Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (17): 3822-3841.doi: 10.3864/j.issn.0578-1752.2026.17.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Combination of Organic Fertilizer and Rotational Tillage Patterns on Nitrogen Migration and Transformation in Fluvo-Aquic Soil in Northern Henan Province

LI Shuo(), LIU ChaoLin(), LIU ShiLiang, JIANG GuiYing(), YANG Jin, WANG BeiBei, ZHAO Ying, ZHU XuanLin, LIU Fang, JIE XiaoLei   

  1. College of Resources and Environment, Henan Agricultural University/Key Laboratory of Arable Land Quality Conservation in the Huanghuaihai Plain, Ministry of Agriculture and Rural Affairs/State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Zhengzhou 450046
  • Received:2025-10-29 Accepted:2025-12-22 Online:2026-09-03 Published:2026-09-03
  • Contact: JIANG GuiYing

Abstract:

【Objective】Based on the long term tillage experiment, this study investigated the effects of combination of rotational tillage mode and fertilization on nitrogen migration and transformation, crop growth in fluvo-aquic soil in the northern Henan province, aiming to select the optimal agricultural management in this area.【Method】The long term tillage experiment with two-factor split-plot design was carried out. Fertilization with manure or not was the main factor and tillage mode as the subfactor. Four rotational tillage modes were set as: (1) Continuous rotary tillage (RT-RT-RT); (2) Deep tillage-rotary tillage-rotary tillage (DT-RT-RT); (3) Deep tillage-rotary tillage-shallow rotary tillage (DT-RT-SRT); (4) Deep tillage- shallow rotary tillage-shallow rotary tillage (DT-SRT-SRT). The soil nitrogen forms, enzyme activity, crop nitrogen utilization, and yield were determined and analyzed.【Result】Compared with RT-RT-RT with mineral fertilizer (NPK), all the nitrogen forms content were increased under DT-RT-SRT with manure (NPKM) in the 0-40 cm soil layer. Therein, total nitrogen (TN), nitrate nitrogen (NO3--N), and ammonium nitrogen (NH4+-N) content increased by 25.0%-49.1%, 42.8%-140.0%, and 4.6%-125.6%, respectively. In the 0-40 cm soil layer, the bulk density (BD) under NPKM was lower (0.3%-15.7%) than that under NPK, soil porosity (SR) increased by 8.5%-18.2% under DT-RT-SRT relative to RT-RT-RT. In the 0-20 cm soil layer, compared with the NPK+RT-RT-RT, soil urease, sucrase, and alkaline phosphatase activities increased by 4.0%-53.3%, 22.7%-71.3%, and 4.2%-57.2% under NPKM+DT-RT-SRT, respectively. The crop yield was increased under the treatment with combination of manure and rotational tillage modes. Compared with NPK, nitrogen accumulation in wheat increased but its nitrogen uptake and utilization reduced under NPKM condition. Compared with NPK+RT-RT-RT, in wheat season, the nitrogen accumulation in above-ground biomass, grain, and harvest index increased by 87.2%, 91.4%, and 30.3% under NPKM+DT-SRT-SRT, respectively. However, nitrogen use efficiency (NUE) and partial factor productivity decreased by 10.6% and 33.1%, respectively, while the nitrogen uptake efficiency in the subsequent maize season increased by 22.1%. Rotational tillage treatments reduced the apparent loss of nitrogen, with the best effect exhibiting under DT-SRT-SRT treatment. Compared with NPK, NPKM significantly increased crop nitrogen uptake and soil nitrogen residue, but the apparent nitrogen loss increased. The correlation analysis indicated, in the 0-40 cm soil layer, during wheat and maize seasons, BD was negatively correlated with crop yield, TN, NO3--N, NO4+-N, and other indicators content, while the nitrogen content was positively correlated with urease, sucrase, alkaline phosphatase activity, harvest index, and other indicators. Principle component analysis showed the effect of fertilization on soil physical, chemical and biological properties was more markable than that of tillage mode, and this effect was larger during wheat season than that during maize season.【Conclusion】In summary, the combination of manure and rotational tillage improved soil physical structure, increased the content of various forms of soil nitrogen, enhanced the soil enzymes activity, promoted nitrogen accumulation in plants, and increased crop yield, significantly reduced the apparent loss of soil nitrogen, which was conducive to the nitrogen balance of the soil. Therein, NPKM+DT-RT-SRT demonstrated the most effective improvement in both soil and crop quality. NPKM+DT-SRT-SRT exhibited the best improvement effect on the nitrogen efficiency index and the highest nitrogen utilization efficiency. Therefore, they were recommended as a suitable agricultural management in fluvo-aquic soil in the northern Henan Province.

