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Journal of Integrative Agriculture  2012, Vol. 12 Issue (10): 1665-1674    DOI: 10.1016/S1671-2927(00)8699
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Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid
 XU You-ping, CHEN Hui-ying, ZHOU Xin, CAI Xin-zhong
1.Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2.Center of Analysis and Measurement, Zhejiang University, Hangzhou 310058, P.R.China
3.Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture/Zhejiang University, Hangzhou 310058,P.R.China
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摘要  Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an inducer of plant hypersensitive response (HR) and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNNI1 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNNI1 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfHNNI1 induced plant HR in a dose-dependent manner. CfHNNI1-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and def1 tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.

Abstract  Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an inducer of plant hypersensitive response (HR) and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNNI1 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNNI1 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfHNNI1 induced plant HR in a dose-dependent manner. CfHNNI1-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and def1 tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.
Keywords:  CfHNNI1       Cladosporium fulvum       ethylene       hypersensitive response       jasmonic acid       nonhost resistance       salicylic acid  
Received: 11 November 2011   Accepted:
Fund: 

This work was financially supported by grants from the National Basic Research Program of China (2009CB119000), the Genetically Modified Organisms Breeding Major Projects (2009ZX08009-044B), the PCSIRT Project (IRT0943), the Fundamental Research Funds for the Central Universities (2011XZZX006), and the Program for New Century 151 Talents of Zhejiang Province, China.

Corresponding Authors:  Correspondence CAI Xin-zhong, Tel: +86-571-88982936, Fax: +86-571-88982936, E-mail: xzhcai@zju.edu.cn     E-mail:  xzhcai@zju.edu.cn
About author:  XU You-ping, E-mail: ypxu@zju.edu.cn

Cite this article: 

XU You-ping, CHEN Hui-ying, ZHOU Xin, CAI Xin-zhong. 2012. Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid. Journal of Integrative Agriculture, 12(10): 1665-1674.

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