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| Identification of QTLsAssociated with Total SoyasaponinContent in Soybean (Glycine max (L.) Merr.) |
| HUANG Shan-shan, HAN Ying-peng, LI Chang-suo, TIAN Jun, LI Wen-bin, WANG Ji-an |
1.Key Laboratory of Soybean Biology, Ministry of Education/Soybean Research Institute, Northeast Agricultural University, Harbin 150030, P.R.China
2.Plant Protection Institute, Heilongjiang Academy of Land Reclamation Sciences, Harbin 150038, P.R.China |
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摘要 Soyasaponins are valuable compounds in certain drugs, industry, food additives and surfactants. Selecting cultivars with higher-soyasaponin content along with agronomic traits is a main goal for many soybean breeders. The aim of the present study was to identify the quantitative trait loci (QTLs) associated with total soyasaponin content through a F2 population, which was derived from a cross between Ha 91016 (higher soyasaponin content cultivar, 16.8 mg g-1) and N98-9445A (lower soyasaponin content, only 5.7 mg g-1). A genetic linkage map including a total of 162 simple sequence repeat markers was constructed, which covered the total length 2 735.5 cM, and the average distance between markers was 16.96 cM. Two QTLs associated with total soyasaponin content were identified. One, qSAP_1 (located in sat_044-satt102 of linkage group (LG) K), could explain 12.6% of phenotypic variance. The other, qSAP_2, was located between satt368 and sat_413 of LG D1a, which could explain 15.8% of phenotypic variance. It was concluded that the two QTLs would have some potential value for marker-assisted selection for high-soyasaponin content breeding in soybeans.
Abstract Soyasaponins are valuable compounds in certain drugs, industry, food additives and surfactants. Selecting cultivars with higher-soyasaponin content along with agronomic traits is a main goal for many soybean breeders. The aim of the present study was to identify the quantitative trait loci (QTLs) associated with total soyasaponin content through a F2 population, which was derived from a cross between Ha 91016 (higher soyasaponin content cultivar, 16.8 mg g-1) and N98-9445A (lower soyasaponin content, only 5.7 mg g-1). A genetic linkage map including a total of 162 simple sequence repeat markers was constructed, which covered the total length 2 735.5 cM, and the average distance between markers was 16.96 cM. Two QTLs associated with total soyasaponin content were identified. One, qSAP_1 (located in sat_044-satt102 of linkage group (LG) K), could explain 12.6% of phenotypic variance. The other, qSAP_2, was located between satt368 and sat_413 of LG D1a, which could explain 15.8% of phenotypic variance. It was concluded that the two QTLs would have some potential value for marker-assisted selection for high-soyasaponin content breeding in soybeans.
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Received: 06 September 2011
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (30471092). |
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Corresponding Authors:
Correspondence WANG Ji-an, Tel: +86-451-55190692, E-mail: wangsoy@yahoo.com.cn
E-mail: wangsoy@yahoo.com.cn
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| About author: HUANG Shan-shan, E-mail: huangshanshan.6@163.com; |
Cite this article:
HUANG Shan-shan, HAN Ying-peng, LI Chang-suo, TIAN Jun, LI Wen-bin, WANG Ji-an.
2012.
Identification of QTLsAssociated with Total SoyasaponinContent in Soybean (Glycine max (L.) Merr.). Journal of Integrative Agriculture, 12(12): 1976-1984.
