Abstract:

【Objective】 The objective of this study is to isolate genes related to fuzz initiation and development in Gossypium arboretum. 【Method】 The filter arrays of cDNAs were performed to identify the differentiation expressed genes between the wild-type DPL971 and its fuzz mutant line DPL972 in the early developing stages of -3DPA, 0DPA, +3DPA, +5DPA, and +7DPA. Compared with the database of model plant Arabodopsis thaliana, some genes related to the initiation of the cotton fiber were isolated, and part of them have been verified by RT-PCR and QRT-PCR. 【Result】 Eight genes related to fiber initiation and development in Gossypium arboretum were identified, and seven of them, KAK(DR461366), MYB5(ES812048), TTG1(ES811600), MYB23(DR453866), CSLD3(DR459646), RHD2(DR461821), ZWI(ES791383), were highly homologous to the Arabodopsis proteins which played important roles in the initiation of the trichomes development in Arabidopsis. The result of RT-PCR and QRT-PCR indicated that the 5 genes of MYB23, MYB5, TTG1, CSLD3, and RHD2 had significantly different expressions in +3DPA ovules between the fuzz mutant line DPL972 and its wild line DPL971. 【Conclusion】 It implied that these genes may be related to the initiation of the fuzz fiber, the first gene may inhibit the fuzz differentiation and the latter four may promote the fuzz development.

Key words: Gossypium arboretum, cDNA microarray, cotton fiber, fuzzless