Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization

doi:10.1016/S1671-2927(00)9057

Journal of Integrative Agriculture

2013, Vol. 12

Issue (8): 1431-1440 DOI: 10.1016/S1671-2927(00)9057

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Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization

GAO Feng, NIU Yi-ding, HAO Jin-feng, BADE Rengui, ZHANG Li-quan , HASI Agula

Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology/College of Life Sciences, Inner Mongolia University, Hohhot 010021, P.R.China

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摘要 Melon (Cucumis melo L.) is an important horticultural crop worldwide. Ethylene regulates the ripening process and affects the ripening rate. To screen genes that are differentially expressed at the burst of ethylene climacteric in melon fruit, we performed suppression subtractive hybridization (SSH) to generate forward and reverse libraries, for which we sequenced 439 and 445 clones, respectively. Our BLAST analysis showed that the genes from the 2 libraries were involved in metabolism, signal transduction, cell structure, transcription, translation, and defense. Six genes were analyzed by qRT-PCR during the differential developmental stage of melon fruit. Our results provide new insight into the understanding of climacteric ripening of melon fruit.

Abstract Melon (Cucumis melo L.) is an important horticultural crop worldwide. Ethylene regulates the ripening process and affects the ripening rate. To screen genes that are differentially expressed at the burst of ethylene climacteric in melon fruit, we performed suppression subtractive hybridization (SSH) to generate forward and reverse libraries, for which we sequenced 439 and 445 clones, respectively. Our BLAST analysis showed that the genes from the 2 libraries were involved in metabolism, signal transduction, cell structure, transcription, translation, and defense. Six genes were analyzed by qRT-PCR during the differential developmental stage of melon fruit. Our results provide new insight into the understanding of climacteric ripening of melon fruit.

Keywords: Cucumis melo ethylene climacteric fruit ripening differentially expressed mRNA gene expression suppression subtractive hybridization

Received: 18 September 2012 Accepted:

Fund:

This work was supported by the National Natural Science Foundation of China (30960159) and the Specialized Research Foundation for the Doctoral Program of Higher Education (200801260002).

Corresponding Authors: Correspondence HASI Agula, Tel: +86-471-4992209, Fax: +86-471-4992435, E-mail: hasind@sina.com E-mail: hasind@sina.com

About author: GAO Feng, E-mail: imgaofeng@163.com

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GAO Feng, NIU Yi-ding, HAO Jin-feng, BADE Rengui, ZHANG Li-quan , HASI Agula. 2013. Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization. Journal of Integrative Agriculture, 12(8): 1431-1440.

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