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| [1]Agrell J, Oleszek W, Stochmal A, Olsen M, Anderson P.2003. Herbivore-induced responses in alfalfa(Medicago sativa). Journal of Chemical Ecology, 29,303-320.[2]Ahn K S, Kim J H, Oh S R, Min B S, Kinjo J, Lee H K. 2002.Effects of oleanane-type triterpenoids from Fabaceousplants on the expression of ICAM-1. Biological andPharmaceutical Bulletin, 25, 1105-1107.[3]Allen F L. 1994. Usefulness of plant genome mapping toplant breeding. In: Gressho V P, ed., Plant GenomeAnalysis. CRC Press, Boca Raton. pp. 11-18.[4]Anna S, Cezary P, Czkowski, Max H. 2010. Influence ofenvironmental abiotic factors on the content ofsaponins in plants. Phytochemistry Reviews, 10, 471-491.[5]Balandrin MF. 1996. Commercial utilization of plant-derivedsaponins: an overview of medicinal, pharmaceutical andindustrial applications. Advances in ExperimentalMedicine and Biology, 404, 1-14.[6]Berhow M A, Wagner E D, Vaughn S F, Plewa M J. 2000.Characterization and antimutagenic activity of soybeansaponin. Mutation Research, 448, 11-22.[7]Cheeke P R. 2000. Actual and potential applications of Yuccaschidigera and Quillaja saponaria saponins in humanand animal nutrition. Journal of Animal Science, 77, 1-10.[8]Cregan P B, Jarvik T, Bush A L, Shoemaker R C, Lark K G,Kahler A L, VanToai T T, Lohnes D G, Chung J, SpechtJ E. 1999. An integrated genetic linkage map of thesoybean genome. Crop Science, 39, 1464-1490.[9]Decroos K, Vincken J P. 2005. Simultaneous quantification ofdifferently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-oneconjugatedsoyasaponins using reversed-phase high-performanceliquidchromatography with evaporative light scatteringdetection. Journal of Chromatography (A), 1072, 185-193.[10]Doyle J J, Doyle J L. 1990. Isolation of plant DNA fromfresh tissue. Focus, 12, 13-15.[11]Dubois M A, Wagner H. 1996. A review of the biologicaland pharmacological ac t ivi t i es of saponins .Phytomedicine, 2, 363-386.[12]Fenwick D E, Oakenfull D. 1983. Saponin content of foodplants and some prepared foods. Journal of the Scienceof Food and Agriculture, 34, 186-191.[13]Fenwick D E, Oakenfull D. 2006. Saponin content of foodplants and some prepared foods. Journal of the Scienceof Food and Agriculture, 34, 186-191.[14]Francis G, Kerem Z, Makkar H P S, Becker K. 2002. Thebiological action of saponins in animal systems: a review.The British Journal of Nutrition, 88, 587-605.[15]Gauthier C, Legault J, Pichette A. 2009. Recent progress inthe synthesis of naturally occurring triterpenoidsaponins. Mini-Reviews in Organic Chemistry, 6, 321-344.[16]Golawska S, Leszczynski B, OleszekW. 2006. Effect of lowand high-saponin lines of alfalfa on pea aphid. Journalof Insect Physiology, 52, 737-743.[17]Gurfinkel D M, Rao A V. 2003. Soyasaponins: therelationship between chemical structure and colonanticarcinogenic activity. Nutrition and Cancer, 47,24-33.[18]Hanausek M, Ganesh P, Walaszek Z, Arntzen C J, Slaga T J,Gutterman J U. 2001. Avicins, a family of triterpenoidsaponins from Acacia victoriae (Bentham), suppressHit ras mutations and aneuploidy in a murine skincarcinogenesis model. Proceedings of the NationalAcademy of Sciences of the United States of America,98, 11551-11556.[19]Haralampidis K, Trojanowska M, Osbourn A E. 2002.Biosynthesis of triterpenoid saponins in plants.Advances in Biochemical Engineering/Biotechnology,75, 31-48.[20]Henry M. 2005. Saponins and phylogeny: example of the“gypsogenin group” saponins. Phytochemistry Reviews,4, 89-94.[21]Hostettmann K, Marston A. 1995. Saponins. CambridgeUniverisity Press, England.Hussein A H, Geung J L, Roger H, Boerma. 2011.Identification of QTL for increased fibrous roots insoybean. Theoretical and Applied Genetics, 122, 935-946.[22]Irma P, Agnieszka G, Danuta S. 2010. Saponins as cytotoxicagents: a review. Phytochemistry Reviews, 9, 425-474.[23]Kalinowska M, Zimowski J, Paficzkowski C. 2005. Theformation of sugar chains in triterpenoid saponins andglycoalkaloids. Phytochemistry Reviews, 4, 237-257.[24]Kinjo J, Yokomizo K, Hirakawa T, Shii Y, Nohara T, Uyeda M.2000. Anti-herpes virus activity of Fabaceoustriterpenoidal saponins. Biological and PharmaceuticalBulletin, 23, 887-889.[25]Kitagawa I. 1985. Histochemistry soyasaponins insoybeans. Chemical and Pharmaceutical Bulletin, 33,3829-3833.