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Journal of Integrative Agriculture  2023, Vol. 22 Issue (1): 31-40    DOI: 10.1016/j.jia.2022.08.101
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Less hairy leaf 1, an RNaseH-like protein, regulates trichome formation in rice through auxin
CHEN Hong-yan*, ZHU Zhu*, WANG Xiao-wen, LI Yang-yang, HU Dan-ling, ZHANG Xue-fei, JIA Lu-qi, CUI Zhi-bo, SANG Xian-chun
Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.China
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摘要  水稻叶片表皮毛是由表皮细胞分化发育形成,表皮毛在植物的抗逆以及防止紫外直射等过程中都具有重要的作用,但关于水稻毛状体发育的研究还存在很大未知。在本研究中,我们对野生型籼系水稻西大1B进行EMS (ethylmethane sulfonate) 诱导,通过表型观察分析筛选出了毛状体发育缺陷突变体,将其命名为lhl1 (Less Hairy Leaf 1)。我们对其进行了基因定位和图位克隆,将其定位在2号染色体两个分子标记的70 kb的区间内,通过基因测序将LOC_Os02g25230确定为候选基因。之后我们构建了干涉以及超表达株系,扫描电镜观察分析发现LHL1-RNAi叶片同lhl1一样存在毛状体发育缺陷,但LHL1-OE株系叶片表面的毛状体形态与野生型相似,但数目大大增加。qRT-PCR分析发现,与野生型相比,突变体lhl1中正向调控毛状体发育相关的基因表达下调。对生长素相关基因定量分析发现,突变体hl7中生长素相关基因的表达严重下调,进一步通过激素响应分析发现HL7的表达受到生长素的诱导,证明了HL7影响水稻叶片毛状体的发育可能与生长素途径有关。

Abstract  The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.  Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.  However, the development of rice trichomes remains poorly understood.  In this study, we conducted ethylmethane sulfonate (EMS)-mediated mutagenesis on the wild-type (WT) indica rice ‘Xida 1B’.  Phenotypic analysis led to the screening of a mutant that is defective in trichome development, designated lhl1 (less hairy leaf 1).  We performed map-based cloning and localized the mutated gene to the 70-kb interval between the molecular markers V-9 and V-10 on chromosome 2.  The locus LOC_Os02g25230 was identified as the candidate gene by sequencing.  We constructed RNA interference (LHL1-RNAi) and overexpression lines (LHL1-OE) to verity the candidate gene.  The leaves of the LHL1-RNAi lines showed the same trichome developmental defects as the lhl1 mutant, whereas the trichome morphology on the leaf surface of the LHL1-OE lines was similar to that of the WT, although the number of trichomes was significantly higher.  Quantitative real-time PCR (RT-qPCR) analysis revealed that the expression levels of auxin-related genes and positive regulators of trichome development in the lhl1 mutant were down-regulated compared with the WT.  Hormone response analysis revealed that LHL1 expression was affected by auxin.  The results indicate that the influence of LHL1 on trichome development in rice leaves may be associated with an auxin pathway.
Keywords:  rice        trichome        gene cloning        auxin  
Received: 29 July 2020   Accepted: 28 September 2021
Fund: This work was supported by grants from the Natural Science Foundation of Chongqing, China (cstc2020jcyj-msxm0539, cstc2015jcyjA80008), the National College Students Innovation and Entrepreneurship Training Program from the Ministry of Education, China (202110635082), and the National Natural Science Foundation of China (32171964, 31171178).
About author:  CHEN Hong-yan, E-mail:;

Cite this article: 

CHEN Hong-yan, ZHU Zhu, WANG Xiao-wen, LI Yang-yang, HU Dan-ling, ZHANG Xue-fei, JIA Lu-qi, CUI Zhi-bo, SANG Xian-chun. 2023. Less hairy leaf 1, an RNaseH-like protein, regulates trichome formation in rice through auxin. Journal of Integrative Agriculture, 22(1): 31-40.

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