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| Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China |
| WANG Fang, GAO Zheng-liang, AN Meng-nan, ZHOU Ben-guo , WU Yuan-hua |
1.College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China
2.Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China |
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摘要 Complete genome sequence of Potato virus Y Liaoning isolate (PVY-LN) causing tobacco vein necrosis symptoms were isolated from Liaoning Province in China. Genome sequences of PVY-LN was 9 714 nucleotides in length, excluding the 3´- terminal poly (A) tail. PVY-LN encodes a single long open reading frame (ORF) of polyprotein that is predicted to be cleaved into ten mature proteins by three viral proteases. No recombination can be predicted in PVY-LN sequences compared with that of the other PVY strains using Recombination Detection Programe v. 4.16 (RDP4). Complete genome sequence comparison and phylogenetic analysis indicated that PVY-LN is closely related to PVY necrosis strain (PVYN).
Abstract Complete genome sequence of Potato virus Y Liaoning isolate (PVY-LN) causing tobacco vein necrosis symptoms were isolated from Liaoning Province in China. Genome sequences of PVY-LN was 9 714 nucleotides in length, excluding the 3´- terminal poly (A) tail. PVY-LN encodes a single long open reading frame (ORF) of polyprotein that is predicted to be cleaved into ten mature proteins by three viral proteases. No recombination can be predicted in PVY-LN sequences compared with that of the other PVY strains using Recombination Detection Programe v. 4.16 (RDP4). Complete genome sequence comparison and phylogenetic analysis indicated that PVY-LN is closely related to PVY necrosis strain (PVYN).
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Received: 12 November 2012
Accepted:
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| Fund: This work was supported by the Programs for Science and Technology of State Tobacco Monopoly Administration, China ([2010]182) and the Science and Technology of Liaoning Province Tobacco Monopoly Administration, China ([2010]86) and the Key Programe of Anhui Province Tobacco Monopoly Administration, China (20100551005). |
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Corresponding Authors:
Correspondence WU Yuan-hua, Tel: +86-24-88487359, Fax: +86-24-88487359, E-mail: wuyh09@vip.sina.com
E-mail: wuyh09@vip.sina.com
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| About author: WANG Fang, Tel: +86-551-65148963, Fax: +86-551-65148963, E-mail: wangfang1378@126.com; |
Cite this article:
WANG Fang, GAO Zheng-liang, AN Meng-nan, ZHOU Ben-guo , WU Yuan-hua.
2013.
Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China. Journal of Integrative Agriculture, 12(7): 1195-1200.
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