[26]Kudou S, Tonomura M, Tsukamoto C, Uchida T, Sakabe T,Tamura N, Okubo K. 1993. Bioscience. Biotechnologyand Biochemistry, 57, 546-550.[27]Lander E S, Green P, Abrahamson J, Barlow A, Daly M,Lincoln S, Newburg L. 1987. Mapmaker: an interactivecomputer package for constructing primary geneticlinkage maps of experimental and natural populations.Genomics, 1, 174-181.[28]Miura N, Matsumoto Y, Miyairi S, Nishiyama S, NaganumaA. 1999. Protective effects of triterpene compoundsagainst the cytotoxicity of cadmium in HepG2 cells.Molecular Pharmacology, 56, 1324-1328.[29]Oleszek W, Bialy Z. 2006. Chromatographic determinationof plant saponins - an update (2002-2005). Journal ofChromatography (A), 1112, 78-91.[30]Osbourn A. 1996. Saponins and plant defence - a soapstory. Trends in Plant Science, 1, 4-9.[31]Patrick F, Dowd M A, Berhow E T, Johnson. 2011.Differential Activity of multiple saponins againstomnivorous insects with varying feeding preferences.Journal of Chemical Ecology, 37, 443-449.[32]Philip A. 1986. Saponins content of soya and somecommercial soya products by means of HPLC of thesapogenins. Journal of the Science of Food andAgriculture, 37, 694-69.[33]Rowlands J C, Berhow MA, Badger T M. 2002. Estrogenicand antiproliferative properties of soy sapogenols inhuman breast cancer cells in vitro. Food and ChemicalToxicology, 40, 1767-1774.[34]Sahu N P, Banerjee S, Mondal N B, Mandal D. 2008.Steroidal saponins. In: Fortschritte der ChemieOrganischer Naturstoffe/Progress in the Chemistry ofOrganic Natural Products. Springer,Vienna. pp. 45-141.[35]Shiraiwa M. 1991. Composition and structure of “group Asaponin” in soybean seed. Agricultural and BiologicalChemistry, 55, 315-322.[36]Shiraiwa M, Harada K, Okubo K. 1991. Composition andstructure of “Group B Saponin” in soybean seed.Agricultural and Biological Chemistry, 55, 911-917.[37]Skene C D, Sutton P. 2006. Saponin-adjuvanted particulatevaccines for clinical use. Methods, 40, 53-59.[38]Sparg S G, Light M E, Van S J. 2004. Biological activitiesand distribution of plant saponins. Journal ofEthnopharmacology, 94, 219-243.[39]Stuber C W, Lincoln S E, Wolff D W, Helentjaris T, LanderE S. 1992. Identification of genetic factors contributingto heterosis in a hybrid from two elite maize inbred linesusing molecular markers. Genetics, 132, 832-839.[40]Sun H X, Xie Y, Ye Y P. 2009. Advances in saponin-basedadjuvants. Vaccine, 27, 1787-1796.[41]Tanaka O, Tamura Y, Masuda H, Mizutani K. 1996.Application of saponins in food and cosmetics:saponins of Mohave yucca and Sapinus mukurossi.Advances in Experimental Medicine and Biology, 405,1-11.[42]Trigizano R N, Caetano A G. 1998. Laboratory exercises onDNA amplification fingerprinting for evaluating themolecular diversity of hort icultural species.Horttechnology, 8, 413-423.[43]Vincken J P, Heng L, Groot A. 2007. Saponins, classificationand occurence in the plant kingdom. Phyto-Chemistry,68, 275-297.[44]Wang Y, Zhang Y, Zhu Z. 2007. Exploration of thecorrelation between the structure, hemolytic activity,and cytotoxicity of steroid saponins. Bioorganic andMedicinal Chemistry, 15, 2528-2532.[45]Yang C, Zhao T J, Yu D Y, Gai J Y. 2011. Mapping QTLs fortissue culture response in soybean (Glycine max (L.)Merr.). Molecules and Cells, 32, 337-342.[46]Yang C R, Zhang Y, Jacob M R. 2006. Antifungal activity ofC-27 steroidal saponins. Antimicrobial Agents andChemotherapy, 50, 1710-1714.[47]Yang Y W, Jin M C, Huang M, Su B G, Ren Q L. 2007.Ultrasound-assisted extraction of soyasaponins fromhypocotyls, and analysis by LC-ESI-MS. Chromatographia,65, 555-560.[48]Yu B, Sun J. 2009. Current synthesis of triterpene saponins.Chemistry-An Asian Journal, 4, 642-654.[49]Yu B, Zhang Y, Tang P. 2007. Carbohydrate chemistry inthe total synthesis of saponins. European Journal ofOrganic Chemistry, 31, 5145-5161.[50]ZhangWK, Wang Y J, Zhang J S. 2004. QTLmapping of tenagronomic traits on the soybean (Glycine max (L.) merr).Genetic map and their association with EST markers.Theoretical and Applied Genetics, 108, 1131-113.[51]Zhao G Y, Wang J A, Han Y P, Teng W L, Sun G L, Li W B.2008. Identification of QTL underlying the resistanceof soybean to pod borer, Leguminivora glycinivorella(Mats.) obraztsov, and correlations with plant, pod andseed traits. Euphytica, 164, 275-282. |
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