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| AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China |
| LIU De-gao, ZHAO Ning, ZHAI Hong, YU Xiao-xia, JIE Qin, WANG Lian-jun, HE Shao-zhen, LIU Qing-chang |
| Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China |
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摘要 AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between “Northern” and “Southern” sweetpotato varieties when Yangtze River was used as the dividing line.
Abstract AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between “Northern” and “Southern” sweetpotato varieties when Yangtze River was used as the dividing line.
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Received: 04 March 2011
Accepted:
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| Fund: This work was supported by China Agriculture Research System (Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008). |
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Corresponding Authors:
Correspondence LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
E-mail: liuqc@cau.edu.cn
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Cite this article:
LIU De-gao, ZHAO Ning, ZHAI Hong, YU Xiao-xia, JIE Qin, WANG Lian-jun, HE Shao-zhen, LIU Qing-chang.
2012.
AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China. Journal of Integrative Agriculture, 12(9): 1424-1433.
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