Key words: fluvo-aquic soil, rotational tillage mode, organic fertilizer, nitrogen form, nitrogen balance

Fig. 1

The monthly accumulated precipitation and monthly average temperature during the experiment"

Table 1

Sowing and fertilization rate of wheat and maize"

作物
Crop
品种
Variety
播种量
Seeding rate
施肥量Fertilizer rate (kg·hm-2)
N P2O5 K2O
小麦Wheat 郑麦136 Zhengmai136 302.25 kg·hm-2 150+69 120 120
玉米Maize 浚单29 Xundan29 67500 plant/hm2 210 75 90

Fig. 2

Soil total nitrogen content in different soil layers under different treatments RT-RT-RT: Rotary tillage-rotary tillage-rotary tillage; DT-RT-RT: Deep tillage-rotary tillage-rotary tillage; DT-RT-SRT: Deep tillage-rotary tillage-shallow rotary tillage; DT-SRT-SRT: Deep tillage-shallow rotary tillage-shallow rotary tillage. NPK: Mineral fertilizer only; NPKM: NPK combined manure. Lowercase letters indicate significant differences between treatments (LSD, P<0.05). The same as below"

Fig. 3

Soil nitrate nitrogen content in different soil layers under different treatments"

Fig. 4

Soil ammonium nitrogen content in different soil layers under different treatments"

Fig. 5

The proportion of different forms of nitrogen in total nitrogen in different soil layers under different treatments"

Fig. 6

Soil bulk density and porosity of different soil layers under different treatments"

Fig. 7

Nitrogen accumulation in different parts of plants under different treatments"

Fig. 8

Soil urease, sucrase and alkaline phosphatase activity in different soil layers under different treatments"

Fig. 9

Yields of wheat and maize under different treatments"

Table 2

Nitrogen efficiency of wheat under different tillage and fertilization modes"

施肥处理Fertilizer treatment 耕作处理
Tillage treatment
地上部氮素积累量
Aboveground nitrogen accumulation (kg·hm-2)
收获指数
Harvest index
(kg·kg-1)
氮收获指数
N harvest index
(kg·kg-1)
氮肥吸收效率
N uptake efficiency
(kg·kg-1)
氮肥偏生产力
N partial factor productivity (kg·kg-1)
NPK RT-RT-RT 97.16±0.49d 0.66±0.01b 0.77±1.07b 0.44±0.02d 28.48±0.56c
DT-RT-RT 110.89±1.81c 0.72±0.01a 0.80±0.02a 0.51±0.08c 29.57±0.05c
DT-RT-SRT 142.29±1.3a 0.73±0.01a 0.80±0.05a 0.65±0.05a 32.90±0.46a
DT-SRT-SRT 134.09±1.33b 0.64±0.02b 0.78±0.02ab 0.61±0.04b 31.15±0.75b
NPKM RT-RT-RT 140.49±1.87d 0.74±0.01c 0.75±0.02c 0.33±0.02d 17.46±0.23c
DT-RT-RT 158.61±2.99c 0.78±0.03b 0.77±0.03b 0.37±0.03c 18.27±0.21b
DT-RT-SRT 168.71±3.92b 0.74±0.01c 0.77±0.01b 0.39±0.02b 18.44±0.22b
DT-SRT-SRT 181.91±0.82a 0.86±0.02a 0.79±0.08a 0.42±0.01a 19.06±0.23a

Table 3

Nitrogen efficiency of maize under different tillage and fertilization modes"

施肥处理
Fertilizer treatment
耕作处理
Tillage treatment
地上部氮素积累量
Aboveground nitrogen accumulation (kg·hm-2)
收获指数
Harvest index
(kg·kg-1)
氮收获指数
N harvest index
(kg·kg-1)
氮肥吸收效率
N uptake efficiency
(kg·kg-1)
氮肥偏生产力
N partial factor productivity (kg·kg-1)
NPK RT-RT-RT 236.25±0.76b 0.54±0.02c 0.52±0.01d 1.13±0.01b 46.65±0.34c
DT-RT-RT 237.86±2.84b 0.59±0.05b 0.62±0.02b 1.13±0.01b 49.90±0.48a
DT-RT-SRT 223.57±7.73c 0.62±0.02a 0.63±0.01a 1.06±0.04c 47.84±0.14b
DT-SRT-SRT 250.72±2.43a 0.61±0.03ab 0.54±0.01c 1.19±0.01a 49.54±0.20a
NPKM RT-RT-RT 273.95±2.65b 0.57±0.03c 0.60±0.01d 1.30±0.01b 48.50±0.28c
DT-RT-RT 256.68±5.29c 0.61±0.06b 0.68±0.01b 1.22±0.03c 48.90±0.76c
DT-RT-SRT 288.83±2.13a 0.66±0.06a 0.71±0.01a 1.38±0.01a 56.00±0.55a
DT-SRT-SRT 271.47±5.47b 0.65±0.03a 0.65±0.01c 1.29±0.03b 52.53±1.06b

