, Wang J H, Wang G Y. 2006. Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Plant Cell Reports, 25, 1157-1165 Huang W, Ma X, Wang Q, Gao Y, Xue Y, Niu X, Yu G, Liu Y. 2008. Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays). Plant Molecular Biology, 68, 451-463 Jimenez-Lopez J C, Gachomo E W, Seufferheld M J, Kotchoni S O. 2010. The maize ALDH protein superfamily: linking structural features to functional specificities. BMC Structure Biology, 10, 1-14 Kirch H H, Bartels D, Wei Y, Schnable P S, Wood A J. 2004. The ALDH gene superfamily of Arabidopsis. Trends Plant in Science, 9, 371-377 Kirch H H, Nair A, Bartels D. 2001. Novel ABA- and dehydration-inducible aldehyde dehydrogenase genes isolated from the resurrection plant Craterostigma plantagineum and Arabidopsis thaliana. Plant Journal, 28, 555-567 Kotchoni S O, Kuhns C, Ditzer A, Kirch H H, Bartels D. 2006. Over-expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress. Plant, Cell & Environment, 29, 1033- 1048. Laloi C, Apel K, Danon A. 2004. Reactive oxygen signalling: The latest news. Current Opinion in Plant Biology, 7, 323-328 Laszlo I, Szoke E, Tyihak E. 1998. Relationship between abiotic stress and formaldehyde concentration in tissue culture of Datura innoxia Mill. Journal of Plant Growth Regulation, 25, 195-199 Lindahl R. 1992. Aldehyde dehydrogenases and their role in carcinogenesis. Critical Reviews in Biochemistry and Molecular Biology, 22, 283-355 Nakazono M, Tsuji H, Li Y, Saisho D, Arimura S, Tsutsumi N, Hirai A. 2000. Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions. Plant Physiology, 124, 587-598 Reinehr R, Görg B, Becker S, Qvartskhava N, Bidmon H J, Selbach O, Haas H L, Schliess F, Häussinger D. 2007. Hypoosmotic swelling and ammonia increase oxidative stress by NADPH oxidase in cultured astrocytes and vital brain slices. GLIA, 55, 758-771 Rodrigues, S M, Andrade M O, Gomes A P, Damatta F M, Baracat-Pereira M C, Fontes E P. 2006. Arabidopsis and tobacco plants ectopically expressing the soybean antiquitin-like ALDH7 gene display enhanced tolerance to drought, salinity, and oxidative stress. Journal of Experimental Botany, 57, 1909-1918 Sambrook J, Russell D W. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press, New York, USA. Schauenstein E, Esterbauer H, Zollner H. 1977. Aldehydes in Biological Systems: Their Natural Occurrence and Biological Activities. Pion LTD Press, London, UK. Skibbe D S, Liu F, Wen T J, Yandeau M D, Cui X, Cao J, Simmons C R, Schnable P S. 2002. Characterization of the aldehyde dehydrogenase gene families of Zea mays and Arabidopsis. Plant Molecular Biology, 48, 751-764 Skopelitis D S, Paranychianakis N V, Paschalidis K A, Pliakonis E D, Delis I D, Yakoumakis D I, Kouvarakis A, Papadakis A K, Stephanou E G, Roubelakis-Angelakis K A. 2006. Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. The Plant Cell, 18, 2767-2781 Sophos N A, Vasiliou V 2003. Aldehyde dehydrogenase gene superfamily: the 2002 update. Chemico-Biological Interactions, 143, 5-22. Sunkar R, Bartels D, Kirch H H. 2003. Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. The Plant Journal, 35, 452-464 Wacker M, Wanek P, Eder E. 2001. Detection of 1,N2- propanodeoxyguanosine adducts of trans-4-hydroxy-2- nonenal after gavage of trans-4-hydroxy-2-nonenal or induction of lipid peroxidation with carbon tetrachloride in F344 rats Chemico Biological Interactions, 137, 269-283. Weber H, Chételat A, Reymond P, Farmer E. 2004. Selective and powerful stress gene expression in Arabidopsis in response to malondialdehyde. The Plant Journal, 37, 877-888 Wei M, Chao Y, Kao C. 2013. NaCl-induced heme oxygenase in roots of rice seedlings is mediated through hydrogen peroxide. Journal of Plant Growth Regulation, 69, 209- 214. Yoshida A, Rzhetsky A, Hsu L C, Chang C. 1998. Human aldehyde dehydrogenase gene family. European Journal of Biochemistry, 251, 549-557 Zhou M L, Zhang Q, Zhou M, Qi L P, Yang X B, Zhang K X, Pang J F, Zhu X M, Shao J R, Tang Y X, Wu Y M. 2012. Aldehyde dehydrogenase protein superfamily in maize. Functional & Integrative Genomics, 12, 683-692 |
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