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| Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize |
| YU Jiang-hua, ZHAO Yan-xin, QIN Ya-ting, YUE Bing, ZHENG Yong-lian , XIAO Hai-lin |
| National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R.China |
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摘要 MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value<0.01 and 0.011.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.
Abstract MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value<0.01 and 0.011.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.
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Received: 19 April 2012
Accepted:
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| Fund: This study was financially supported by the National Natural Science Foundation of China (31171565). |
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Corresponding Authors:
Correspondence XIAO Hai-lin, Mobile: 18627050215, E-mail: hailinxiao@gmail.com
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| About author: YU Jiang-hua, Tel: +86-27-87282689, E-mail: jianghuayu@gmail.com |
Cite this article:
YU Jiang-hua, ZHAO Yan-xin, QIN Ya-ting, YUE Bing, ZHENG Yong-lian , XIAO Hai-lin.
2013.
Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize. Journal of Integrative Agriculture, 12(2): 229-238.
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