Table 4

Nitrogen balance under different tillage and fertilization modes during the year of wheat and maize rotation (kg·hm-2)"

施肥处理Fertilizer treatment 耕作处理
Tillage
treatment
氮输入Nitrogen input 氮输出Nitrogen output 氮素表观
损失
Apparent
N loss
无机肥料
Mineral fertilizer
有机肥
Organic fertilizer
土壤矿物氮素
Soil mineralized N
秸秆还田氮素
Straw returning to field N
作物吸氮量
Crop nitrogen uptake
土壤残留氮素
N residue
in soil
NPK RT-RT-RT 429.0 0 148.1d 58.9b 311.3d 214.5c 110.2a
DT-RT-RT 429.0 0 204.3a 64.2ab 326.7c 265.6b 105.3a
DT-RT-SRT 429.0 0 189.0c 69.2a 337.7b 260.9b 88.7a
DT-SRT-SRT 429.0 0 194.9b 66.7ab 355.5a 307.3a 27.7b
NPKM RT-RT-RT 429.0 210.0 225.0c 72.3ab 380.4b 351.2b 204.7b
DT-RT-RT 429.0 210.0 256.3b 68.3b 379.1b 429.6a 155.0c
DT-RT-SRT 429.0 210.0 271.4a 77.4a 419.3a 337.4b 231.2a
DT-SRT-SRT 429.0 210.0 259.5b 78.4a 415.1a 409.5a 152.3c

Table 5

Multiple factors analysis of different indicators"

变量因素
Source of variation
施肥
Fertilizer
耕作
Tillage
季度
Season
施肥×耕作Fertilizer×
Tillage
施肥×季度Fertilizer×
Season
耕作×季度Tillage×
Season
施肥×耕作×季度
Fertilizer×Tillage×Season
全氮Total nitrogen 996.899** 213.273** 804.287** 22.070** 56.657** 30.000** 8.090**
硝态氮Nitrate nitrogen 554.638** 114.547** 305.312** 24.283** 72.991** 15.588** 17.376**
铵态氮Ammonium nitrogen 2.944NS 32.249** 167.478** 15.473** 340.475** 5.036** 13.947**
容重Bulk density 399.632** 39.061** 211.290** 5.363** 91.377** 11.322** 6.216**
孔隙度Porosity 763.178** 97.416** 263.068** 5.731** 79.573** 26.304** 6.267**
土壤脲酶Soil urease 134.923** 32.519** 953.479** 8.557** 19.148** 40.685** 16.489**
土壤蔗糖酶Soil sucrase 77.450** 21.563** 303.707** 1.293NS 0.001NS 9.503** 5.189**
土壤碱性磷酸酶
Soil alkaline phosphatase
28.039** 46.463** 3.274NS 0.702NS 0.189NS 31.595** 0.360NS
产量Yield 822.377** 133.400** 10588.189** 18.248** 74.096** 2.029NS 76.281**
地上部氮素积累量
Aboveground nitrogen accumulation
1587.314** 130.491** 13726.403** 9.207** 8.672** 70.704** 66.537**
氮肥偏生产力
N partial factor productivity
1092.669** 125.363** 33599.413** 19.303** 2974.798** 6.556** 90.500**
氮收获指数N harvest index 234.127** 195.289** 5651.412** 20.895** 521.760** 100.681** 1.484NS
氮肥吸收效率N uptake efficiency 0.297NS 94.086** 30201.171** 19.203** 1597.117** 43.345** 77.890**
收获指数Harvest index 15.932** 9.670** 402.772** 8.120** 38.226** 0.744NS 6.850**
氮素平衡Nitrogen balance 514.591** 51.471** 0.000NS 20.013** 0.000NS 0.000NS 0.000NS

Fig. 10

Correlation analysis of indicators in different soil layers under different treatments * Indicates correlation is significant (P<0.05), ** Indicates correlation is extremely significant (P<0.01). TN: Total nitrogen, NO3--N: Nitrate nitrogen, NH4+-N: Ammonium nitrogen, BD: Bulk density, POR: Porosity, S-UE: Soil urease activity, S-SC: Soil sucrase activity, S-ALP: Soil alkaline phosphatase activity, ANA: Aboveground nitrogen accumulation, NPFP: N partial factor productivity, NHI: N harvest index, NUE: N uptake efficiency, HI: Harvest index, NB: Nitrogen balance"

Fig. 11

Principal component analysis of soil physical, chemical and biological indexes under different treatments"

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