|
|
|
|
|
| Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella |
| ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui |
1、Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, P.R.China
2、College of Animal Science and Technology, Southwest University, Chongqing 400715, P.R.China |
|
|
|
摘要 Toll-like receptors (TLRs) are a group of highly conserved molecules which initiate the innate immune response to pathogens by recognizing structural motifs of microbes. Understanding the changes in chicken Toll-like receptors (ChTLRs) and signal adaptors expression that occur with Eimeria tenella infection will help to elucidate the molecular basis of immune control of coccidiosis caused by Eimeria. The present study detected the dynamic changes in the expression of ChTLRs and associated signal adaptors in the spleen and cecum of E. tenella-infected chickens during the early stage of infection. The results showed that the expression peak for ChTLRs, MyD88 and TRIF occurred at 12 h post-infection (hpi), ChTLR3, ChTLR15 and MyD88 mRNA expression in the spleen of E. tenella infected chickens were significantly higher (P<0.05) than that of negative control chickens, and there were similar tendencies of these molecules expression in the cecum and spleen of E. tenella-infected chickens. The expression of MyD88 was upregulated at four time points in the cecum of E. tenella-infected chickens. The results of this study indicate that ChTLR3, ChTLR15 and MyD88 play a role in young chickens infected with E. tenella.
Abstract Toll-like receptors (TLRs) are a group of highly conserved molecules which initiate the innate immune response to pathogens by recognizing structural motifs of microbes. Understanding the changes in chicken Toll-like receptors (ChTLRs) and signal adaptors expression that occur with Eimeria tenella infection will help to elucidate the molecular basis of immune control of coccidiosis caused by Eimeria. The present study detected the dynamic changes in the expression of ChTLRs and associated signal adaptors in the spleen and cecum of E. tenella-infected chickens during the early stage of infection. The results showed that the expression peak for ChTLRs, MyD88 and TRIF occurred at 12 h post-infection (hpi), ChTLR3, ChTLR15 and MyD88 mRNA expression in the spleen of E. tenella infected chickens were significantly higher (P<0.05) than that of negative control chickens, and there were similar tendencies of these molecules expression in the cecum and spleen of E. tenella-infected chickens. The expression of MyD88 was upregulated at four time points in the cecum of E. tenella-infected chickens. The results of this study indicate that ChTLR3, ChTLR15 and MyD88 play a role in young chickens infected with E. tenella.
|
|
Received: 11 October 2012
Accepted:
|
| Fund: This work was supported by the Fundamental Research Funds for the Central Universities, China (XDJK2010C099), the Science Fundation for Young Scientists of Southwest University, China (QNRC200804) and the Scientific Research Fund of Veterinary Medicine Department of Southwest University, China. |
|
Corresponding Authors:
NIE Kui, Tel/Fax: +86-23-68251196, E-mail: niekui@126.com
|
| About author: ZHOU Zuo-yong, Tel: +86-23-46751100, E-mail: zzyxnny@163.com |
Cite this article:
ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui.
2014.
Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella. Journal of Integrative Agriculture, 13(4): 904-910.
|
| Abasht B, Kaiser M G, Lamont S J. 2008. Toll-like receptor gene expression in cecum and spleen of advanced intercross line chicks infected with Salmonella enterica serovar Enteritidis. Veterinary Immunology and Immunopathology, 123, 314-323 Albiger B, Dahlberg S, Henriques-Normark B, Normark S. 2007. Role of the innate immune system in host defence bacterial infections: focus on the Toll-like receptors. Journal of Internal Medicine, 261, 511-528 Augustine P C, Danforth H D. 1986. A study of the dynamics of the invasion of immunized birds by Eimeria sporozoites. Avian Disease, 30, 347-351 Brisbin J T, Zhou H, Gong J, Sabour P, Akbari M R, Haghighi H R, Yu H, Clarke A, Sarson A J, Sharif S. 2008. Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components. Developmental and Comparative Immunology, 32, 563-574 Brownlie R, Allan B. 2011. Avian toll-like receptors. Cell and Tissue Research, 343, 121-130 Ciraci C, Lamont S J. 2011. Avian-specific TLRs and downstream effector responses to CpG-induction in chicken macrophages. Developmental and Comparative Immunology, 35, 392-398 Dalloul R A, Lillehoj H S. 2005. Recent advances in immunomodulation and vaccination strategies against coccidiosis. Avian Disease, 49, 1-8 Del C E, Gallego M, Lee S H, Lillehoj H S, Quilez J, Lillehoj E P, Sanchez-Acedo C. 2011. Induction of protective immunity against Eimeria tenella infection using antigen-loaded dendritic cells (DC) and DC- derived exosomes. Vaccine, 29, 3818-3825 Gazzinelli RT, Denkers E Y. 2006. Protozoan encounters with Toll-like receptor signalling pathways: implications for host parasitism. Nature Reviews Immunology, 6, 895- 906. Higgs R, Cormican P, Cahalane S, Allan B, Lloyd A T, Meade K, James T, Lynn D J, Babiuk L A, O’farrelly C. 2006. Induction of a novel chicken Toll-like receptor following Salmonella enterica serovar typhimurium infection. Infection and Immunity, 74, 1692-1698 Higuchi M, Matsuo A, Shingai M, Shida K, Ishii A, Funami K, Suzuki Y, Oshiumi H, Matsumoto M, Seya T. 2008. Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily. Developmental and Comparative Immunology, 32, 147- 155. Hong Y H, Lillehoj H S, Lillehoj E P, Lee S H. 2006. Changes in immune-related gene expression and intestinal lymphocyte subpopulations following Eimeria maxima infection of chickens. Veterinary Immunology and Immunopathology, 114, 259-272 Jeurissen S H, Janse E M, Vermeulen A N, Vervelde L. 1996. Eimeria tenella infections in chickens: aspects of host-parasite: interaction. Veterinary Immunology and Immunopathology, 54, 231-238 Kawai T, Akira S. 2007. TLR signaling. Seminars in Immunology, 19, 24-32 Keestra A M, de Zoete M R, van Aubel R A, van Putten J P. 2008. Functional characterization of chicken TLR5 reveals species-specific recognition of flagellin. Molecular Immunology, 45, 1298-1307 Kropf P, Freudenberg M A, Modolell M, Price H P, Herath S, Antoniazi S, Galanos C, Smith D F, Muller I. 2004. Toll-like receptor 4 contributes to efficient control of infection with the protozoan parasite Leishmania major. Infection and Immunity, 72, 1920-1928 Kumar H, Kawai T, Akira S. 2009. Toll-like receptors and innate immunity. Biochemical and Biophysical Research Communications, 388, 621-625 Li P, Xia P, Wen J, Zheng M, Chen J, Zhao J, Jiang R, Liu R, Zhao G. 2010. Up-regulation of the MyD88-dependent pathway of TLR signaling in spleen and caecum of young chickens infected with Salmonella serovar Pullorum. Veterinary Microbiology, 143, 346-351 MacKinnon K M, He H, Nerren J R, Swaggerty C L, Genovese K J, Kogut M H. 2009. Expression profile of toll-like receptors within the gastrointestinal tract of 2-day-old Salmonella enteriditis-infected broiler chickens. Veterinary Microbiology, 137, 313-319 Medzhitov R. 2001. Toll-like receptors and innate immunity. Nature Reviews Immunology, 1, 135-145 O’Neill L A, Fitzgerald K A, Bowie A G. 2003. The Toll- IL-1 receptor adaptor family grows to five members Trends in Immunology, 24, 286-290 Shaughnessy R G, Meade K G, Cahalane S, Allan B, Reiman C, Callanan J J, O’Farrelly C. 2009. Innate immune gene expression differentiates the early avian intestinal response between Salmonella and Campylobacter. Veterinary Immunology and Immunopathology, 132, 191-198 Sumners L H, Miska K B, Jenkins M C, Fetterer R H, Cox C M, Kim S, Dalloul R A. 2011. Expression of Toll-like receptors and antimicrobial peptides during Eimeria praecox infection in chickens. Experimental Parasitology, 127, 714-718 Swaggerty C L, Genovese K J, He H, Duke S E, Pevzner I Y, Kogut M H. 2011. Broiler breeders with an efficient innate immune response are more resistant to Eimeria tenella. Poultry Science, 90, 1014-1019 Temperley N D, Berlin S, Paton I R, Griffin D K, Burt D W. 2008. Evolution of the chicken Toll-like receptor gene family: a story of gene gain and gene loss. BMC Genomics, 9, 62. Totemeyer S, Foster N, Kaiser P, Maskell D J, Bryant C E. 2003. Toll-like receptor expression in C3H/HeN and C3H/HeJ mice during Salmonella enterica serovar typhimurium infection. Infection and Immunity, 71, 6653-6657 Williams R B. 2002. Anticoccidial vaccines for broiler chickens: pathways to success. Avian Pathology, 31, 317-353 Zhang L, Liu R, Ma L, Wang Y, Pan B, Cai J, Wang M. 2012. Eimeria tenella: expression profiling of toll- like receptors and associated cytokines in the cecum of infected day-old and three-week old SPF chickens. Experimental Parasitology, 130, 442-448 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
